Detailed Information on Publication Record
2014
Repair of Site-Specific DNA Double-Strand Breaks in Barley Occurs via Diverse Pathways Primarily Involving the Sister Chromatid
VU, Giang T.H., Hieu X. CAO, Koichi WATANABE, Goetze HENSEL, Frank R. BLATTNER et. al.Basic information
Original name
Repair of Site-Specific DNA Double-Strand Breaks in Barley Occurs via Diverse Pathways Primarily Involving the Sister Chromatid
Authors
VU, Giang T.H. (276 Germany), Hieu X. CAO (276 Germany), Koichi WATANABE (276 Germany), Goetze HENSEL (276 Germany), Frank R. BLATTNER (276 Germany), Jochen KUMLEHN (276 Germany) and Ingo SCHUBERT (276 Germany, guarantor, belonging to the institution)
Edition
The Plant Cell, Rockville (USA), American Society of Plant Physiologists, 2014, 1040-4651
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 9.338
RIV identification code
RIV/00216224:14740/14:00077479
Organization unit
Central European Institute of Technology
UT WoS
000338771700030
Keywords in English
SOMATIC PLANT-CELLS; HOMOLOGOUS RECOMBINATION; PHYSCOMITRELLA-PATENS; GENOME EVOLUTION; SEQUENCES; EXCHANGES; MECHANISM; REPLICATION; CONVERSION; CHOICE
Tags
Tags
International impact, Reviewed
Změněno: 26/11/2014 07:46, Martina Prášilová
Abstract
V originále
DNA double-strand break (DSB) repair mechanisms differ in their requirements for a homologous repair template and in the accuracy of the result. We aimed to quantify the outcome of repair of a single targeted DSB in somatic cells of young barley (Hordeum vulgare) plants. Amplicon sequencing of three reporter constructs revealed 47 to 58% of reads as repaired via nonhomologous end-joining (NHEJ) with deletions and/or small (1 to 3 bp) insertions. Alternative NHEJ revealed 2 to 5 bp microhomology (15.7% of cases) or new replication-mediated short duplications at sealed breaks. Although deletions outweigh insertions in barley, this bias was less pronounced and deleted sequences were shorter than in Arabidopsis thaliana. Between 17 and 33% of reads likely represent restoration of the original sequence. Depending on the construct, 20 to 33% of reads arose via gene conversion (homologous recombination). Remarkably, < 1 to > 8% of reads apparently display synthesis-dependent strand annealing linked with NHEJ, inserting 4 to 61 bp, mostly originating from the surrounding of breakpoints. Positional coincidence of > 81% of sister chromatid exchanges with target loci is unprecedented for higher eukaryotes and indicates that most repair events for staggered DSBs, at least in barley, involve the sister chromatid and occur during S or G2 phase of the cell cycle.
Links
EE2.3.20.0189, research and development project |
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