Loading of the centromeric histone H3 variant during
meiosis-how does it differ from mitosis?

J 2014

Loading of the centromeric histone H3 variant during meiosis-how does it differ from mitosis?

SCHUBERT, Veit, Inna LERMONTOVA and Ingo SCHUBERT

Basic information

Original name

Loading of the centromeric histone H3 variant during meiosis-how does it differ from mitosis?

Authors

SCHUBERT, Veit (276 Germany), Inna LERMONTOVA (276 Germany) and Ingo SCHUBERT (276 Germany, guarantor, belonging to the institution)

Edition

Chromosoma, New York, Springer, 2014, 0009-5915

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Full Text

Impact factor

Impact factor: 4.602

RIV identification code

RIV/00216224:14740/14:00077480

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1007/s00412-014-0466-9

UT WoS

000342465800008

Keywords in English

CELL-CYCLE PROGRESSION; CENP-A; CHROMOSOME; DYNAMICS; HETEROCHROMATIN; SEGREGATION; REQUIRES; PLANTS

Abstract

V originále

In eukaryotic phyla studied so far, the essential centromeric histone H3 variant (CENH3) is loaded to centromeric nucleosomes after S-phase (except for yeast) but before mitotic segregation (except for metazoan). While the C-terminal part of CENH3 seems to be sufficient for mitotic centromere function in plants, meiotic centromeres neither load nor tolerate impaired CENH3 molecules. However, details about CENH3 deposition in meiocytes are unknown (except for Drosophila). Therefore, we quantified fluorescence signals after the immunostaining of CENH3 along meiotic and mitotic nuclear division cycles of rye, a monocotyledonous plant. One peak of fluorescence intensity appeared in the early meiotic prophase of pollen mother cells and a second one during interkinesis, both followed by a decrease of CENH3. Then, the next loading occurred in the male gametophyte before its first mitotic division. These data indicate that CENH3 loading differs between mitotic and meiotic nuclei. Contrary to the situation in mitotic cycles, CENH3 deposition is biphasic during meiosis and apparently linked with a quality check, a removal of impaired CENH3 molecules, and a general loss of CENH3 after each loading phase. These steps ensure an endowment of centromeres with a sufficient amount of correct CENH3 molecules as a prerequisite for centromere maintenance during mitotic cycles of the microgametophyte and the progeny. From a comparison with data available for Drosophila, we hypothesise that the post-divisional mitotic CENH3 loading in metazoans is evolutionarily derived from the post-divisional meiotic loading phase, while the pre-divisional first meiotic loading has been conserved among eukaryotes.

Links

EE2.3.20.0189, research and development project

Name: Rozvoj výzkumné excelence v oblasti evoluční cytogenomiky, epigenetiky a buněčné signalizace

Citovat

SCHUBERT, Veit, Inna LERMONTOVA and Ingo SCHUBERT. Loading of the centromeric histone H3 variant during meiosis-how does it differ from mitosis? Chromosoma. New York: Springer, 2014, vol. 123, No 5, p. 491-497. ISSN 0009-5915. Available from: https://dx.doi.org/10.1007/s00412-014-0466-9.

@article{1210518,
   author = {Schubert, Veit and Lermontova, Inna and Schubert, Ingo},
   article_location = {New York},
   article_number = {5},
   doi = {http://dx.doi.org/10.1007/s00412-014-0466-9},
   keywords = {CELL-CYCLE PROGRESSION; CENP-A; CHROMOSOME; DYNAMICS; HETEROCHROMATIN; SEGREGATION; REQUIRES; PLANTS},
   language = {eng},
   issn = {0009-5915},
   journal = {Chromosoma},
   title = {Loading of the centromeric histone H3 variant during meiosis-how does it differ from mitosis?},
   url = {http://www.ncbi.nlm.nih.gov/pubmed/24806806},
   volume = {123},
   year = {2014}
}

TY  - JOUR
ID  - 1210518
AU  - Schubert, Veit - Lermontova, Inna - Schubert, Ingo
PY  - 2014
TI  - Loading of the centromeric histone H3 variant during meiosis-how does it differ from mitosis?
JF  - Chromosoma
VL  - 123
IS  - 5
SP  - 491-497
EP  - 491-497
PB  - Springer
SN  - 00095915
KW  - CELL-CYCLE PROGRESSION
KW  - CENP-A
KW  - CHROMOSOME
KW  - DYNAMICS
KW  - HETEROCHROMATIN
KW  - SEGREGATION
KW  - REQUIRES
KW  - PLANTS
UR  - http://www.ncbi.nlm.nih.gov/pubmed/24806806
L2  - http://www.ncbi.nlm.nih.gov/pubmed/24806806
N2  - In eukaryotic phyla studied so far, the essential centromeric histone H3 variant (CENH3) is loaded to centromeric nucleosomes after S-phase (except for yeast) but before mitotic segregation (except for metazoan). While the C-terminal part of CENH3 seems to be sufficient for mitotic centromere function in plants, meiotic centromeres neither load nor tolerate impaired CENH3 molecules. However, details about CENH3 deposition in meiocytes are unknown (except for Drosophila). Therefore, we quantified fluorescence signals after the immunostaining of CENH3 along meiotic and mitotic nuclear division cycles of rye, a monocotyledonous plant. One peak of fluorescence intensity appeared in the early meiotic prophase of pollen mother cells and a second one during interkinesis, both followed by a decrease of CENH3. Then, the next loading occurred in the male gametophyte before its first mitotic division. These data indicate that CENH3 loading differs between mitotic and meiotic nuclei. Contrary to the situation in mitotic cycles, CENH3 deposition is biphasic during meiosis and apparently linked with a quality check, a removal of impaired CENH3 molecules, and a general loss of CENH3 after each loading phase. These steps ensure an endowment of centromeres with a sufficient amount of correct CENH3 molecules as a prerequisite for centromere maintenance during mitotic cycles of the microgametophyte and the progeny. From a comparison with data available for Drosophila, we hypothesise that the post-divisional mitotic CENH3 loading in metazoans is evolutionarily derived from the post-divisional meiotic loading phase, while the pre-divisional first meiotic loading has been conserved among eukaryotes.
ER  -

SCHUBERT, Veit, Inna LERMONTOVA and Ingo SCHUBERT. Loading of the centromeric histone H3 variant during meiosis-how does it differ from mitosis? \textit{Chromosoma}. New York: Springer, 2014, vol.~123, No~5, p.~491-497. ISSN~0009-5915. Available from: https://dx.doi.org/10.1007/s00412-014-0466-9.

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