When fathers are instant losers: homogenization of rDNA loci in
recently formed Cardamine X schulzii trigenomic allopolyploid

J 2014

When fathers are instant losers: homogenization of rDNA loci in recently formed Cardamine X schulzii trigenomic allopolyploid

ZOZOMOVÁ-LIHOVÁ, Judita, Terezie MANDÁKOVÁ, Alena KOVAŘÍKOVÁ, Andreas MÜHLHAUSEN, Klaus MUMMENHOFF et. al.

Basic information

Original name

When fathers are instant losers: homogenization of rDNA loci in recently formed Cardamine X schulzii trigenomic allopolyploid

Authors

ZOZOMOVÁ-LIHOVÁ, Judita (703 Slovakia), Terezie MANDÁKOVÁ (203 Czech Republic, belonging to the institution), Alena KOVAŘÍKOVÁ (203 Czech Republic), Andreas MÜHLHAUSEN (276 Germany), Klaus MUMMENHOFF (276 Germany), Martin LYSÁK (203 Czech Republic, guarantor, belonging to the institution) and Aleš KOVAŘÍK (203 Czech Republic)

Edition

New Phytologist, Hoboken, WILEY-BLACKWELL, 2014, 0028-646X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 7.672

RIV identification code

RIV/00216224:14740/14:00074135

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1111/nph.12873

UT WoS

000340286000010

Keywords in English

NUCLEAR RIBOSOMAL DNA; RNA GENE SITES; CONCERTED EVOLUTION; TRAGOPOGON ASTERACEAE; NATURAL-POPULATIONS; SENECIO-CAMBRENSIS; MOLECULAR EVIDENCE; BRASSICA-NAPUS; PARENTAL RDNA; GENOME

Abstract

V originále

Recently formed allopolyploids represent an excellent system to study the impacts of hybridization and genomic duplication on genome structure and evolution. Here we explored the 35S rRNA genes (rDNA) in the Cardamine x schulzii allohexaploid that was formed by two subsequent hybridization events within the past c. 150 yr. The rDNA loci were analyzed by cloning, next generation sequencing (NGS), RT-PCR and FISH methods. The primary C. x insueta triploid hybrid derived from C. rivularis (female) and C. amara (male) had gene ratios highly skewed towards maternal sequences. Similarly, C. x schulzii, originating from the secondary hybridization event involving C. x insueta (female) and C. pratensis (male), showed a reduction in paternal rDNA homeologs despite an excess of chromosomes inherited from C. pratensis. We also identified novel rDNA loci in C. x schulzii, suggesting that lost loci might be slowly reinstalled by translocation (but not recombination) of genes from partner genomes. Prevalent clonal propagation of allopolyploids, C. x insueta and C. x schulzii, indicates that concerted evolution of rDNA may occur in the absence of extensive meiotic cycles. Adoption of NGS in rDNA variant analysis is highly informative for deciphering the evolutionary histories of allopolyploid species with ongoing homogenization processes.

Links

EE2.3.20.0189, research and development project

Name: Rozvoj výzkumné excelence v oblasti evoluční cytogenomiky, epigenetiky a buněčné signalizace

EE2.3.30.0037, research and development project

Name: Zaměstnáním nejlepších mladých vědců k rozvoji mezinárodní spolupráce

GBP501/12/G090, research and development project

Name: Evoluce a funkce komplexních genomů rostlin

Citovat

ZOZOMOVÁ-LIHOVÁ, Judita, Terezie MANDÁKOVÁ, Alena KOVAŘÍKOVÁ, Andreas MÜHLHAUSEN, Klaus MUMMENHOFF, Martin LYSÁK and Aleš KOVAŘÍK. When fathers are instant losers: homogenization of rDNA loci in recently formed Cardamine X schulzii trigenomic allopolyploid. New Phytologist. Hoboken: WILEY-BLACKWELL, 2014, vol. 203, No 4, p. 1096-1108. ISSN 0028-646X. Available from: https://dx.doi.org/10.1111/nph.12873.

@article{1210545,
   author = {ZozomováandLihová, Judita and Mandáková, Terezie and Kovaříková, Alena and Mühlhausen, Andreas and Mummenhoff, Klaus and Lysák, Martin and Kovařík, Aleš},
   article_location = {Hoboken},
   article_number = {4},
   doi = {http://dx.doi.org/10.1111/nph.12873},
   keywords = {NUCLEAR RIBOSOMAL DNA; RNA GENE SITES; CONCERTED EVOLUTION; TRAGOPOGON ASTERACEAE; NATURAL-POPULATIONS; SENECIO-CAMBRENSIS; MOLECULAR EVIDENCE; BRASSICA-NAPUS; PARENTAL RDNA; GENOME},
   language = {eng},
   issn = {0028-646X},
   journal = {New Phytologist},
   title = {When fathers are instant losers: homogenization of rDNA loci in recently formed Cardamine X schulzii trigenomic allopolyploid},
   url = {http://onlinelibrary.wiley.com/doi/10.1111/nph.12873/epdf},
   volume = {203},
   year = {2014}
}

TY  - JOUR
ID  - 1210545
AU  - Zozomová-Lihová, Judita - Mandáková, Terezie - Kovaříková, Alena - Mühlhausen, Andreas - Mummenhoff, Klaus - Lysák, Martin - Kovařík, Aleš
PY  - 2014
TI  - When fathers are instant losers: homogenization of rDNA loci in recently formed Cardamine X schulzii trigenomic allopolyploid
JF  - New Phytologist
VL  - 203
IS  - 4
SP  - 1096-1108
EP  - 1096-1108
PB  - WILEY-BLACKWELL
SN  - 0028646X
KW  - NUCLEAR RIBOSOMAL DNA
KW  - RNA GENE SITES
KW  - CONCERTED EVOLUTION
KW  - TRAGOPOGON ASTERACEAE
KW  - NATURAL-POPULATIONS
KW  - SENECIO-CAMBRENSIS
KW  - MOLECULAR EVIDENCE
KW  - BRASSICA-NAPUS
KW  - PARENTAL RDNA
KW  - GENOME
UR  - http://onlinelibrary.wiley.com/doi/10.1111/nph.12873/epdf
L2  - http://onlinelibrary.wiley.com/doi/10.1111/nph.12873/epdf
N2  - Recently formed allopolyploids represent an excellent system to study the impacts of hybridization and genomic duplication on genome structure and evolution. Here we explored the 35S rRNA genes (rDNA) in the Cardamine x schulzii allohexaploid that was formed by two subsequent hybridization events within the past c. 150 yr. The rDNA loci were analyzed by cloning, next generation sequencing (NGS), RT-PCR and FISH methods. The primary C. x insueta triploid hybrid derived from C. rivularis (female) and C. amara (male) had gene ratios highly skewed towards maternal sequences. Similarly, C. x schulzii, originating from the secondary hybridization event involving C. x insueta (female) and C. pratensis (male), showed a reduction in paternal rDNA homeologs despite an excess of chromosomes inherited from C. pratensis. We also identified novel rDNA loci in C. x schulzii, suggesting that lost loci might be slowly reinstalled by translocation (but not recombination) of genes from partner genomes. Prevalent clonal propagation of allopolyploids, C. x insueta and C. x schulzii, indicates that concerted evolution of rDNA may occur in the absence of extensive meiotic cycles. Adoption of NGS in rDNA variant analysis is highly informative for deciphering the evolutionary histories of allopolyploid species with ongoing homogenization processes.
ER  -

ZOZOMOVÁ-LIHOVÁ, Judita, Terezie MANDÁKOVÁ, Alena KOVAŘÍKOVÁ, Andreas MÜHLHAUSEN, Klaus MUMMENHOFF, Martin LYSÁK and Aleš KOVAŘÍK. When fathers are instant losers: homogenization of rDNA loci in recently formed Cardamine X schulzii trigenomic allopolyploid. \textit{New Phytologist}. Hoboken: WILEY-BLACKWELL, 2014, vol.~203, No~4, p.~1096-1108. ISSN~0028-646X. Available from: https://dx.doi.org/10.1111/nph.12873.

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