Detailed Information on Publication Record
2014
Guanine quadruplexes are formed by specific regions of human transposable elements
LEXA, Matej, Pavlina STEFLOVA, Tomáš MARTÍNEK, Michaela VORLÍČKOVÁ, Boris VYSKOT et. al.Basic information
Original name
Guanine quadruplexes are formed by specific regions of human transposable elements
Authors
LEXA, Matej (703 Slovakia, guarantor, belonging to the institution), Pavlina STEFLOVA (203 Czech Republic), Tomáš MARTÍNEK (203 Czech Republic), Michaela VORLÍČKOVÁ (203 Czech Republic, belonging to the institution), Boris VYSKOT (203 Czech Republic) and Eduard KEJNOVSKÝ (203 Czech Republic)
Edition
BMC Genomics, London, BioMed Central Ltd, 2014, 1471-2164
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10602 Biology , Evolutionary biology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 3.986
RIV identification code
RIV/00216224:14330/14:00077524
Organization unit
Faculty of Informatics
UT WoS
000355992300001
Keywords (in Czech)
G4 kvadruplex; lidsky genom; transposony; cirkularni dichroismus; Bioconductor; bioinformatika
Keywords in English
G4 quadruplex; human transposable elements; circular dichroism; Bioconductor code; bioinformatics
Tags
International impact, Reviewed
Změněno: 13/3/2018 14:08, doc. Ing. Matej Lexa, Ph.D.
Abstract
V originále
BACKGROUND:Transposable elements form a significant proportion of eukaryotic genomes. Recently, Lexa et al (Nucleic Acids Res 42:968-978, 2014) reported that plant long terminal repeat (LTR) retrotransposons often contain potential quadruplex sequences (PQSs) in their LTRs and experimentally confirmed their ability to adopt four-stranded DNA conformations.RESULTS:Here, we searched for PQSs in human retrotransposons and found that PQSs are specifically localized in the 3'-UTR of LINE-1 elements, in LTRs of HERV elements and are strongly accumulated in specific regions of SVA elements. Circular dichroism spectroscopy confirmed that most PQSs had adopted monomolecular or bimolecular guanine quadruplex structures. Evolutionarily young SVA elements contained more PQSs than older elements and their propensity to form quadruplex DNA was higher. Full-length L1 elements contained more PQSs than truncated elements; the highest proportion of PQSs was found inside transpositionally active L1 elements (PA2 and HS families).CONCLUSIONS:Conservation of quadruplexes at specific positions of transposable elements implies their importance in their life cycle. The increasing quadruplex presence in evolutionary young LINE-1 and SVA families makes these elements important contributors toward present genome-wide quadruplex distribution.
Links
| CZ.1.05/1.1.00/02.0068, interní kód MU |
|