Do variability and expression level of fish MHC genes play
crucial role in parasite load and host immunocompetence?

D 2014

Do variability and expression level of fish MHC genes play crucial role in parasite load and host immunocompetence?

CIVÁŇOVÁ, Kristína

Basic information

Original name

Do variability and expression level of fish MHC genes play crucial role in parasite load and host immunocompetence?

Authors

CIVÁŇOVÁ, Kristína (703 Slovakia, guarantor, belonging to the institution)

Edition

Brno, 3rd Workshop of European Centre of Ichthyoparasitology, p. 10-11, 2 pp. 2014

Publisher

MUNI Press

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

printed version "print"

RIV identification code

RIV/00216224:14310/14:00074146

Organization unit

Faculty of Science

ISBN

978-80-210-7492-7

Keywords in English

MHC; gene expression; HRM; Cyprinidae

Změněno: 20/2/2018 13:22, RNDr. Kristína Křížová, Ph.D.

Abstract

V originále

We have confirmed extremely high level of allelic diversity in several fish genera by classical genotyping. We used two basic approaches for this purpose. First, the older, was CE-SSCP (Capillary Electrophoresis-Single Stranded Conformational Polymorphism) of PCR amplicon clones combined with automatic sequencing based on Sanger technology (ABI 3130 Genetic Analyzer). The second, modern, was 454 clonal pyrosequencing of heterozygous amplicons (454 GS FLX platform with Titanium chemistry). Recently, using CFX96 real time PCR system, we test also the expression levels of fish MHC genes (DAB1 and DAB3) by quantitative real-time PCR (qPCR) and we launched a new approach for MHC genes variability tests - high resolution melting analysis (HRM), too. After completing "HRM allele profile database" and finish analyses of qPCR we will be exploring associations with parasite load in chosen Cyprinid species.

Links

GBP505/12/G112, research and development project

Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation

Citovat

CIVÁŇOVÁ, Kristína. Do variability and expression level of fish MHC genes play crucial role in parasite load and host immunocompetence? In Šárka Mašová, Milan Gelnar. 3rd Workshop of European Centre of Ichthyoparasitology. Brno: MUNI Press, 2014, p. 10-11. ISBN 978-80-210-7492-7.

@inproceedings{1210760,
   author = {Civáňová, Kristína},
   address = {Brno},
   booktitle = {3rd Workshop of European Centre of Ichthyoparasitology},
   editor = {Šárka Mašová, Milan Gelnar},
   keywords = {MHC; gene expression; HRM; Cyprinidae},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Brno},
   isbn = {978-80-210-7492-7},
   pages = {10-11},
   publisher = {MUNI Press},
   title = {Do variability and expression level of fish MHC genes play crucial role in parasite load and host immunocompetence?},
   year = {2014}
}

TY  - JOUR
ID  - 1210760
AU  - Civáňová, Kristína
PY  - 2014
TI  - Do variability and expression level of fish MHC genes play crucial role in parasite load and host immunocompetence?
PB  - MUNI Press
CY  - Brno
SN  - 9788021074927
KW  - MHC
KW  - gene expression
KW  - HRM
KW  - Cyprinidae
N2  - We have confirmed extremely high level of allelic diversity in several fish genera by classical genotyping. We used two basic approaches for this purpose. First, the older, was CE-SSCP (Capillary Electrophoresis-Single Stranded Conformational Polymorphism) of PCR amplicon clones combined with automatic sequencing based on Sanger technology (ABI 3130 Genetic Analyzer). The second, modern, was 454 clonal pyrosequencing of heterozygous amplicons (454 GS FLX platform with Titanium chemistry). Recently, using CFX96 real time PCR system, we test also the expression levels of fish MHC genes (DAB1 and DAB3) by quantitative real-time PCR (qPCR) and we launched a new approach for MHC genes variability tests - high resolution melting analysis (HRM), too. After completing "HRM allele profile database" and finish analyses of qPCR we will be exploring associations with parasite load in chosen Cyprinid species.
ER  -

CIVÁŇOVÁ, Kristína. Do variability and expression level of fish MHC genes play crucial role in parasite load and host immunocompetence? In Šárka Mašová, Milan Gelnar. \textit{3rd Workshop of European Centre of Ichthyoparasitology}. Brno: MUNI Press, 2014, p.~10-11. ISBN~978-80-210-7492-7.

Detailed Information on Publication Record