Do variability and expression level of fish MHC genes play crucial role in parasite load and host immunocompetence?

CIVÁŇOVÁ, Kristína. Do variability and expression level of fish MHC genes play crucial role in parasite load and host immunocompetence? In Šárka Mašová, Milan Gelnar. 3rd Workshop of European Centre of Ichthyoparasitology. Brno: MUNI Press, 2014, p. 10-11. ISBN 978-80-210-7492-7.

Basic information

• Original name: Do variability and expression level of fish MHC genes play crucial role in parasite load and host immunocompetence?
• Authors: CIVÁŇOVÁ, Kristína (703 Slovakia, guarantor, belonging to the institution).
• Edition: Brno, 3rd Workshop of European Centre of Ichthyoparasitology, p. 10-11, 2 pp. 2014.
• Publisher: MUNI Press

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• Publication form: printed version "print"
• RIV identification code: RIV/00216224:14310/14:00074146
• Organization unit: Faculty of Science
• ISBN: 978-80-210-7492-7
• Keywords in English: MHC; gene expression; HRM; Cyprinidae

Abstract

We have confirmed extremely high level of allelic diversity in several fish genera by classical genotyping. We used two basic approaches for this purpose. First, the older, was CE-SSCP (Capillary Electrophoresis-Single Stranded Conformational Polymorphism) of PCR amplicon clones combined with automatic sequencing based on Sanger technology (ABI 3130 Genetic Analyzer). The second, modern, was 454 clonal pyrosequencing of heterozygous amplicons (454 GS FLX platform with Titanium chemistry). Recently, using CFX96 real time PCR system, we test also the expression levels of fish MHC genes (DAB1 and DAB3) by quantitative real-time PCR (qPCR) and we launched a new approach for MHC genes variability tests - high resolution melting analysis (HRM), too. After completing "HRM allele profile database" and finish analyses of qPCR we will be exploring associations with parasite load in chosen Cyprinid species.

Links

• GBP505/12/G112, research and development project: Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation