Whole Genome Sequence of the Treponema pallidum subsp.
endemicum Strain Bosnia A: The Genome Is Related to Yaws
Treponemes but Contains Few Loci Similar to Syphilis Treponemes

J 2014

Whole Genome Sequence of the Treponema pallidum subsp. endemicum Strain Bosnia A: The Genome Is Related to Yaws Treponemes but Contains Few Loci Similar to Syphilis Treponemes

ŠTAUDOVÁ, Barbora, Michal STROUHAL, Marie ZOBANÍKOVÁ, Darina ČEJKOVÁ, Lucinda L. FULTON et. al.

Basic information

Original name

Whole Genome Sequence of the Treponema pallidum subsp. endemicum Strain Bosnia A: The Genome Is Related to Yaws Treponemes but Contains Few Loci Similar to Syphilis Treponemes

Authors

ŠTAUDOVÁ, Barbora (203 Czech Republic, belonging to the institution), Michal STROUHAL (203 Czech Republic, belonging to the institution), Marie ZOBANÍKOVÁ (203 Czech Republic, belonging to the institution), Darina ČEJKOVÁ (203 Czech Republic, belonging to the institution), Lucinda L. FULTON (840 United States of America), Lei CHEN (840 United States of America), lorenzo GIACANI (840 United States of America), Arturo CENTURION-LARA (840 United States of America), Sylvia M. BRUISTEN (528 Netherlands), Erica SODERGREN (840 United States of America), George M. WEINSTOCK (840 United States of America) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)

Edition

PLoS neglected tropical diseases, San Francisco, Public Library of Science, 2014, 1935-2735

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 4.446

RIV identification code

RIV/00216224:14110/14:00074160

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1371/journal.pntd.0003261

UT WoS

000345514000016

Keywords in English

Treponema pallidum; endemic syphilis

Abstract

V originále

Background: T. pallidum subsp. endemicum (TEN) is the causative agent of bejel (also known as endemic syphilis). Clinical symptoms of syphilis and bejel are overlapping and the epidemiological context is important for correct diagnosis of both diseases. In contrast to syphilis, caused by T. pallidum subsp. pallidum (TPA), TEN infections are usually spread by direct contact or contaminated utensils rather than by sexual contact. Bejel is most often seen in western Africa and in the Middle East. The strain Bosnia A was isolated in 1950 in Bosnia, southern Europe. Methodology/Principal Findings: The complete genome of the Bosnia A strain was amplified and sequenced using the pooled segment genome sequencing (PSGS) method and a combination of three next-generation sequencing techniques (SOLiD, Roche 454, and Illumina). Using this approach, a total combined average genome coverage of 5136was achieved. The size of the Bosnia A genome was found to be 1,137,653 bp, i.e. 1.6–2.8 kbp shorter than any previously published genomes of uncultivable pathogenic treponemes. Conserved gene synteny was found in the Bosnia A genome compared to other sequenced syphilis and yaws treponemes. The TEN Bosnia A genome was distinct but very similar to the genome of yaws-causing T. pallidum subsp. pertenue (TPE) strains. Interestingly, the TEN Bosnia A genome was found to contain several sequences, which so far, have been uniquely identified only in syphilis treponemes. Conclusions/Significance: The genome of TEN Bosnia A contains several sequences thought to be unique to TPA strains; these sequences very likely represent remnants of recombination events during the evolution of TEN treponemes. This finding emphasizes a possible role of repeated horizontal gene transfer between treponemal subspecies in shaping the Bosnia A genome.

Links

EE2.3.30.0009, research and development project

Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GAP302/12/0574, research and development project

Name: Celogenomové sekvencování v analýze genomů a transkriptomů patogenních bakterií rodu Treponema

Investor: Czech Science Foundation

NT11159, research and development project

Name: Mapování výskytu makrolidové rezistence původce syfilis v ČR a molekulární typizace jednotlivých syfilitických kmenů

Investor: Ministry of Health of the CR

Citovat

ŠTAUDOVÁ, Barbora, Michal STROUHAL, Marie ZOBANÍKOVÁ, Darina ČEJKOVÁ, Lucinda L. FULTON, Lei CHEN, lorenzo GIACANI, Arturo CENTURION-LARA, Sylvia M. BRUISTEN, Erica SODERGREN, George M. WEINSTOCK and David ŠMAJS. Whole Genome Sequence of the Treponema pallidum subsp. endemicum Strain Bosnia A: The Genome Is Related to Yaws Treponemes but Contains Few Loci Similar to Syphilis Treponemes. PLoS neglected tropical diseases. San Francisco: Public Library of Science, 2014, vol. 8, No 11, p. "e3261", 13 pp. ISSN 1935-2735. Available from: https://dx.doi.org/10.1371/journal.pntd.0003261.

@article{1211569,
   author = {Štaudová, Barbora and Strouhal, Michal and Zobaníková, Marie and Čejková, Darina and Fulton, Lucinda L. and Chen, Lei and Giacani, lorenzo and CenturionandLara, Arturo and Bruisten, Sylvia M. and Sodergren, Erica and Weinstock, George M. and Šmajs, David},
   article_location = {San Francisco},
   article_number = {11},
   doi = {http://dx.doi.org/10.1371/journal.pntd.0003261},
   keywords = {Treponema pallidum; endemic syphilis},
   language = {eng},
   issn = {1935-2735},
   journal = {PLoS neglected tropical diseases},
   title = {Whole Genome Sequence of the Treponema pallidum subsp. endemicum Strain Bosnia A: The Genome Is Related to Yaws Treponemes but Contains Few Loci Similar to Syphilis Treponemes},
   volume = {8},
   year = {2014}
}

TY  - JOUR
ID  - 1211569
AU  - Štaudová, Barbora - Strouhal, Michal - Zobaníková, Marie - Čejková, Darina - Fulton, Lucinda L. - Chen, Lei - Giacani, lorenzo - Centurion-Lara, Arturo - Bruisten, Sylvia M. - Sodergren, Erica - Weinstock, George M. - Šmajs, David
PY  - 2014
TI  - Whole Genome Sequence of the Treponema pallidum subsp. endemicum Strain Bosnia A: The Genome Is Related to Yaws Treponemes but Contains Few Loci Similar to Syphilis Treponemes
JF  - PLoS neglected tropical diseases
VL  - 8
IS  - 11
SP  - "e3261"
EP  - "e3261"
PB  - Public Library of Science
SN  - 19352735
KW  - Treponema pallidum
KW  - endemic syphilis
N2  - Background: T. pallidum subsp. endemicum (TEN) is the causative agent of bejel (also known as endemic syphilis). Clinical symptoms of syphilis and bejel are overlapping and the epidemiological context is important for correct diagnosis of both diseases. In contrast to syphilis, caused by T. pallidum subsp. pallidum (TPA), TEN infections are usually spread by direct contact or contaminated utensils rather than by sexual contact. Bejel is most often seen in western Africa and in the Middle East. The strain Bosnia A was isolated in 1950 in Bosnia, southern Europe. Methodology/Principal Findings: The complete genome of the Bosnia A strain was amplified and sequenced using the pooled segment genome sequencing (PSGS) method and a combination of three next-generation sequencing techniques (SOLiD, Roche 454, and Illumina). Using this approach, a total combined average genome coverage of 5136was achieved. The size of the Bosnia A genome was found to be 1,137,653 bp, i.e. 1.6–2.8 kbp shorter than any previously published genomes of uncultivable pathogenic treponemes. Conserved gene synteny was found in the Bosnia A genome compared to other sequenced syphilis and yaws treponemes. The TEN Bosnia A genome was distinct but very similar to the genome of yaws-causing T. pallidum subsp. pertenue (TPE) strains. Interestingly, the TEN Bosnia A genome was found to contain several sequences, which so far, have been uniquely identified only in syphilis treponemes. Conclusions/Significance: The genome of TEN Bosnia A contains several sequences thought to be unique to TPA strains; these sequences very likely represent remnants of recombination events during the evolution of TEN treponemes. This finding emphasizes a possible role of repeated horizontal gene transfer between treponemal subspecies in shaping the Bosnia A genome.
ER  -

ŠTAUDOVÁ, Barbora, Michal STROUHAL, Marie ZOBANÍKOVÁ, Darina ČEJKOVÁ, Lucinda L. FULTON, Lei CHEN, lorenzo GIACANI, Arturo CENTURION-LARA, Sylvia M. BRUISTEN, Erica SODERGREN, George M. WEINSTOCK and David ŠMAJS. Whole Genome Sequence of the Treponema pallidum subsp. endemicum Strain Bosnia A: The Genome Is Related to Yaws Treponemes but Contains Few Loci Similar to Syphilis Treponemes. \textit{PLoS neglected tropical diseases}. San Francisco: Public Library of Science, 2014, vol.~8, No~11, p.~''e3261'', 13 pp. ISSN~1935-2735. Available from: https://dx.doi.org/10.1371/journal.pntd.0003261.

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