| BALÁŽOVÁ, Tereza, Ondrej ŠEDO, Polonca ŠTEFANIC, Ines MANDIC-MULEC, Michiel VOS and Zbyněk ZDRÁHAL. Improvement in Staphylococcus and Bacillus strain differentiation by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling by using microwave-assisted enzymatic digestion. Rapid Communications in Mass Spectrometry. Hoboken (USA): Wiley-Blackwell, 2014, vol. 28, No 17, p. 1855-1861. ISSN 0951-4198. Available from: https://dx.doi.org/10.1002/rcm.6966. |
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@article{1211680,
author = {Balážová, Tereza and Šedo, Ondrej and Štefanic, Polonca and MandicandMulec, Ines and Vos, Michiel and Zdráhal, Zbyněk},
article_location = {Hoboken (USA)},
article_number = {17},
doi = {http://dx.doi.org/10.1002/rcm.6966},
keywords = {proteomics; bacteria; identification; bacillus subtilis},
language = {eng},
issn = {0951-4198},
journal = {Rapid Communications in Mass Spectrometry},
title = {Improvement in Staphylococcus and Bacillus strain differentiation by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling by using microwave-assisted enzymatic digestion},
url = {http://onlinelibrary.wiley.com.ezproxy.muni.cz/doi/10.1002/rcm.6966/abstract;jsessionid=3C620400539588CB37DB7BEF515359BD.f01t01},
volume = {28},
year = {2014}
}
TY - JOUR
ID - 1211680
AU - Balážová, Tereza - Šedo, Ondrej - Štefanic, Polonca - Mandic-Mulec, Ines - Vos, Michiel - Zdráhal, Zbyněk
PY - 2014
TI - Improvement in Staphylococcus and Bacillus strain differentiation by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling by using microwave-assisted enzymatic digestion
JF - Rapid Communications in Mass Spectrometry
VL - 28
IS - 17
SP - 1855-1861
EP - 1855-1861
PB - Wiley-Blackwell
SN - 09514198
KW - proteomics
KW - bacteria
KW - identification
KW - bacillus subtilis
UR - http://onlinelibrary.wiley.com.ezproxy.muni.cz/doi/10.1002/rcm.6966/abstract;jsessionid=3C620400539588CB37DB7BEF515359BD.f01t01
L2 - http://onlinelibrary.wiley.com.ezproxy.muni.cz/doi/10.1002/rcm.6966/abstract;jsessionid=3C620400539588CB37DB7BEF515359BD.f01t01
N2 - RATIONALE: Distinguishing between individual bacterial strains below the species level is a challenge to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) bacterial profiling. We propose a quick method for improving strain differentiation of two Staphylococcus and one Bacillus species. METHODS: An alternative procedure to the extraction protocol recommended by Bruker Daltonics was developed. Ethanol-sterilized cells of six S. aureus and six S. haemolyticus strains were digested by trypsin using 2-min microwave irradiation and were then analyzed. Twenty-eight strains belonging to two ecotypes of B. subtilis were subjected to the same procedure to extend the scope of the method. RESULTS: S. aureus and S. haemolyticus strains, only partially distinguishable by the standard sample preparation procedure, were subjected to microwave-assisted tryptic digestion. The repeatability of the procedure was checked in three experiments accomplished at weekly intervals. Clear distinction of the strains was achieved by cluster analysis. The differentiation of B. subtilis ecotypes was also improved significantly by the digestion method. The discriminatory power of the novel method was supported by an increase in the number of strain-specific peaks, as compared to the standard method. CONCLUSIONS: The method modulates the discriminatory power of MALDI-TOF MS profiling. The differentiation of a set of S. aureus, S. haemolyticus and B. subtilis strains was improved significantly after microwave-accelerated tryptic digestion of the cellular material.
ER -
BALÁŽOVÁ, Tereza, Ondrej ŠEDO, Polonca ŠTEFANIC, Ines MANDIC-MULEC, Michiel VOS and Zbyněk ZDRÁHAL. Improvement in Staphylococcus and Bacillus strain differentiation by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry profiling by using microwave-assisted enzymatic digestion. \textit{Rapid Communications in Mass Spectrometry}. Hoboken (USA): Wiley-Blackwell, 2014, vol.~28, No~17, p.~1855-1861. ISSN~0951-4198. Available from: https://dx.doi.org/10.1002/rcm.6966.
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