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@proceedings{1211802,
author = {Škorpíková, Lucie and Koudela, Břetislav and Ilgová, Jana and Šrámová, Eliška and Gelnar, Milan and Kašný, Martin},
booktitle = {XVI. Meeting of Biochemists and Molecular Biologists},
keywords = {Multi cystatin-like domain gene; Trichinella spiralis; Trichinella britovi; Trichinella pseudospiralis},
language = {eng},
title = {Cystatins of Trichinella spiralis, T. britovi and T. pseudospiralis},
year = {2014}
}
TY - CONF
ID - 1211802
AU - Škorpíková, Lucie - Koudela, Břetislav - Ilgová, Jana - Šrámová, Eliška - Gelnar, Milan - Kašný, Martin
PY - 2014
TI - Cystatins of Trichinella spiralis, T. britovi and T. pseudospiralis
KW - Multi cystatin-like domain gene
KW - Trichinella spiralis
KW - Trichinella britovi
KW - Trichinella pseudospiralis
N2 - The nematodes of the genus Trichinella are intracellular parasites which invade the cells of small intestine and skeletal muscles. They infect a broad range of worldwide distributed hosts, like mammals, birds and reptiles. All Trichinella spp. are zoonotic; six (T1, T2, T3, T4, T5 and T7) of twelve genotypes (T1-T12) have been detected in humans. The serious human disease is called trichinellosis and it has been documented in 55 countries. Our work is focused on cystatins - a reversible inhibitors of cystein peptidases of three trichinella species - Trichinella spiralis, T. britovi and T. pseudospiralis. Cystatins are compounds of excretory-secretory products (ESP) which are released by muscle larvae (L1) into the external environment. They are involved in many essential biological processes like growth, development, digestion and migration and also in manipulation of the host immunne system by parasite. Up to now, the specific primers were designed for “multi cystatin-like domain” gene of T. spiralis, T. britovi and T. pseudospiralis; this gene was amplified, cloned and sequenced. The comparison of sequential data showed that cystatin genes of T. spiralis and T. britovi are significantly homologous but differs to gene conserved motifs of T. pseudospiralis cystatin and in number of domains. The recombinant forms of cystatins of mentioned trichinella species will be prepared and their biochemical properties will be compared.
ER -
ŠKORPÍKOVÁ, Lucie, Břetislav KOUDELA, Jana ILGOVÁ, Eliška ŠRÁMOVÁ, Milan GELNAR a Martin KAŠNÝ. Cystatins of Trichinella spiralis, T. britovi and T. pseudospiralis. In \textit{XVI. Meeting of Biochemists and Molecular Biologists}. 2014.
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