Cystatins of the parasitic Nematodes - Trichinella spiralis, T.
britovi and T. pseudospiralis

k 2014

Cystatins of the parasitic Nematodes - Trichinella spiralis, T. britovi and T. pseudospiralis

ŠKORPÍKOVÁ, Lucie, Břetislav KOUDELA, Jana ILGOVÁ, Eliška ŠRÁMOVÁ, Milan GELNAR et. al.

Basic information

Original name

Cystatins of the parasitic Nematodes - Trichinella spiralis, T. britovi and T. pseudospiralis

Authors

ŠKORPÍKOVÁ, Lucie, Břetislav KOUDELA, Jana ILGOVÁ, Eliška ŠRÁMOVÁ, Milan GELNAR and Martin KAŠNÝ

Edition

48th Annual Meeting of the Austrian Society of Tropical Medicine, Parasitology and Migration Medicine, 2014

Other information

Language

English

Type of outcome

Prezentace na konferencích

Field of Study

10600 1.6 Biological sciences

Country of publisher

Austria

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Science

Keywords in English

Trichinella spiralis; Trichinella britovi; Trichinella pseudospiralis; Multi cystatin-like domain gene (mcd-1)

Změněno: 16/10/2015 10:00, Mgr. Lucie Škorpíková, Ph.D.

Abstract

V originále

The nematodes of the genus Trichinella are intracellular parasites which invade the cells of small intestine and skeletal muscles. They infect a broad range of worldwide distributed hosts, like mammals, birds and reptiles. All Trichinella spp. are zoonotic; six of twelve genotypes have been detected in humans. The serious human disease is called trichinellosis and it has been documented in 55 countries. Our work is focused on cystatins - a reversible inhibitors of cystein peptidases of three trichinella species - Trichinella spiralis, T. britovi and T. pseudospiralis. Cystatins are compounds of excretory-secretory products (ESP) which are released by muscle larvae (L1) into the external environment. They are involved in many essential biological processes like growth, development, digestion and migration and also in manipulation of the host immunne system by parasite. Up to now, the specific primers were designed for “multi cystatin-like domain” gene of T. spiralis, T. britovi and T. pseudospiralis; this gene was amplified, cloned and sequenced. The comparison of sequential data showed that cystatin genes of T. spiralis and T. britovi are significantly homologous but differs to gene conserved motifs of T. pseudospiralis cystatin and in number of domains. The recombinant forms of cystatins of mentioned trichinella species will be prepared and their biochemical properties will be compared.

Links

MUNI/A/0888/2013, interní kód MU

Name: Biodiverzita - analýzy biologických systémů různých úrovní a na různých škálách prostředí (Acronym: BIDA3)

Investor: Masaryk University, Category A

Citovat

ŠKORPÍKOVÁ, Lucie, Břetislav KOUDELA, Jana ILGOVÁ, Eliška ŠRÁMOVÁ, Milan GELNAR and Martin KAŠNÝ. Cystatins of the parasitic Nematodes - Trichinella spiralis, T. britovi and T. pseudospiralis. In 48th Annual Meeting of the Austrian Society of Tropical Medicine, Parasitology and Migration Medicine. 2014.

@proceedings{1211805,
   author = {Škorpíková, Lucie and Koudela, Břetislav and Ilgová, Jana and Šrámová, Eliška and Gelnar, Milan and Kašný, Martin},
   booktitle = {48th Annual Meeting of the Austrian Society of Tropical Medicine, Parasitology and Migration Medicine},
   keywords = {Trichinella spiralis; Trichinella britovi; Trichinella pseudospiralis; Multi cystatin-like domain gene (mcd-1)},
   language = {eng},
   title = {Cystatins of the parasitic Nematodes - Trichinella spiralis, T. britovi and T. pseudospiralis},
   year = {2014}
}

TY  - CONF
ID  - 1211805
AU  - Škorpíková, Lucie - Koudela, Břetislav - Ilgová, Jana - Šrámová, Eliška - Gelnar, Milan - Kašný, Martin
PY  - 2014
TI  - Cystatins of the parasitic Nematodes - Trichinella spiralis, T. britovi and T. pseudospiralis
KW  - Trichinella spiralis
KW  - Trichinella britovi
KW  - Trichinella pseudospiralis
KW  - Multi cystatin-like domain gene (mcd-1)
N2  - The nematodes of the genus Trichinella are intracellular parasites which invade the cells of small intestine and skeletal muscles. They infect a broad range of worldwide distributed hosts, like mammals, birds and reptiles. All Trichinella spp. are zoonotic; six of twelve genotypes have been detected in humans. The serious human disease is called trichinellosis and it has been documented in 55 countries. Our work is focused on cystatins - a reversible inhibitors of cystein peptidases of three trichinella species - Trichinella spiralis, T. britovi and T. pseudospiralis. Cystatins are compounds of excretory-secretory products (ESP) which are released by muscle larvae (L1) into the external environment. They are involved in many essential biological processes like growth, development, digestion and migration and also in manipulation of the host immunne system by parasite. Up to now, the specific primers were designed for “multi cystatin-like domain” gene of T. spiralis, T. britovi and T. pseudospiralis; this gene was amplified, cloned and sequenced. The comparison of sequential data showed that cystatin genes of T. spiralis and T. britovi are significantly homologous but differs to gene conserved motifs of T. pseudospiralis cystatin and in number of domains. The recombinant forms of cystatins of mentioned trichinella species will be prepared and their biochemical properties will be compared.
ER  -

ŠKORPÍKOVÁ, Lucie, Břetislav KOUDELA, Jana ILGOVÁ, Eliška ŠRÁMOVÁ, Milan GELNAR and Martin KAŠNÝ. Cystatins of the parasitic Nematodes - Trichinella spiralis, T. britovi and T. pseudospiralis. In \textit{48th Annual Meeting of the Austrian Society of Tropical Medicine, Parasitology and Migration Medicine}. 2014.

Detailed Information on Publication Record