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@article{1212414,
author = {Pelcová, Marta and Langmajerová, Monika and Cvingráfová, Eliška and Juřica, Jan and Glatz, Zdeněk},
article_number = {19},
doi = {http://dx.doi.org/10.1002/jssc.201400582},
keywords = {Dextromethorphan; Dextrorphan; Methanol; Nonaqueous media},
language = {eng},
issn = {1615-9306},
journal = {JOURNAL OF SEPARATION SCIENCE},
title = {Nonaqueous capillary electrophoresis of dextromethorphan and its metabolites},
volume = {37},
year = {2014}
}
TY - JOUR
ID - 1212414
AU - Pelcová, Marta - Langmajerová, Monika - Cvingráfová, Eliška - Juřica, Jan - Glatz, Zdeněk
PY - 2014
TI - Nonaqueous capillary electrophoresis of dextromethorphan and its metabolites
JF - JOURNAL OF SEPARATION SCIENCE
VL - 37
IS - 19
SP - 2785-2790
EP - 2785-2790
SN - 16159306
KW - Dextromethorphan
KW - Dextrorphan
KW - Methanol
KW - Nonaqueous media
N2 - This study deals with the nonaqueous capillary electrophoretic separation of dextromethorphan and its metabolites using a methanolic background electrolyte. The optimization of separation conditions was performed in terms of the resolution of dextromethorphan and dextrorphan and the effect of separation temperature, voltage, and the characteristics of the background electrolyte were studied. Complete separation of all analytes was achieved in 40 mM ammonium acetate dissolved in methanol. Hydrodynamic injection was performed at 3 kPa for 4 s. The separation voltage was 20 kV accompanied by a low electric current. The ultraviolet detection was performed at 214 nm, the temperature of the capillary was 25°C. These conditions enabled the separation of four analytes plus the internal standard within 9 min. Further, the developed method was validated in terms of linearity, sensitivity, and repeatability. Rat liver perfusate samples were subjected to the nonaqueous capillary electrophoretic method to illustrate its applicability.
ER -
PELCOVÁ, Marta, Monika LANGMAJEROVÁ, Eliška CVINGRÁFOVÁ, Jan JUŘICA a Zdeněk GLATZ. Nonaqueous capillary electrophoresis of dextromethorphan and its metabolites. Online. \textit{JOURNAL OF SEPARATION SCIENCE}. 2014, roč.~37, č.~19, s.~2785-2790. ISSN~1615-9306. Dostupné z: https://dx.doi.org/10.1002/jssc.201400582. [citováno 2024-04-24]
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