Nonaqueous capillary electrophoresis of dextromethorphan and its metabolites

PELCOVÁ, Marta, Monika LANGMAJEROVÁ, Eliška CVINGRÁFOVÁ, Jan JUŘICA and Zdeněk GLATZ. Nonaqueous capillary electrophoresis of dextromethorphan and its metabolites. JOURNAL OF SEPARATION SCIENCE. 2014, vol. 37, No 19, p. 2785-2790. ISSN 1615-9306. Available from: https://dx.doi.org/10.1002/jssc.201400582.

Basic information

• Original name: Nonaqueous capillary electrophoresis of dextromethorphan and its metabolites
• Authors: PELCOVÁ, Marta (203 Czech Republic, belonging to the institution), Monika LANGMAJEROVÁ (203 Czech Republic, belonging to the institution), Eliška CVINGRÁFOVÁ (203 Czech Republic, belonging to the institution), Jan JUŘICA (203 Czech Republic, belonging to the institution) and Zdeněk GLATZ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: JOURNAL OF SEPARATION SCIENCE, 2014, 1615-9306.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Germany
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 2.737
• RIV identification code: RIV/00216224:14310/14:00074172
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1002/jssc.201400582
• UT WoS: 000342909300021
• Keywords in English: Dextromethorphan; Dextrorphan; Methanol; Nonaqueous media
• Tags: AKR, rivok

Abstract

This study deals with the nonaqueous capillary electrophoretic separation of dextromethorphan and its metabolites using a methanolic background electrolyte. The optimization of separation conditions was performed in terms of the resolution of dextromethorphan and dextrorphan and the effect of separation temperature, voltage, and the characteristics of the background electrolyte were studied. Complete separation of all analytes was achieved in 40 mM ammonium acetate dissolved in methanol. Hydrodynamic injection was performed at 3 kPa for 4 s. The separation voltage was 20 kV accompanied by a low electric current. The ultraviolet detection was performed at 214 nm, the temperature of the capillary was 25°C. These conditions enabled the separation of four analytes plus the internal standard within 9 min. Further, the developed method was validated in terms of linearity, sensitivity, and repeatability. Rat liver perfusate samples were subjected to the nonaqueous capillary electrophoretic method to illustrate its applicability.

Links

• GBP206/12/G014, research and development project: Name: Centrum pokročilých bioanalytických technologií