VOLFOVÁ, Pavlína, Martina LENGEROVÁ, Jana LOCHMANOVÁ, Dana DVOŘÁKOVÁ, Dita ŘÍČNÁ, Martina PALACKOVÁ, Barbora WEINBERGEROVÁ, Jiří MAYER and Zdeněk RÁČIL. Detecting human cytomegalovirus drug resistant mutations and monitoring the emergence of resistant strains using real-time PCR. Journal of Clinical Virology. Amsterodam: Elsevier Science Inc, 2014, vol. 61, No 2, p. 270-274. ISSN 1386-6532. doi:10.1016/j.jcv.2014.07.008.
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Basic information
Original name Detecting human cytomegalovirus drug resistant mutations and monitoring the emergence of resistant strains using real-time PCR
Authors VOLFOVÁ, Pavlína (203 Czech Republic), Martina LENGEROVÁ (203 Czech Republic, guarantor, belonging to the institution), Jana LOCHMANOVÁ (203 Czech Republic), Dana DVOŘÁKOVÁ (203 Czech Republic, belonging to the institution), Dita ŘÍČNÁ (203 Czech Republic), Martina PALACKOVÁ (703 Slovakia), Barbora WEINBERGEROVÁ (203 Czech Republic), Jiří MAYER (203 Czech Republic, belonging to the institution) and Zdeněk RÁČIL (203 Czech Republic, belonging to the institution).
Edition Journal of Clinical Virology, Amsterodam, Elsevier Science Inc, 2014, 1386-6532.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30200 3.2 Clinical medicine
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 3.016
RIV identification code RIV/00216224:14740/14:00077729
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1016/j.jcv.2014.07.008
UT WoS 000342052300016
Keywords in English HCMV resistance; Real-time PCR; Sensitive/resistant strain; Repopulation
Tags EL OK, kontrola MP, ok, podil, rivok
Tags International impact, Reviewed
Changed by Changed by: Olga Křížová, učo 56639. Changed: 20. 1. 2015 05:57.
BACKGROUND: Antiviral resistance development is a serious complication of human cytomegalovirus virostatic therapy caused by mutations in UL 97 and/or UL54 genes. OBJECTIVES: To determinate the presence of sensitive and resistant strains in patients developing antiviral resistance. STUDY DESIGN: We used three different molecular biological methods for mutation analysis-restriction fragment length polymorphism, sequencing and real-time PCR approach. RESULTS: We describe three allogeneic hematopoietic stem cell transplant patients developing the GCV resistant HCMV strains manifested by virostatic treatment failure. In these patients we identified UL97 mutations L595S, A594V and A594T and monitored the dynamics of coexisted sensitive/resistant strains. We confirmed the presence of mixed HCMV populations and in two patients a phenomenon of sensitive strain repopulation which occurred after 6.5 months and 1 month after removing GCV pressure. CONCLUSIONS: Our results show changes in proportions of sensitive/resistant subpopulations over time but other studies would be required to demonstrate the beneficial impact of their monitoring on clinical outcome.
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