| VOLFOVÁ, Pavlína, Martina LENGEROVÁ, Jana LOCHMANOVÁ, Dana DVOŘÁKOVÁ, Dita ŘÍČNÁ, Martina PALACKOVÁ, Barbora WEINBERGEROVÁ, Jiří MAYER and Zdeněk RÁČIL. Detecting human cytomegalovirus drug resistant mutations and monitoring the emergence of resistant strains using real-time PCR. Journal of Clinical Virology. Amsterodam: Elsevier Science Inc, 2014, vol. 61, No 2, p. 270-274. ISSN 1386-6532. doi:10.1016/j.jcv.2014.07.008. |
Other formats:
BibTeX
LaTeX
RIS
@article{1212635,
author = {Volfová, Pavlína and Lengerová, Martina and Lochmanová, Jana and Dvořáková, Dana and Říčná, Dita and Palacková, Martina and Weinbergerová, Barbora and Mayer, Jiří and Ráčil, Zdeněk},
article_location = {Amsterodam},
article_number = {2},
doi = {http://dx.doi.org/10.1016/j.jcv.2014.07.008},
keywords = {HCMV resistance; Real-time PCR; Sensitive/resistant strain; Repopulation},
language = {eng},
issn = {1386-6532},
journal = {Journal of Clinical Virology},
title = {Detecting human cytomegalovirus drug resistant mutations and monitoring the emergence of resistant strains using real-time PCR},
url = {http://www.sciencedirect.com/science/article/pii/S1386653214002716},
volume = {61},
year = {2014}
}
TY - JOUR
ID - 1212635
AU - Volfová, Pavlína - Lengerová, Martina - Lochmanová, Jana - Dvořáková, Dana - Říčná, Dita - Palacková, Martina - Weinbergerová, Barbora - Mayer, Jiří - Ráčil, Zdeněk
PY - 2014
TI - Detecting human cytomegalovirus drug resistant mutations and monitoring the emergence of resistant strains using real-time PCR
JF - Journal of Clinical Virology
VL - 61
IS - 2
SP - 270-274
EP - 270-274
PB - Elsevier Science Inc
SN - 13866532
KW - HCMV resistance
KW - Real-time PCR
KW - Sensitive/resistant strain
KW - Repopulation
UR - http://www.sciencedirect.com/science/article/pii/S1386653214002716
L2 - http://www.sciencedirect.com/science/article/pii/S1386653214002716
N2 - BACKGROUND: Antiviral resistance development is a serious complication of human cytomegalovirus virostatic therapy caused by mutations in UL 97 and/or UL54 genes. OBJECTIVES: To determinate the presence of sensitive and resistant strains in patients developing antiviral resistance. STUDY DESIGN: We used three different molecular biological methods for mutation analysis-restriction fragment length polymorphism, sequencing and real-time PCR approach. RESULTS: We describe three allogeneic hematopoietic stem cell transplant patients developing the GCV resistant HCMV strains manifested by virostatic treatment failure. In these patients we identified UL97 mutations L595S, A594V and A594T and monitored the dynamics of coexisted sensitive/resistant strains. We confirmed the presence of mixed HCMV populations and in two patients a phenomenon of sensitive strain repopulation which occurred after 6.5 months and 1 month after removing GCV pressure. CONCLUSIONS: Our results show changes in proportions of sensitive/resistant subpopulations over time but other studies would be required to demonstrate the beneficial impact of their monitoring on clinical outcome.
ER -
VOLFOVÁ, Pavlína, Martina LENGEROVÁ, Jana LOCHMANOVÁ, Dana DVOŘÁKOVÁ, Dita ŘÍČNÁ, Martina PALACKOVÁ, Barbora WEINBERGEROVÁ, Jiří MAYER and Zdeněk RÁČIL. Detecting human cytomegalovirus drug resistant mutations and monitoring the emergence of resistant strains using real-time PCR. \textit{Journal of Clinical Virology}. Amsterodam: Elsevier Science Inc, 2014, vol.~61, No~2, p.~270-274. ISSN~1386-6532. doi:10.1016/j.jcv.2014.07.008.
|
