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@proceedings{1213303,
author = {Celá, Andrea and Dědová, Tereza and Mádr, Aleš and Glatz, Zdeněk},
booktitle = {CEITEC Annual Conference: "Frontiers in Material and Life Sciences"},
keywords = {capillary electrophoresis, fluorescence detection, on-capillary derivatization, amino acids},
language = {eng},
isbn = {978-80-210-7159-9},
title = {Nano scale derivatization followed by capillary electrophoresis with fluorescence detection},
year = {2014}
}
TY - CONF
ID - 1213303
AU - Celá, Andrea - Dědová, Tereza - Mádr, Aleš - Glatz, Zdeněk
PY - 2014
TI - Nano scale derivatization followed by capillary electrophoresis with fluorescence detection
SN - 9788021071599
KW - capillary electrophoresis, fluorescence detection, on-capillary derivatization, amino acids
N2 - Derivatization reaction allows modifying structure of originally non fluorescent compounds in order to detect original compounds by fluorescence detection. Such compounds could be amino acids, which play very important role in living organisms. Derivatization reagents are in general expensive and derivatization procedure is often time-consuming, laborious, and the products are instable. Narrow capillary can be also used as a reaction chamber of nano scale dimensions allowing automation of derivatization reaction minimizing manual steps, consumption of samples and reagents. Method development of on capillary derivatization reaction followed directly by capillary electrophoresis coupled with fluorescence detection of amino acids as well as optimization of separation conditions for analysis the reaction products are the subjects of this contribution.
ER -
CELÁ, Andrea, Tereza DĚDOVÁ, Aleš MÁDR and Zdeněk GLATZ. Nano scale derivatization followed by capillary electrophoresis with fluorescence detection. In \textit{CEITEC Annual Conference: ''Frontiers in Material and Life Sciences''}. 2014. ISBN~978-80-210-7159-9.
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