| MORIUCHI, Ryota, Hiroki TANAKA, Yuki NIKAWADORI, Mayuko ISHITSUKA, Michihiro ITO, Yoshiyuki OHTSUBO, Masataka TSUDA, Jiří DAMBORSKÝ, Zbyněk PROKOP and Yuji NAGATA. Stepwise Enhancement of Catalytic Performance of Haloalkane Dehalogenase LinB towards Beta-Hexachlorocyclohexane. Applied Microbiology and Biotechnology Express. London: Springer, 2014, vol. 4, No 72, p. 1-10. ISSN 2191-0855. Available from: https://dx.doi.org/10.1186/s13568-014-0072-5. |
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@article{1213423,
author = {Moriuchi, Ryota and Tanaka, Hiroki and Nikawadori, Yuki and Ishitsuka, Mayuko and Ito, Michihiro and Ohtsubo, Yoshiyuki and Tsuda, Masataka and Damborský, Jiří and Prokop, Zbyněk and Nagata, Yuji},
article_location = {London},
article_number = {72},
doi = {http://dx.doi.org/10.1186/s13568-014-0072-5},
keywords = {beta -Hexachlorocyclohexane; Xenobiotics; Biodegradation; Haloalkane dehalogenase; Protein evolution},
language = {eng},
issn = {2191-0855},
journal = {Applied Microbiology and Biotechnology Express},
title = {Stepwise Enhancement of Catalytic Performance of Haloalkane Dehalogenase LinB towards Beta-Hexachlorocyclohexane.},
url = {https://amb-express.springeropen.com/track/pdf/10.1186/s13568-014-0072-5},
volume = {4},
year = {2014}
}
TY - JOUR
ID - 1213423
AU - Moriuchi, Ryota - Tanaka, Hiroki - Nikawadori, Yuki - Ishitsuka, Mayuko - Ito, Michihiro - Ohtsubo, Yoshiyuki - Tsuda, Masataka - Damborský, Jiří - Prokop, Zbyněk - Nagata, Yuji
PY - 2014
TI - Stepwise Enhancement of Catalytic Performance of Haloalkane Dehalogenase LinB towards Beta-Hexachlorocyclohexane.
JF - Applied Microbiology and Biotechnology Express
VL - 4
IS - 72
SP - 1-10
EP - 1-10
PB - Springer
SN - 21910855
KW - beta -Hexachlorocyclohexane
KW - Xenobiotics
KW - Biodegradation
KW - Haloalkane dehalogenase
KW - Protein evolution
UR - https://amb-express.springeropen.com/track/pdf/10.1186/s13568-014-0072-5
L2 - https://amb-express.springeropen.com/track/pdf/10.1186/s13568-014-0072-5
N2 - Two haloalkane dehalogenases, LinBUT and LinBMI, each with 296 amino acid (aa) residues, exhibit only seven amino acid residue differences between them, but LinBMI‘s catalytic performance towards beta-hexachlorocyclohexane (beta-HCH) is considerably higher than LinBUT‘s. To elucidate the molecular basis governing this difference, intermediate mutants between LinBUT and LinBMI were constructed and kinetically characterized. The activities of LinBUT-based mutants gradually increased by cumulative mutations into LinBUT, and the effects of the individual amino acid substitutions depended on combination with other mutations. These results indicated that LinBUT‘s b-HCH degradation activity can be enhanced in a stepwise manner by the accumulation of point mutations.
ER -
MORIUCHI, Ryota, Hiroki TANAKA, Yuki NIKAWADORI, Mayuko ISHITSUKA, Michihiro ITO, Yoshiyuki OHTSUBO, Masataka TSUDA, Jiří DAMBORSKÝ, Zbyněk PROKOP and Yuji NAGATA. Stepwise Enhancement of Catalytic Performance of Haloalkane Dehalogenase LinB towards Beta-Hexachlorocyclohexane. \textit{Applied Microbiology and Biotechnology Express}. London: Springer, 2014, vol.~4, No~72, p.~1-10. ISSN~2191-0855. Available from: https://dx.doi.org/10.1186/s13568-014-0072-5.
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