| DVOŘÁK, Pavel, Nagendra Prasad KURUMBANG, Jaroslav BENDL, Jan BREZOVSKÝ, Zbyněk PROKOP and Jiří DAMBORSKÝ. Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization. ChemBioChem. WEINHEIM: WILEY-VCH, 2014, vol. 15, No 13, p. 1891-1895. ISSN 1439-4227. Available from: https://dx.doi.org/10.1002/cbic.201402265. |
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@article{1213428,
author = {Dvořák, Pavel and Kurumbang, Nagendra Prasad and Bendl, Jaroslav and Brezovský, Jan and Prokop, Zbyněk and Damborský, Jiří},
article_location = {WEINHEIM},
article_number = {13},
doi = {http://dx.doi.org/10.1002/cbic.201402265},
keywords = {biocatalysis; engineered enzymes},
language = {eng},
issn = {1439-4227},
journal = {ChemBioChem},
title = {Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization.},
volume = {15},
year = {2014}
}
TY - JOUR
ID - 1213428
AU - Dvořák, Pavel - Kurumbang, Nagendra Prasad - Bendl, Jaroslav - Brezovský, Jan - Prokop, Zbyněk - Damborský, Jiří
PY - 2014
TI - Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization.
JF - ChemBioChem
VL - 15
IS - 13
SP - 1891-1895
EP - 1891-1895
PB - WILEY-VCH
SN - 14394227
KW - biocatalysis
KW - engineered enzymes
N2 - Multi-enzyme processes represent an important area of biocatalysis. Their efficiency can be enhanced by optimization of biocatalysts’ stoichiometry. Here we present a workflow for maximizing the efficiency of a three-enzyme system catalysing a five-step chemical conversion. Kinetic models of pathways featuring either wild-type or engineered enzymes were built and the enzyme stoichiometry of each pathway was optimized. Mathematical modelling and one-pot multi-enzyme laboratory experiments provided detailed insights into pathway dynamics, enabled the selection of suitable engineered enzyme and afforded high efficiency while minimizing biocatalyst loadings. The optimizing of stoichiometry in a pathway with engineered enzyme reduced the total biocatalyst load by an impressive 56 %. Our new workflow represents a broadly applicable strategy for optimizing multi-enzyme processes.
ER -
DVOŘÁK, Pavel, Nagendra Prasad KURUMBANG, Jaroslav BENDL, Jan BREZOVSKÝ, Zbyněk PROKOP and Jiří DAMBORSKÝ. Maximizing the Efficiency of Multi-enzyme Process by Stoichiometry Optimization. \textit{ChemBioChem}. WEINHEIM: WILEY-VCH, 2014, vol.~15, No~13, p.~1891-1895. ISSN~1439-4227. Available from: https://dx.doi.org/10.1002/cbic.201402265.
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