Immobilized Synthetic Pathway for Biodegradation of Toxic
Recalcitrant Pollutant 1,2,3-Trichloropropane.

J 2014

Immobilized Synthetic Pathway for Biodegradation of Toxic Recalcitrant Pollutant 1,2,3-Trichloropropane.

DVOŘÁK, Pavel, Šárka BIDMANOVÁ, Jiří DAMBORSKÝ and Zbyněk PROKOP

Basic information

Original name

Immobilized Synthetic Pathway for Biodegradation of Toxic Recalcitrant Pollutant 1,2,3-Trichloropropane.

Authors

DVOŘÁK, Pavel (203 Czech Republic, belonging to the institution), Šárka BIDMANOVÁ (203 Czech Republic, belonging to the institution), Jiří DAMBORSKÝ (203 Czech Republic, guarantor, belonging to the institution) and Zbyněk PROKOP (203 Czech Republic, belonging to the institution)

Edition

Environmental Science & Technology, 2014, 0013-936X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 5.330

RIV identification code

RIV/00216224:14310/14:00074204

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1021/es500396r

UT WoS

000337646000041

Keywords in English

1;2;3-trichloropropane; engineered haloalkane dehalogenase; Rhodococcus rhodochrous;

Abstract

V originále

The anthropogenic compound 1,2,3-trichloropropane (TCP) has recently drawn attention as an emerging groundwater contaminant. No living organism, natural or engineered, is capable of the efficient aerobic utilization of this toxic industrial waste product. We describe a novel biotechnology for transforming TCP based on an immobilized synthetic pathway. The pathway is composed of three enzymes from two different microorganisms: engineered haloalkane dehalogenase from Rhodococcus rhodochrous NCIMB 13064, and haloalcohol dehalogenase and epoxide hydrolase from Agrobacterium radiobacter AD1. Together, they catalyze consecutive reactions converting toxic TCP to harmless glycerol. The pathway was immobilized in the form of purified enzymes or cell-free extracts, and its performance was tested in batch and continuous systems. Using a packed bed reactor filled with the immobilized biocatalyst, 52.6 millimoles of TCP were continuously converted into glycerol within 2.5 months of operation. The efficiency of the TCP conversion to the intermediates was 97%, and the efficiency of conversion to the final product glycerol was 78% during the operational period. Immobilized biocatalysts are suitable for removing TCP from contaminated water up to a 10 mM solubility limit, which is an order of magnitude higher than the concentration tolerated by living microorganisms.

Links

EE2.3.20.0183, research and development project

Name: Centrum experimentální biomedicíny

GAP503/12/0572, research and development project

Name: Konstrukce syntetické metabolické dráhy pro degradaci důležitého environmentálního polutantu proteinovým a metabolickým inženýrstvím

Investor: Czech Science Foundation

Citovat

DVOŘÁK, Pavel, Šárka BIDMANOVÁ, Jiří DAMBORSKÝ and Zbyněk PROKOP. Immobilized Synthetic Pathway for Biodegradation of Toxic Recalcitrant Pollutant 1,2,3-Trichloropropane. Environmental Science & Technology. 2014, vol. 48, No 12, p. 6859-6866. ISSN 0013-936X. Available from: https://dx.doi.org/10.1021/es500396r.

@article{1213449,
   author = {Dvořák, Pavel and Bidmanová, Šárka and Damborský, Jiří and Prokop, Zbyněk},
   article_number = {12},
   doi = {http://dx.doi.org/10.1021/es500396r},
   keywords = {1;2;3-trichloropropane; engineered haloalkane dehalogenase; Rhodococcus rhodochrous;},
   language = {eng},
   issn = {0013-936X},
   journal = {Environmental Science & Technology},
   title = {Immobilized Synthetic Pathway for Biodegradation of Toxic Recalcitrant Pollutant 1,2,3-Trichloropropane.},
   volume = {48},
   year = {2014}
}

TY  - JOUR
ID  - 1213449
AU  - Dvořák, Pavel - Bidmanová, Šárka - Damborský, Jiří - Prokop, Zbyněk
PY  - 2014
TI  - Immobilized Synthetic Pathway for Biodegradation of Toxic Recalcitrant Pollutant 1,2,3-Trichloropropane.
JF  - Environmental Science & Technology
VL  - 48
IS  - 12
SP  - 6859-6866
EP  - 6859-6866
SN  - 0013936X
KW  - 1;2;3-trichloropropane
KW  - engineered haloalkane dehalogenase
KW  - Rhodococcus rhodochrous;
N2  - The anthropogenic compound 1,2,3-trichloropropane (TCP) has recently drawn attention as an emerging groundwater contaminant. No living organism, natural or engineered, is capable of the efficient aerobic utilization of this toxic industrial waste product. We describe a novel biotechnology for transforming TCP based on an immobilized synthetic pathway. The pathway is composed of three enzymes from two different microorganisms: engineered haloalkane dehalogenase from Rhodococcus rhodochrous NCIMB 13064, and haloalcohol dehalogenase and epoxide hydrolase from Agrobacterium radiobacter AD1. Together, they catalyze consecutive reactions converting toxic TCP to harmless glycerol. The pathway was immobilized in the form of purified enzymes or cell-free extracts, and its performance was tested in batch and continuous systems. Using a packed bed reactor filled with the immobilized biocatalyst, 52.6 millimoles of TCP were continuously converted into glycerol within 2.5 months of operation. The efficiency of the TCP conversion to the intermediates was 97%, and the efficiency of conversion to the final product glycerol was 78% during the operational period. Immobilized biocatalysts are suitable for removing TCP from contaminated water up to a 10 mM solubility limit, which is an order of magnitude higher than the concentration tolerated by living microorganisms.
ER  -

DVOŘÁK, Pavel, Šárka BIDMANOVÁ, Jiří DAMBORSKÝ and Zbyněk PROKOP. Immobilized Synthetic Pathway for Biodegradation of Toxic Recalcitrant Pollutant 1,2,3-Trichloropropane. \textit{Environmental Science \&{} Technology}. 2014, vol.~48, No~12, p.~6859-6866. ISSN~0013-936X. Available from: https://dx.doi.org/10.1021/es500396r.

Detailed Information on Publication Record