Rapid Detection of Microorganisms Based on Active and Passive
Modes of QCM

J 2015

Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM

FARKA, Zdeněk, David KOVÁŘ and Petr SKLÁDAL

Basic information

Original name

Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM

Authors

FARKA, Zdeněk (203 Czech Republic, belonging to the institution), David KOVÁŘ (203 Czech Republic, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Sensors, Basel, (Switzerland), MDPI AG, 2015, 1424-8220

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.033

RIV identification code

RIV/00216224:14740/15:00082200

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.3390/s150100079

UT WoS

000348309400005

Keywords in English

Escherichia coli; immunosensor; impedance analysis; label-free detection; quartz crystal microbalance

Abstract

V originále

Label-free immunosensors are well suited for detection of microorganisms because of their fast response and reasonable sensitivity comparable to infection doses of common pathogens. Active (lever oscillator and frequency counter) and passive (impedance analyzer) modes of quartz crystal microbalance (QCM) were used and compared for rapid detection of three strains of E. coli. Different approaches for antibody immobilization were compared, the immobilization of reduced antibody using Sulfo-SMCC was most effective achieving the limit of detection (LOD) 8 × 10^4 CFU·mL-1 in 10 min. For the passive mode, software evaluating impedance characteristics in real-time was developed and used. Almost the same results were achieved using both active and passive modes confirming that the sensor properties are not limited by the frequency evaluation method but mainly by affinity of the antibody. Furthermore, reference measurements were done using surface plasmon resonance. Effect of condition of cells on signal was observed showing that cells ruptured by ultrasonication provided slightly higher signal changes than intact microbes.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

Citovat

FARKA, Zdeněk, David KOVÁŘ and Petr SKLÁDAL. Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM. Sensors. Basel, (Switzerland): MDPI AG, 2015, vol. 15, No 1, p. 79-92. ISSN 1424-8220. Available from: https://dx.doi.org/10.3390/s150100079.

@article{1214396,
   author = {Farka, Zdeněk and Kovář, David and Skládal, Petr},
   article_location = {Basel, (Switzerland)},
   article_number = {1},
   doi = {http://dx.doi.org/10.3390/s150100079},
   keywords = {Escherichia coli; immunosensor; impedance analysis; label-free detection; quartz crystal microbalance},
   language = {eng},
   issn = {1424-8220},
   journal = {Sensors},
   title = {Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM},
   url = {http://www.mdpi.com/1424-8220/15/1/79},
   volume = {15},
   year = {2015}
}

TY  - JOUR
ID  - 1214396
AU  - Farka, Zdeněk - Kovář, David - Skládal, Petr
PY  - 2015
TI  - Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM
JF  - Sensors
VL  - 15
IS  - 1
SP  - 79-92
EP  - 79-92
PB  - MDPI AG
SN  - 14248220
KW  - Escherichia coli
KW  - immunosensor
KW  - impedance analysis
KW  - label-free detection
KW  - quartz crystal microbalance
UR  - http://www.mdpi.com/1424-8220/15/1/79
L2  - http://www.mdpi.com/1424-8220/15/1/79
N2  - Label-free immunosensors are well suited for detection of microorganisms because of their fast response and reasonable sensitivity comparable to infection doses of common pathogens. Active (lever oscillator and frequency counter) and passive (impedance analyzer) modes of quartz crystal microbalance (QCM) were used and compared for rapid detection of three strains of E. coli. Different approaches for antibody immobilization were compared, the immobilization of reduced antibody using Sulfo-SMCC was most effective achieving the limit of detection (LOD) 8 × 10^4 CFU·mL-1 in 10 min. For the passive mode, software evaluating impedance characteristics in real-time was developed and used. Almost the same results were achieved using both active and passive modes confirming that the sensor properties are not limited by the frequency evaluation method but mainly by affinity of the antibody. Furthermore, reference measurements were done using surface plasmon resonance. Effect of condition of cells on signal was observed showing that cells ruptured by ultrasonication provided slightly higher signal changes than intact microbes.
ER  -

FARKA, Zdeněk, David KOVÁŘ and Petr SKLÁDAL. Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM. \textit{Sensors}. Basel, (Switzerland): MDPI AG, 2015, vol.~15, No~1, p.~79-92. ISSN~1424-8220. Available from: https://dx.doi.org/10.3390/s150100079.

Detailed Information on Publication Record