Detailed Information on Publication Record
2015
Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM
FARKA, Zdeněk, David KOVÁŘ and Petr SKLÁDALBasic information
Original name
Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM
Authors
FARKA, Zdeněk (203 Czech Republic, belonging to the institution), David KOVÁŘ (203 Czech Republic, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution)
Edition
Sensors, Basel, (Switzerland), MDPI AG, 2015, 1424-8220
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Switzerland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 2.033
RIV identification code
RIV/00216224:14740/15:00082200
Organization unit
Central European Institute of Technology
UT WoS
000348309400005
Keywords in English
Escherichia coli; immunosensor; impedance analysis; label-free detection; quartz crystal microbalance
Tags
Tags
International impact, Reviewed
Změněno: 28/9/2017 11:06, doc. Mgr. Zdeněk Farka, Ph.D.
Abstract
V originále
Label-free immunosensors are well suited for detection of microorganisms because of their fast response and reasonable sensitivity comparable to infection doses of common pathogens. Active (lever oscillator and frequency counter) and passive (impedance analyzer) modes of quartz crystal microbalance (QCM) were used and compared for rapid detection of three strains of E. coli. Different approaches for antibody immobilization were compared, the immobilization of reduced antibody using Sulfo-SMCC was most effective achieving the limit of detection (LOD) 8 × 10^4 CFU·mL-1 in 10 min. For the passive mode, software evaluating impedance characteristics in real-time was developed and used. Almost the same results were achieved using both active and passive modes confirming that the sensor properties are not limited by the frequency evaluation method but mainly by affinity of the antibody. Furthermore, reference measurements were done using surface plasmon resonance. Effect of condition of cells on signal was observed showing that cells ruptured by ultrasonication provided slightly higher signal changes than intact microbes.
Links
ED1.1.00/02.0068, research and development project |
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