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@proceedings{1214593, author = {Mrázková, Jana and Pokorná, Martina and Wimmerová, Michaela}, booktitle = {XVI. Setkání biochemiků a molekulárních biologů}, keywords = {lectins; PA-IIL; Pseudomonas aeruginosa; targeted-random mutagenesis; high-throughput screening; mutant library}, language = {eng}, isbn = {978-80-210-7340-1}, title = {Targeted-random mutagenesis of PA-IIL lectin and high-throughput screening of mutant library}, year = {2014} }
TY - CONF ID - 1214593 AU - Mrázková, Jana - Pokorná, Martina - Wimmerová, Michaela PY - 2014 TI - Targeted-random mutagenesis of PA-IIL lectin and high-throughput screening of mutant library SN - 9788021073401 KW - lectins KW - PA-IIL KW - Pseudomonas aeruginosa KW - targeted-random mutagenesis KW - high-throughput screening KW - mutant library N2 - PA-IIL lectin from Pseudomonas aeruginosa shows a unique binding mode among bacterial lectins because it binds saccharides through two calcium cations. Its features make this protein a good candidate for tuning its affinity and specificity. Two amino acids responsible for sugar binding were chosen for targeted-random mutagenesis. If this approach is used, it is necessary to utilize high-throughput screening of mutant libraries for identification of mutants with interestingly modified specificity and/or affinity. ER -
MRÁZKOVÁ, Jana, Martina POKORNÁ and Michaela WIMMEROVÁ. Targeted-random mutagenesis of PA-IIL lectin and high-throughput screening of mutant library. In \textit{XVI. Setkání biochemiků a molekulárních biologů}. 2014. ISBN~978-80-210-7340-1.
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