Acinetobacter bohemicus sp nov widespread in natural soil and
water ecosystems in the Czech Republic

J 2014

Acinetobacter bohemicus sp nov widespread in natural soil and water ecosystems in the Czech Republic

KŘÍŽOVÁ, Lenka, Martina MAIXNEROVÁ, Ondrej ŠEDO and Alexandr NEMEC

Basic information

Original name

Acinetobacter bohemicus sp nov widespread in natural soil and water ecosystems in the Czech Republic

Authors

KŘÍŽOVÁ, Lenka (203 Czech Republic), Martina MAIXNEROVÁ (203 Czech Republic), Ondrej ŠEDO (203 Czech Republic, guarantor, belonging to the institution) and Alexandr NEMEC (203 Czech Republic)

Edition

Systematic and Applied Microbiology, Jena (Germany), Urban & Fischer, 2014, 0723-2020

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.283

RIV identification code

RIV/00216224:14740/14:00074264

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.syapm.2014.07.001

UT WoS

000343635900001

Keywords in English

rpoB; gyrB; 16S rRNA gene; ANI; Carbon source assimilation; MALDI-TOF MS

Abstract

V originále

We investigated the taxonomic status of a phenetically unique group of 25 Acinetobacter strains which were isolated from multiple soil and water samples collected in natural ecosystems in the Czech Republic. Based on the comparative sequence analyses of the rpoB, gyrB, and 16S rRNA genes, the strains formed a coherent and well separated branch within the genus Acinetobacter. The genomic uniqueness of the group at the species level was supported by the low average nucleotide identity values (<= 77.37%) between the whole genome sequences of strain ANC 3994(T) (NCBI accession no. APOH00000000) and the representatives of the known Acinetobacter species. Moreover, all 25 strains created a tight cluster clearly separated from all hitherto described species based on whole-cell protein profiling by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and shared a unique combination of metabolic and physiological properties. The capacity to assimilate L-histidine and the inability to grow at 35 degrees C differentiated them from their phenotypically closest neighbor, Acinetobacter johnsonii. We conclude that the 25 strains represent a novel Acinetobacter species, for which the name Acinetobacter bohemicus sp. nov. is proposed. The type strain of A. bohemicus is ANC 3994(T) (=CIP 110496(T) = CCUG 63842(T) = CCM 8462(T)).

Links

GA13-26693S, research and development project

Name: Genetická diverzita a fylogeneze rodu Acinetobacter v přírodních ekosystémech.

Investor: Czech Science Foundation

Citovat

KŘÍŽOVÁ, Lenka, Martina MAIXNEROVÁ, Ondrej ŠEDO and Alexandr NEMEC. Acinetobacter bohemicus sp nov widespread in natural soil and water ecosystems in the Czech Republic. Systematic and Applied Microbiology. Jena (Germany): Urban & Fischer, 2014, vol. 37, No 7, p. 467-473. ISSN 0723-2020. Available from: https://dx.doi.org/10.1016/j.syapm.2014.07.001.

@article{1214919,
   author = {Křížová, Lenka and Maixnerová, Martina and Šedo, Ondrej and Nemec, Alexandr},
   article_location = {Jena (Germany)},
   article_number = {7},
   doi = {http://dx.doi.org/10.1016/j.syapm.2014.07.001},
   keywords = {rpoB; gyrB; 16S rRNA gene; ANI; Carbon source assimilation; MALDI-TOF MS},
   language = {eng},
   issn = {0723-2020},
   journal = {Systematic and Applied Microbiology},
   title = {Acinetobacter bohemicus sp nov widespread in natural soil and water ecosystems in the Czech Republic},
   url = {http://ac.els-cdn.com/S0723202014001015/1-s2.0-S0723202014001015-main.pdf?_tid=3acb56e0-c7dd-11e4-9ce5-00000aab0f01&acdnat=1426071551_9208211a79d54dbbf3b9f209899d12f7},
   volume = {37},
   year = {2014}
}

TY  - JOUR
ID  - 1214919
AU  - Křížová, Lenka - Maixnerová, Martina - Šedo, Ondrej - Nemec, Alexandr
PY  - 2014
TI  - Acinetobacter bohemicus sp nov widespread in natural soil and water ecosystems in the Czech Republic
JF  - Systematic and Applied Microbiology
VL  - 37
IS  - 7
SP  - 467-473
EP  - 467-473
PB  - Urban & Fischer
SN  - 07232020
KW  - rpoB
KW  - gyrB
KW  - 16S rRNA gene
KW  - ANI
KW  - Carbon source assimilation
KW  - MALDI-TOF MS
UR  - http://ac.els-cdn.com/S0723202014001015/1-s2.0-S0723202014001015-main.pdf?_tid=3acb56e0-c7dd-11e4-9ce5-00000aab0f01&acdnat=1426071551_9208211a79d54dbbf3b9f209899d12f7
L2  - http://ac.els-cdn.com/S0723202014001015/1-s2.0-S0723202014001015-main.pdf?_tid=3acb56e0-c7dd-11e4-9ce5-00000aab0f01&acdnat=1426071551_9208211a79d54dbbf3b9f209899d12f7
N2  - We investigated the taxonomic status of a phenetically unique group of 25 Acinetobacter strains which were isolated from multiple soil and water samples collected in natural ecosystems in the Czech Republic. Based on the comparative sequence analyses of the rpoB, gyrB, and 16S rRNA genes, the strains formed a coherent and well separated branch within the genus Acinetobacter. The genomic uniqueness of the group at the species level was supported by the low average nucleotide identity values (<= 77.37%) between the whole genome sequences of strain ANC 3994(T) (NCBI accession no. APOH00000000) and the representatives of the known Acinetobacter species. Moreover, all 25 strains created a tight cluster clearly separated from all hitherto described species based on whole-cell protein profiling by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and shared a unique combination of metabolic and physiological properties. The capacity to assimilate L-histidine and the inability to grow at 35 degrees C differentiated them from their phenotypically closest neighbor, Acinetobacter johnsonii. We conclude that the 25 strains represent a novel Acinetobacter species, for which the name Acinetobacter bohemicus sp. nov. is proposed. The type strain of A. bohemicus is ANC 3994(T) (=CIP 110496(T) = CCUG 63842(T) = CCM 8462(T)).
ER  -

KŘÍŽOVÁ, Lenka, Martina MAIXNEROVÁ, Ondrej ŠEDO and Alexandr NEMEC. Acinetobacter bohemicus sp nov widespread in natural soil and water ecosystems in the Czech Republic. \textit{Systematic and Applied Microbiology}. Jena (Germany): Urban \&{} Fischer, 2014, vol.~37, No~7, p.~467-473. ISSN~0723-2020. Available from: https://dx.doi.org/10.1016/j.syapm.2014.07.001.

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