BABINSKÝ, Martin, Radovan FIALA, Iva KEJNOVSKÁ, Klára BEDNÁŘOVÁ, Radek MAREK, Janos SAGI, Vladimír SKLENÁŘ and Michaela VORLÍČKOVÁ. Loss of loop adenines alters human telomere d(AG3(TTAG3)3) quadruplex folding. Nucleic Acids Research. UK: Oxford University Press, 2014, vol. 42, No 22, p. 14031-14041. ISSN 0305-1048. doi:10.1093/nar/gku1245.
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Basic information
Original name Loss of loop adenines alters human telomere d(AG3(TTAG3)3) quadruplex folding
Authors BABINSKÝ, Martin (703 Slovakia, belonging to the institution), Radovan FIALA (203 Czech Republic, belonging to the institution), Iva KEJNOVSKÁ (203 Czech Republic, belonging to the institution), Klára BEDNÁŘOVÁ (203 Czech Republic, belonging to the institution), Radek MAREK (203 Czech Republic, belonging to the institution), Janos SAGI (840 United States of America), Vladimír SKLENÁŘ (203 Czech Republic, guarantor, belonging to the institution) and Michaela VORLÍČKOVÁ (203 Czech Republic, belonging to the institution).
Edition Nucleic Acids Research, UK, Oxford University Press, 2014, 0305-1048.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW DOI: 10.1093/nar/gku1245
Impact factor Impact factor: 9.112
RIV identification code RIV/00216224:14740/14:00073361
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1093/nar/gku1245
UT WoS 000347916900057
Keywords in English Quadruplex DNA; abasic lesion; NMR; CD;
Tags kontrola MP, MP, OA, rivok
Tags International impact, Reviewed
Changed by Changed by: Martina Prášilová, učo 342282. Changed: 10. 3. 2015 14:23.
Abstract
Abasic (AP) lesions are the most frequent type of damages occurring in cellular DNA. Here we describe the conformational effects of AP sites substituted for 2'-deoxyadenosine in the first (ap7), second (ap13) or third (ap19) loop of the quadruplex formed in K+ by the human telomere DNA 5'-d[AG3(TTAG3)3]. CD spectra and electrophoresis reveal that the presence of AP sites does not hinder the formation of intramolecular quadruplexes. NMR spectra show that the structural heterogeneity is substantially reduced in ap7 and ap19 as compared to that in the wild-type. These two (ap7 and ap19) sequences are shown to adopt the hybrid-1 and hybrid-2 quadruplex topology, respectively, with AP site located in a propeller-like loop. All three studied sequences transform easily into parallel quadruplex in dehydrating ethanol solution. Thus, the AP site in any loop region facilitates the formation of the propeller loop. Substitution of all adenines by AP sites stabilizes the parallel quadruplex even in the absence of ethanol. Whereas guanines are the major determinants of quadruplex stability, the presence or absence of loop adenines substantially influences quadruplex folding. The naturally occurring adenine-lacking sites in the human telomere DNA can change the quadruplex topology in vivo with potentially vital biological consequences.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
EE2.3.20.0042, research and development projectName: Internacionalizace programu Strukturní biologie s důrazem na rozvoj nových směrů výzkumu
IAA500040903, research and development projectName: Biofyzika a bioinformatika genomových fragmentů DNA velice bohatých na guanin a adenin
Investor: Academy of Sciences of the Czech Republic, Biophysics and bioinformatics of genomic DNA fragments very rich in guanine and adenine
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