Dorsal root ganglia microvascularization in rat neuropathic
pain model

a 2014

Dorsal root ganglia microvascularization in rat neuropathic pain model

JOUKAL, Marek; Ondřej MAREK; Miloslav VLČEK; Ilona KLUSÁKOVÁ; Petr DUBOVÝ et. al.

Základní údaje

Originální název

Dorsal root ganglia microvascularization in rat neuropathic pain model

Název česky

Mikrovaskularizace spinálních ganglií v modelu neuropatické bolesti laboratorního potkana

Autoři

JOUKAL, Marek; Ondřej MAREK; Miloslav VLČEK; Ilona KLUSÁKOVÁ a Petr DUBOVÝ

Vydání

Morphology 2014, 2014

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Lékařská fakulta

Klíčová slova česky

spinální ganglion, mikrovaskularizace, neuropatická bolest

Klíčová slova anglicky

dorsal root ganglion, microvascularization, neuropathic pain

Příznaky

Mezinárodní význam

Změněno: 12. 1. 2015 09:11, doc. MUDr. Marek Joukal, Ph.D.

Anotace

V originále

The bodies of primary sensory neurons and satellite glial cells (SGC) form a unit which is surrounded by the blood vessels. The SGC and blood capillaries participate in maintenance of the microenvironment stability necessary for the normal functioning of the primary sensory neurons. Cellular and molecular changes occur in the DRG following peripheral nerve injury, including proliferation of SGC and capillaries. The aim of present experiments was to visualize the relation between primary sensory neuron-SGC unit and microvascularization in intact rats and neuropathic pain model using immunohistochemical staining and confocal microscope. Six Wistar rats (male, 250-300g) were anaesthetized with a mixture of ketamine and xylazine and unilateral chronic constriction injury (CCI) of the sciatic nerve was performed. CCI-operated rats were left to survive 21 days. Three naïve rats were used for control study. The rats were deeply anaesthetized, perfused transcardially by Zamboni´s fixative and L4-L5 DRG with surrounding tissue was removed. ScaleA2 technique was used for increase of tissue transparency. Immunohistochemical staining for RECA and GFAP was used for visualization of endothelia and activated satellite glial cells (SGCs), respectively. Double staining for GFAP and RECA showed activation of SGCs and vascular proliferation in both ipsilateral and contralateral DRG of CCI-operated animals. Capillary plexuses were densely distributed among SGC as well as between the neuronal bodies and SGC. The results evidenced that the blood vessels proliferated in both ipsilateral and contralateral DRG after unilateral CCI of the sciatic nerve. Microvascular network organized close to neuronal bodies may contribute to neuropathic pain development by systemic diffusion of inflammatory mediators not only in ipsilateral but also contralateral DRG.

Návaznosti

MUNI/A/0793/2013, interní kód MU

Název: Prostorová vizualizace vztahu arachnoidey, mikrovaskularizace a spinálních ganglií

Investor: Masarykova univerzita, Prostorová vizualizace vztahu arachnoidey, mikrovaskularizace a spinálních ganglií, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

JOUKAL, Marek; Ondřej MAREK; Miloslav VLČEK; Ilona KLUSÁKOVÁ a Petr DUBOVÝ. Dorsal root ganglia microvascularization in rat neuropathic pain model. In Morphology 2014. 2014.

@proceedings{1216079,
   author = {Joukal, Marek and Marek, Ondřej and Vlček, Miloslav and Klusáková, Ilona and Dubový, Petr},
   booktitle = {Morphology 2014},
   keywords = {dorsal root ganglion, microvascularization, neuropathic pain},
   language = {eng},
   title = {Dorsal root ganglia microvascularization in rat neuropathic pain model},
   year = {2014}
}

TY  - CONF
ID  - 1216079
AU  - Joukal, Marek - Marek, Ondřej - Vlček, Miloslav - Klusáková, Ilona - Dubový, Petr
PY  - 2014
TI  - Dorsal root ganglia microvascularization in rat neuropathic pain model
KW  - dorsal root ganglion, microvascularization, neuropathic pain
N2  - The bodies of primary sensory neurons and satellite glial cells (SGC) form a unit which is surrounded by the blood vessels. The SGC and blood capillaries participate in maintenance of the microenvironment stability necessary for the normal functioning of the primary sensory neurons. Cellular and molecular changes occur in the DRG following peripheral nerve injury, including proliferation of SGC and capillaries. The aim of present experiments was to visualize the relation between primary sensory neuron-SGC unit and microvascularization in intact rats and neuropathic pain model using immunohistochemical staining and confocal microscope. Six Wistar rats (male, 250-300g) were anaesthetized with a mixture of ketamine and xylazine and unilateral chronic constriction injury (CCI) of the sciatic nerve was performed. CCI-operated rats were left to survive 21 days. Three naïve rats were used for control study. The rats were deeply anaesthetized, perfused transcardially by Zamboni´s fixative and L4-L5 DRG with surrounding tissue was removed. ScaleA2 technique was used for increase of tissue transparency. Immunohistochemical staining for RECA and GFAP was used for visualization of endothelia and activated satellite glial cells (SGCs), respectively. Double staining for GFAP and RECA showed activation of SGCs and vascular proliferation in both ipsilateral and contralateral DRG of CCI-operated animals. Capillary plexuses were densely distributed among SGC as well as between the neuronal bodies and SGC. The results evidenced that the blood vessels proliferated in both ipsilateral and contralateral DRG after unilateral CCI of the sciatic nerve. Microvascular network organized close to neuronal bodies may contribute to neuropathic pain development by systemic diffusion of inflammatory mediators not only in ipsilateral but also contralateral DRG.
ER  -

JOUKAL, Marek; Ondřej MAREK; Miloslav VLČEK; Ilona KLUSÁKOVÁ a Petr DUBOVÝ. Dorsal root ganglia microvascularization in rat neuropathic pain model. In \textit{Morphology 2014}. 2014.

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