k 2014

MALDI-TOF fingerprinting of the whole mammalian cell-lines

AMATO, Filippo, Lukáš KUČERA, Petr VAŇHARA, Aleš HAMPL, Josef HAVEL et. al.

Basic information

Original name

MALDI-TOF fingerprinting of the whole mammalian cell-lines

Authors

AMATO, Filippo (380 Italy, belonging to the institution), Lukáš KUČERA (203 Czech Republic, belonging to the institution), Petr VAŇHARA (203 Czech Republic, belonging to the institution), Aleš HAMPL (203 Czech Republic, belonging to the institution) and Josef HAVEL (203 Czech Republic, guarantor, belonging to the institution)

Edition

OrganoNET conference 2014, 2014

Other information

Language

English

Type of outcome

Prezentace na konferencích

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/14:00078221

Organization unit

Faculty of Science

Keywords (in Czech)

mammalian cell-lines; MALDI-TOF; mass spectrometry; fingerprinting

Keywords in English

mammalian cell-lines; MALDI-TOF; mass spectrometry; fingerprinting

Tags

International impact, Reviewed
Změněno: 28/4/2015 09:29, Ing. Andrea Mikešková

Abstract

V originále

Robust cell-lines identification requires reliable databases of cell-lines fingerprints. Mass spectrometric fingerprinting of mammalian cell-lines is a promising way to achieve the goal of fast, cheap, simple and reliable cell-lines authentication. However, general and standardized method for mass spectrometric fingerprinting of the whole mammalian cell-lines is not available to date. We developed, optimized and validated MALDI-TOF mass spectrometric method for reliable, specific and reproducible whole-cell fingerprinting of mammalian cell-lines. Quality and robustness of mass spectrometric fingerprints is demonstrated by statistical analysis of experimental data. Developed method for the whole mammalian cell-lines fingerprinting, in contrast to actual procedures, is inexpensive, fast and it opens the door towards routine cell authentication.

Links

ED2.1.00/03.0086, research and development project
Name: Regionální VaV centrum pro nízkonákladové plazmové a nanotechnologické povrchové úpravy
MSM0021622411, plan (intention)
Name: Studium a aplikace plazmochemických reakcí v neizotermickém nízkoteplotním plazmatu a jeho interakcí s povrchem pevných látek
Investor: Ministry of Education, Youth and Sports of the CR, Study and application of plasma chemical reactions in non-isothermic low temperature plasma and its interaction with solid surface
MSM0021622430, plan (intention)
Name: Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie
Investor: Ministry of Education, Youth and Sports of the CR, Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies