| SMETANA, Jan, Jan FRÖHLICH, Romana ZAORALOVÁ, Vladimíra VALLOVÁ, Henrieta GREŠLIKOVÁ, Renata KUPSKÁ, Pavel NĚMEC, Aneta MIKULÁŠOVÁ, Martina ALMAŠI, Luděk POUR, Zdeněk ADAM, Viera SANDECKÁ, Lenka ZAHRADOVÁ, Roman HÁJEK a Petr KUGLÍK. Genome-Wide Screening of Cytogenetic Abnormalities in Multiple Myeloma Patients Using Array-CGH Technique: A Czech Multicenter Experience. BioMed Research International. Hindawi Publishing Corporation, 2014, Neuveden, MAY, s. "nestrankovano", 9 s. ISSN 2314-6133. Dostupné z: https://dx.doi.org/10.1155/2014/209670. |
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@article{1216373,
author = {Smetana, Jan and Fröhlich, Jan and Zaoralová, Romana and Vallová, Vladimíra and Grešliková, Henrieta and Kupská, Renata and Němec, Pavel and Mikulášová, Aneta and Almaši, Martina and Pour, Luděk and Adam, Zdeněk and Sandecká, Viera and Zahradová, Lenka and Hájek, Roman and Kuglík, Petr},
article_number = {MAY},
doi = {http://dx.doi.org/10.1155/2014/209670},
keywords = {Multiple myeloma; genome-wide profiling; array-CGH; cytogenetics; FISH},
language = {eng},
issn = {2314-6133},
journal = {BioMed Research International},
title = {Genome-Wide Screening of Cytogenetic Abnormalities in Multiple Myeloma Patients Using Array-CGH Technique: A Czech Multicenter Experience},
url = {http://www.hindawi.com/journals/bmri/2014/209670/},
volume = {Neuveden},
year = {2014}
}
TY - JOUR
ID - 1216373
AU - Smetana, Jan - Fröhlich, Jan - Zaoralová, Romana - Vallová, Vladimíra - Grešliková, Henrieta - Kupská, Renata - Němec, Pavel - Mikulášová, Aneta - Almaši, Martina - Pour, Luděk - Adam, Zdeněk - Sandecká, Viera - Zahradová, Lenka - Hájek, Roman - Kuglík, Petr
PY - 2014
TI - Genome-Wide Screening of Cytogenetic Abnormalities in Multiple Myeloma Patients Using Array-CGH Technique: A Czech Multicenter Experience
JF - BioMed Research International
VL - Neuveden
IS - MAY
SP - "nestrankovano"
EP - "nestrankovano"
PB - Hindawi Publishing Corporation
SN - 23146133
KW - Multiple myeloma
KW - genome-wide profiling
KW - array-CGH
KW - cytogenetics
KW - FISH
UR - http://www.hindawi.com/journals/bmri/2014/209670/
L2 - http://www.hindawi.com/journals/bmri/2014/209670/
N2 - Characteristic recurrent copy number aberrations (CNAs) play a key role in multiple myeloma (MM) pathogenesis and have important prognostic significance for MM patients. Array-based comparative genomic hybridization (aCGH) provides a powerful tool for genome-wide classification of CNAs and thus should be implemented into MM routine diagnostics. We demonstrate the possibility of effective utilization of oligonucleotide-based aCGH in 91 MM patients. Chromosomal aberrations associated with effect on the prognosis of MM were initially evaluated by I-FISH and were found in 93.4% (85/91). Incidence of hyperdiploidy was 49.5% (45/91); del(13)(q14) was detected in 57.1% (52/91); gain(1)(q21) occurred in 58.2% (53/91); del(17)(p13) was observed in 15.4% (14/91); and t(4;14)(p16;q32) was found in 18.6% (16/86). Genome-wide screening using Agilent 44K aCGH microarrays revealed copy number alterations in 100% (91/91). Most common deletions were found at 13q (58.9%), 1p (39.6%), and 8p (31.1%), whereas gain of whole 1q was the most often duplicated region (50.6%). Furthermore, frequent homozygous deletions of genes playing important role in myeloma biology such as TRAF3, BIRC1/BIRC2, RB1, or CDKN2C were observed. Taken together, we demonstrated the utilization of aCGH technique in clinical diagnostics as powerful tool for identification of unbalanced genomic abnormalities with prognostic significance for MM patients.
ER -
SMETANA, Jan, Jan FRÖHLICH, Romana ZAORALOVÁ, Vladimíra VALLOVÁ, Henrieta GREŠLIKOVÁ, Renata KUPSKÁ, Pavel NĚMEC, Aneta MIKULÁŠOVÁ, Martina ALMAŠI, Luděk POUR, Zdeněk ADAM, Viera SANDECKÁ, Lenka ZAHRADOVÁ, Roman HÁJEK a Petr KUGLÍK. Genome-Wide Screening of Cytogenetic Abnormalities in Multiple Myeloma Patients Using Array-CGH Technique: A Czech Multicenter Experience. \textit{BioMed Research International}. Hindawi Publishing Corporation, 2014, Neuveden, MAY, s.~''nestrankovano'', 9 s. ISSN~2314-6133. Dostupné z: https://dx.doi.org/10.1155/2014/209670.
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