miR-150 influences B-cell receptor signaling in chronic
lymphocytic leukemia by regulating expression of GAB1 and FOXP1

J 2014

miR-150 influences B-cell receptor signaling in chronic lymphocytic leukemia by regulating expression of GAB1 and FOXP1

MRÁZ, Marek; L.G. CHEN; L.Z. RASSENTI; E.M. GHIA; H.Y. LI et. al.

Základní údaje

Originální název

miR-150 influences B-cell receptor signaling in chronic lymphocytic leukemia by regulating expression of GAB1 and FOXP1

Autoři

MRÁZ, Marek ; L.G. CHEN; L.Z. RASSENTI; E.M. GHIA; H.Y. LI; K. JEPSEN; E.N. SMITH; K. MESSER; K.A. FRAZER a T.J. KIPPS

Vydání

Blood, WASHINGTON, American Society of Hematology, 2014, 0006-4971

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 10.452

Kód RIV

RIV/00216224:14740/14:00078422

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1182/blood-2013-09-527234

UT WoS

000342618300015

EID Scopus

2-s2.0-84903977667

Klíčová slova anglicky

GENE MUTATION STATUS; DOWN-REGULATION; C-MYB; DISEASE PROGRESSION; PROTEIN EXPRESSION; CD38 EXPRESSION; CLL PATIENTS; MICRORNAS; ZAP-70; APOPTOSIS

Štítky

kontrola MP, MP, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 6. 3. 2015 11:02, Martina Prášilová

Anotace

V originále

We examined the microRNAs (miRNAs) expressed in chronic lymphocytic leukemia (CLL) and identified miR-150 as the most abundant, but with leukemia cell expression levels that varied among patients. CLL cells that expressed zeta-chain-associated protein of 70 kDa (ZAP-70) or that used unmutated immunoglobulin heavy chain variable (IGHV) genes, each had a median expression level of miR-150 that was significantly lower than that of ZAP-70-negative CLL cells or those that used mutated IGHV genes. In samples stratified for expression of miR-150, CLL cells with low-level miR-150 expressed relatively higher levels of forkhead box P1 (FOXP1) and GRB2-associated binding protein 1 (GAB1), genes with 3' untranslated regions having evolutionary-conserved binding sites for miR-150. High-level expression of miR-150 could repress expression of these genes, which encode proteins that enhance B-cell receptor signaling, a putative CLL-growth/survival signal. Also, high-level expression of miR-150 was a significant independent predictor of longer treatment-free survival or overall survival, whereas an inverse association was observed for high-level expression of GAB1 or FOXP1 for overall survival. This study demonstrates that expression of miR-150 can influence the relative expression of GAB1 and FOXP1 and the signaling potential of the B-cell receptor, thereby possibly accounting for the noted association of expression of miR-150 and disease outcome.

Návaznosti

EE2.3.30.0009, projekt VaV

Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

Citovat

MRÁZ, Marek; L.G. CHEN; L.Z. RASSENTI; E.M. GHIA; H.Y. LI; K. JEPSEN; E.N. SMITH; K. MESSER; K.A. FRAZER a T.J. KIPPS. miR-150 influences B-cell receptor signaling in chronic lymphocytic leukemia by regulating expression of GAB1 and FOXP1. Blood. WASHINGTON: American Society of Hematology, 2014, roč. 124, č. 1, s. 84-95. ISSN 0006-4971. Dostupné z: https://doi.org/10.1182/blood-2013-09-527234.

@article{1216834,
   author = {Mráz, Marek and Chen, L.G. and Rassenti, L.Z. and Ghia, E.M. and Li, H.Y. and Jepsen, K. and Smith, E.N. and Messer, K. and Frazer, K.A. and Kipps, T.J.},
   article_location = {WASHINGTON},
   article_number = {1},
   doi = {https://doi.org/10.1182/blood-2013-09-527234},
   keywords = {GENE MUTATION STATUS; DOWN-REGULATION; C-MYB; DISEASE PROGRESSION; PROTEIN EXPRESSION; CD38 EXPRESSION; CLL PATIENTS; MICRORNAS; ZAP-70; APOPTOSIS},
   language = {eng},
   issn = {0006-4971},
   journal = {Blood},
   title = {miR-150 influences B-cell receptor signaling in chronic lymphocytic leukemia by regulating expression of GAB1 and FOXP1},
   url = {http://www.bloodjournal.org/content/124/1/84},
   volume = {124},
   year = {2014}
}

TY  - JOUR
ID  - 1216834
AU  - Mráz, Marek - Chen, L.G. - Rassenti, L.Z. - Ghia, E.M. - Li, H.Y. - Jepsen, K. - Smith, E.N. - Messer, K. - Frazer, K.A. - Kipps, T.J.
PY  - 2014
TI  - miR-150 influences B-cell receptor signaling in chronic lymphocytic leukemia by regulating expression of GAB1 and FOXP1
JF  - Blood
VL  - 124
IS  - 1
SP  - 84-95
EP  - 84-95
PB  - American Society of Hematology
SN  - 00064971
KW  - GENE MUTATION STATUS
KW  - DOWN-REGULATION
KW  - C-MYB
KW  - DISEASE PROGRESSION
KW  - PROTEIN EXPRESSION
KW  - CD38 EXPRESSION
KW  - CLL PATIENTS
KW  - MICRORNAS
KW  - ZAP-70
KW  - APOPTOSIS
UR  - http://www.bloodjournal.org/content/124/1/84
L2  - http://www.bloodjournal.org/content/124/1/84
N2  - We examined the microRNAs (miRNAs) expressed in chronic lymphocytic leukemia (CLL) and identified miR-150 as the most abundant, but with leukemia cell expression levels that varied among patients. CLL cells that expressed zeta-chain-associated protein of 70 kDa (ZAP-70) or that used unmutated immunoglobulin heavy chain variable (IGHV) genes, each had a median expression level of miR-150 that was significantly lower than that of ZAP-70-negative CLL cells or those that used mutated IGHV genes. In samples stratified for expression of miR-150, CLL cells with low-level miR-150 expressed relatively higher levels of forkhead box P1 (FOXP1) and GRB2-associated binding protein 1 (GAB1), genes with 3' untranslated regions having evolutionary-conserved binding sites for miR-150. High-level expression of miR-150 could repress expression of these genes, which encode proteins that enhance B-cell receptor signaling, a putative CLL-growth/survival signal. Also, high-level expression of miR-150 was a significant independent predictor of longer treatment-free survival or overall survival, whereas an inverse association was observed for high-level expression of GAB1 or FOXP1 for overall survival. This study demonstrates that expression of miR-150 can influence the relative expression of GAB1 and FOXP1 and the signaling potential of the B-cell receptor, thereby possibly accounting for the noted association of expression of miR-150 and disease outcome.
ER  -

MRÁZ, Marek; L.G. CHEN; L.Z. RASSENTI; E.M. GHIA; H.Y. LI; K. JEPSEN; E.N. SMITH; K. MESSER; K.A. FRAZER a T.J. KIPPS. miR-150 influences B-cell receptor signaling in chronic lymphocytic leukemia by regulating expression of GAB1 and FOXP1. \textit{Blood}. WASHINGTON: American Society of Hematology, 2014, roč.~124, č.~1, s.~84-95. ISSN~0006-4971. Dostupné z: https://doi.org/10.1182/blood-2013-09-527234.

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