KUDEROVÁ, Alena, Lucia GALLOVÁ, Katarína KURICOVÁ, Eliška NEJEDLÁ, Anna ČURDOVÁ, Lenka MICENKOVÁ, Ondřej PLÍHAL, David ŠMAJS, Lukáš SPÍCHAL and Jan HEJÁTKO. Identification of AHK2- and AHK3-like cytokinin receptors in Brassica napus reveals two subfamilies of AHK2 orthologues. Journal of Experimental Botany. Oxford: OXFORD UNIV PRESS, 2015, vol. 66, No 1, p. 339-353. ISSN 0022-0957. doi:10.1093/jxb/eru422.
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Basic information
Original name Identification of AHK2- and AHK3-like cytokinin receptors in Brassica napus reveals two subfamilies of AHK2 orthologues
Authors KUDEROVÁ, Alena (203 Czech Republic, belonging to the institution), Lucia GALLOVÁ (203 Czech Republic), Katarína KURICOVÁ (703 Slovakia, belonging to the institution), Eliška NEJEDLÁ (203 Czech Republic, belonging to the institution), Anna ČURDOVÁ (703 Slovakia, belonging to the institution), Lenka MICENKOVÁ (703 Slovakia, belonging to the institution), Ondřej PLÍHAL (203 Czech Republic), David ŠMAJS (203 Czech Republic, belonging to the institution), Lukáš SPÍCHAL (203 Czech Republic) and Jan HEJÁTKO (203 Czech Republic, guarantor, belonging to the institution).
Edition Journal of Experimental Botany, Oxford, OXFORD UNIV PRESS, 2015, 0022-0957.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 5.677
RIV identification code RIV/00216224:14740/15:00080639
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1093/jxb/eru422
UT WoS 000351660900028
Keywords in English CHASE-containing His kinase; E. coli-based live-cell competitive receptor assay; gene structure; JBnB library; modular protein architecture; phylogenetic analysis
Tags EL OK, podil, rivok
Tags International impact, Reviewed
Changed by Changed by: Martina Prášilová, učo 342282. Changed: 19. 8. 2015 10:44.
Abstract
Cytokinin (CK) signalling is known to play key roles in the regulation of plant growth and development, crop yields, and tolerance to both abiotic stress and pathogen defences, but the mechanisms involved are poorly characterized in dicotyledonous crops. Here the identification and functional characterization of sensor histidine kinases homologous to Arabidopsis CK receptors AHK2 and AHK3 in winter oilseed rape are presented. Five CHASE-containing His kinases were identified in Brassica napus var. Tapidor (BnCHK1–BnCHK5) by heterologous hybridization of its genomic library with gene-specific probes from Arabidopsis. The identified bacterial artificial chromosome (BAC) clones were fingerprinted and representative clones in five distinct groups were sequenced. Using a bioinformatic approach and cDNA cloning, the precise gene and putative protein domain structures were determined. Based on phylogenetic analysis, four AHK2 (BnCHK1–BnCHK4) homologues and one AHK3 (BnCHK5) homologue were defined. It is further suggested that BnCHK1 and BnCHK3, and BnCHK5 are orthologues of AHK2 and AHK3, originally from the B. rapa A genome, respectively. BnCHK1, BnCHK3, and BnCHK5 displayed high affinity for trans-zeatin (1–3nM) in a live-cell competitive receptor assay, but not with other plant hormones (indole acetic acid, GA3, and abscisic acid), confirming the prediction that they are genuine CK receptors. It is shown that BnCHK1 and BnCHK3, and BnCHK5 display distinct preferences for various CK bases and metabolites, characteristic of their AHK counterparts, AHK2 and AHK3, respectively. Interestingly, the AHK2 homologues could be divided into two subfamilies (BnCHK1/BnCK2 and BnCHK3/BnCHK4) that differ in putative transmembrane domain topology and CK binding specificity, thus implying potential functional divergence.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
EE2.3.20.0043, research and development projectName: Rozvoj lidských zdrojů pro výzkum, vývoj a inovace v oblasti genomiky a proteomiky rostlinných systémů
EE2.3.20.0189, research and development projectName: Rozvoj výzkumné excelence v oblasti evoluční cytogenomiky, epigenetiky a buněčné signalizace
GAP305/11/0756, research and development projectName: Strukturní princip specificity přenosu signálu v rostlinách: síť interakcí příjímačové domény histidinkinas u Arabidospis (Acronym: HKRD-AHP)
Investor: Czech Science Foundation
GA13-25280S, research and development projectName: Elucidating molecular mechanisms of cytokinins-ethylene crosstalk in the plant development
Investor: Czech Science Foundation
GA13-39982S, research and development projectName: Molekulární mechanismy auxín-cytokinínové interakce v regulaci rostlinné organogenese.
Investor: Czech Science Foundation
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