Identification of AHK2- and AHK3-like cytokinin receptors in
Brassica napus reveals two subfamilies of AHK2 orthologues

J 2015

Identification of AHK2- and AHK3-like cytokinin receptors in Brassica napus reveals two subfamilies of AHK2 orthologues

KUDEROVÁ, Alena, Lucia GALLOVÁ, Katarína KURICOVÁ, Eliška NEJEDLÁ, Anna ČURDOVÁ et. al.

Basic information

Original name

Identification of AHK2- and AHK3-like cytokinin receptors in Brassica napus reveals two subfamilies of AHK2 orthologues

Authors

KUDEROVÁ, Alena (203 Czech Republic, belonging to the institution), Lucia GALLOVÁ (203 Czech Republic), Katarína KURICOVÁ (703 Slovakia, belonging to the institution), Eliška NEJEDLÁ (203 Czech Republic, belonging to the institution), Anna ČURDOVÁ (703 Slovakia, belonging to the institution), Lenka MICENKOVÁ (703 Slovakia, belonging to the institution), Ondřej PLÍHAL (203 Czech Republic), David ŠMAJS (203 Czech Republic, belonging to the institution), Lukáš SPÍCHAL (203 Czech Republic) and Jan HEJÁTKO (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Experimental Botany, Oxford, OXFORD UNIV PRESS, 2015, 0022-0957

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.677

RIV identification code

RIV/00216224:14740/15:00080639

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1093/jxb/eru422

UT WoS

000351660900028

Keywords in English

CHASE-containing His kinase; E. coli-based live-cell competitive receptor assay; gene structure; JBnB library; modular protein architecture; phylogenetic analysis

Abstract

V originále

Cytokinin (CK) signalling is known to play key roles in the regulation of plant growth and development, crop yields, and tolerance to both abiotic stress and pathogen defences, but the mechanisms involved are poorly characterized in dicotyledonous crops. Here the identification and functional characterization of sensor histidine kinases homologous to Arabidopsis CK receptors AHK2 and AHK3 in winter oilseed rape are presented. Five CHASE-containing His kinases were identified in Brassica napus var. Tapidor (BnCHK1–BnCHK5) by heterologous hybridization of its genomic library with gene-specific probes from Arabidopsis. The identified bacterial artificial chromosome (BAC) clones were fingerprinted and representative clones in five distinct groups were sequenced. Using a bioinformatic approach and cDNA cloning, the precise gene and putative protein domain structures were determined. Based on phylogenetic analysis, four AHK2 (BnCHK1–BnCHK4) homologues and one AHK3 (BnCHK5) homologue were defined. It is further suggested that BnCHK1 and BnCHK3, and BnCHK5 are orthologues of AHK2 and AHK3, originally from the B. rapa A genome, respectively. BnCHK1, BnCHK3, and BnCHK5 displayed high affinity for trans-zeatin (1–3nM) in a live-cell competitive receptor assay, but not with other plant hormones (indole acetic acid, GA3, and abscisic acid), confirming the prediction that they are genuine CK receptors. It is shown that BnCHK1 and BnCHK3, and BnCHK5 display distinct preferences for various CK bases and metabolites, characteristic of their AHK counterparts, AHK2 and AHK3, respectively. Interestingly, the AHK2 homologues could be divided into two subfamilies (BnCHK1/BnCK2 and BnCHK3/BnCHK4) that differ in putative transmembrane domain topology and CK binding specificity, thus implying potential functional divergence.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

EE2.3.20.0043, research and development project

Name: Rozvoj lidských zdrojů pro výzkum, vývoj a inovace v oblasti genomiky a proteomiky rostlinných systémů

EE2.3.20.0189, research and development project

Name: Rozvoj výzkumné excelence v oblasti evoluční cytogenomiky, epigenetiky a buněčné signalizace

GAP305/11/0756, research and development project

Name: Strukturní princip specificity přenosu signálu v rostlinách: síť interakcí příjímačové domény histidinkinas u Arabidospis (Acronym: HKRD-AHP)

Investor: Czech Science Foundation

GA13-25280S, research and development project

Name: Elucidating molecular mechanisms of cytokinins-ethylene crosstalk in the plant development

Investor: Czech Science Foundation

GA13-39982S, research and development project

Name: Molekulární mechanismy auxín-cytokinínové interakce v regulaci rostlinné organogenese.

Investor: Czech Science Foundation

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Citovat

KUDEROVÁ, Alena, Lucia GALLOVÁ, Katarína KURICOVÁ, Eliška NEJEDLÁ, Anna ČURDOVÁ, Lenka MICENKOVÁ, Ondřej PLÍHAL, David ŠMAJS, Lukáš SPÍCHAL and Jan HEJÁTKO. Identification of AHK2- and AHK3-like cytokinin receptors in Brassica napus reveals two subfamilies of AHK2 orthologues. Journal of Experimental Botany. Oxford: OXFORD UNIV PRESS, 2015, vol. 66, No 1, p. 339-353. ISSN 0022-0957. Available from: https://dx.doi.org/10.1093/jxb/eru422.

@article{1217495,
   author = {Kuderová, Alena and Gallová, Lucia and Kuricová, Katarína and Nejedlá, Eliška and Čurdová, Anna and Micenková, Lenka and Plíhal, Ondřej and Šmajs, David and Spíchal, Lukáš and Hejátko, Jan},
   article_location = {Oxford},
   article_number = {1},
   doi = {http://dx.doi.org/10.1093/jxb/eru422},
   keywords = {CHASE-containing His kinase; E. coli-based live-cell competitive receptor assay; gene structure; JBnB library; modular protein architecture; phylogenetic analysis},
   language = {eng},
   issn = {0022-0957},
   journal = {Journal of Experimental Botany},
   title = {Identification of AHK2- and AHK3-like cytokinin receptors in Brassica napus reveals two subfamilies of AHK2 orthologues},
   url = {http://jxb.oxfordjournals.org/content/early/2014/10/20/jxb.eru422.full.pdf},
   volume = {66},
   year = {2015}
}

TY  - JOUR
ID  - 1217495
AU  - Kuderová, Alena - Gallová, Lucia - Kuricová, Katarína - Nejedlá, Eliška - Čurdová, Anna - Micenková, Lenka - Plíhal, Ondřej - Šmajs, David - Spíchal, Lukáš - Hejátko, Jan
PY  - 2015
TI  - Identification of AHK2- and AHK3-like cytokinin receptors in Brassica napus reveals two subfamilies of AHK2 orthologues
JF  - Journal of Experimental Botany
VL  - 66
IS  - 1
SP  - 339-353
EP  - 339-353
PB  - OXFORD UNIV PRESS
SN  - 00220957
KW  - CHASE-containing His kinase
KW  - E. coli-based live-cell competitive receptor assay
KW  - gene structure
KW  - JBnB library
KW  - modular protein architecture
KW  - phylogenetic analysis
UR  - http://jxb.oxfordjournals.org/content/early/2014/10/20/jxb.eru422.full.pdf
L2  - http://jxb.oxfordjournals.org/content/early/2014/10/20/jxb.eru422.full.pdf
N2  - Cytokinin (CK) signalling is known to play key roles in the regulation of plant growth and development, crop yields, and tolerance to both abiotic stress and pathogen defences, but the mechanisms involved are poorly characterized in dicotyledonous crops. Here the identification and functional characterization of sensor histidine kinases homologous to Arabidopsis CK receptors AHK2 and AHK3 in winter oilseed rape are presented. Five CHASE-containing His kinases were identified in Brassica napus var. Tapidor (BnCHK1–BnCHK5) by heterologous hybridization of its genomic library with gene-specific probes from Arabidopsis. The identified bacterial artificial chromosome (BAC) clones were fingerprinted and representative clones in five distinct groups were sequenced. Using a bioinformatic approach and cDNA cloning, the precise gene and putative protein domain structures were determined. Based on phylogenetic analysis, four AHK2 (BnCHK1–BnCHK4) homologues and one AHK3 (BnCHK5) homologue were defined. It is further suggested that BnCHK1 and BnCHK3, and BnCHK5 are orthologues of AHK2 and AHK3, originally from the B. rapa A genome, respectively. BnCHK1, BnCHK3, and BnCHK5 displayed high affinity for trans-zeatin (1–3nM) in a live-cell competitive receptor assay, but not with other plant hormones (indole acetic acid, GA3, and abscisic acid), confirming the prediction that they are genuine CK receptors. It is shown that BnCHK1 and BnCHK3, and BnCHK5 display distinct preferences for various CK bases and metabolites, characteristic of their AHK counterparts, AHK2 and AHK3, respectively. Interestingly, the AHK2 homologues could be divided into two subfamilies (BnCHK1/BnCK2 and BnCHK3/BnCHK4) that differ in putative transmembrane domain topology and CK binding specificity, thus implying potential functional divergence.
ER  -

KUDEROVÁ, Alena, Lucia GALLOVÁ, Katarína KURICOVÁ, Eliška NEJEDLÁ, Anna ČURDOVÁ, Lenka MICENKOVÁ, Ondřej PLÍHAL, David ŠMAJS, Lukáš SPÍCHAL and Jan HEJÁTKO. Identification of AHK2- and AHK3-like cytokinin receptors in Brassica napus reveals two subfamilies of AHK2 orthologues. \textit{Journal of Experimental Botany}. Oxford: OXFORD UNIV PRESS, 2015, vol.~66, No~1, p.~339-353. ISSN~0022-0957. Available from: https://dx.doi.org/10.1093/jxb/eru422.

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