Identification of quantum dots labeled metallothionein by fast
scanning laser-induced breakdown spectroscopy

J 2014

Identification of quantum dots labeled metallothionein by fast scanning laser-induced breakdown spectroscopy

KONEČNÁ, Marie, Karel NOVOTNÝ, Soňa KŘÍŽKOVÁ, Iva BLAŽKOVÁ, Pavel KOPEL et. al.

Basic information

Original name

Identification of quantum dots labeled metallothionein by fast scanning laser-induced breakdown spectroscopy

Authors

KONEČNÁ, Marie (203 Czech Republic), Karel NOVOTNÝ (203 Czech Republic, guarantor, belonging to the institution), Soňa KŘÍŽKOVÁ (203 Czech Republic), Iva BLAŽKOVÁ (203 Czech Republic), Pavel KOPEL (203 Czech Republic), Jozef KAISER (203 Czech Republic), Petr HODEK (203 Czech Republic), René KIZEK (203 Czech Republic) and Vojtěch ADAM (203 Czech Republic)

Edition

Spectrochimica Acta B, Elsevier, 2014, 0584-8547

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.176

RIV identification code

RIV/00216224:14740/14:00078587

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.sab.2014.08.037

UT WoS

000344440300031

Keywords in English

CANCER; NANOPARTICLES; IMMUNOASSAYS; ANTIBODIES; CHEMISTRY

Abstract

V originále

The technique described in this paper allows detection of quantum dots (QDs) specifically deposited on the polystyrene surface by laser-induced breakdown spectroscopy (LIBS). Using LIBS, the distribution of QDs or their conjugates with biomolecules deposited on the surface can be observed, regardless of the fact if they exhibit fluorescence or not. QDs deposited on the specific surface of polystyrene microplate in the form of spots are detected by determination of the metal included in the QDs structure. Cd-containing QDs (CdS, CdTe) stabilized with mercaptopropionic (MPA) or mercaptosuccinic (MSA) acid, respectively, alone or in the form of conjugates with metallothionein (MT) biomolecule are determined by using the 508.58 nm Cd emission line. The observed absolute detection limit for Cd in CdTe QDs conjugates with MT in one spot was 3 ng Cd. Due to the high sensitivity of this technique, the immunoanalysis in combination with LIBS was also investigated. Cd spatial distribution in sandwich immunoassay was detected.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

Citovat

KONEČNÁ, Marie, Karel NOVOTNÝ, Soňa KŘÍŽKOVÁ, Iva BLAŽKOVÁ, Pavel KOPEL, Jozef KAISER, Petr HODEK, René KIZEK and Vojtěch ADAM. Identification of quantum dots labeled metallothionein by fast scanning laser-induced breakdown spectroscopy. Spectrochimica Acta B. Elsevier, 2014, vol. 101, November, p. 220-225. ISSN 0584-8547. Available from: https://dx.doi.org/10.1016/j.sab.2014.08.037.

@article{1217792,
   author = {Konečná, Marie and Novotný, Karel and Křížková, Soňa and Blažková, Iva and Kopel, Pavel and Kaiser, Jozef and Hodek, Petr and Kizek, René and Adam, Vojtěch},
   article_number = {November},
   doi = {http://dx.doi.org/10.1016/j.sab.2014.08.037},
   keywords = {CANCER; NANOPARTICLES; IMMUNOASSAYS; ANTIBODIES; CHEMISTRY},
   language = {eng},
   issn = {0584-8547},
   journal = {Spectrochimica Acta B},
   title = {Identification of quantum dots labeled metallothionein by fast scanning laser-induced breakdown spectroscopy},
   url = {http://www.sciencedirect.com/science/article/pii/S0584854714002134},
   volume = {101},
   year = {2014}
}

TY  - JOUR
ID  - 1217792
AU  - Konečná, Marie - Novotný, Karel - Křížková, Soňa - Blažková, Iva - Kopel, Pavel - Kaiser, Jozef - Hodek, Petr - Kizek, René - Adam, Vojtěch
PY  - 2014
TI  - Identification of quantum dots labeled metallothionein by fast scanning laser-induced breakdown spectroscopy
JF  - Spectrochimica Acta B
VL  - 101
IS  - November
SP  - 220-225
EP  - 220-225
PB  - Elsevier
SN  - 05848547
KW  - CANCER
KW  - NANOPARTICLES
KW  - IMMUNOASSAYS
KW  - ANTIBODIES
KW  - CHEMISTRY
UR  - http://www.sciencedirect.com/science/article/pii/S0584854714002134
L2  - http://www.sciencedirect.com/science/article/pii/S0584854714002134
N2  - The technique described in this paper allows detection of quantum dots (QDs) specifically deposited on the polystyrene surface by laser-induced breakdown spectroscopy (LIBS). Using LIBS, the distribution of QDs or their conjugates with biomolecules deposited on the surface can be observed, regardless of the fact if they exhibit fluorescence or not. QDs deposited on the specific surface of polystyrene microplate in the form of spots are detected by determination of the metal included in the QDs structure. Cd-containing QDs (CdS, CdTe) stabilized with mercaptopropionic (MPA) or mercaptosuccinic (MSA) acid, respectively, alone or in the form of conjugates with metallothionein (MT) biomolecule are determined by using the 508.58 nm Cd emission line. The observed absolute detection limit for Cd in CdTe QDs conjugates with MT in one spot was 3 ng Cd. Due to the high sensitivity of this technique, the immunoanalysis in combination with LIBS was also investigated. Cd spatial distribution in sandwich immunoassay was detected.
ER  -

KONEČNÁ, Marie, Karel NOVOTNÝ, Soňa KŘÍŽKOVÁ, Iva BLAŽKOVÁ, Pavel KOPEL, Jozef KAISER, Petr HODEK, René KIZEK and Vojtěch ADAM. Identification of quantum dots labeled metallothionein by fast scanning laser-induced breakdown spectroscopy. \textit{Spectrochimica Acta B}. Elsevier, 2014, vol.~101, November, p.~220-225. ISSN~0584-8547. Available from: https://dx.doi.org/10.1016/j.sab.2014.08.037.

Detailed Information on Publication Record