KONEČNÁ, Marie, Karel NOVOTNÝ, Soňa KŘÍŽKOVÁ, Iva BLAŽKOVÁ, Pavel KOPEL, Jozef KAISER, Petr HODEK, René KIZEK and Vojtěch ADAM. Identification of quantum dots labeled metallothionein by fast scanning laser-induced breakdown spectroscopy. Spectrochimica Acta B. Elsevier, 2014, vol. 101, November, p. 220-225. ISSN 0584-8547. Available from: https://dx.doi.org/10.1016/j.sab.2014.08.037. |
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@article{1217792, author = {Konečná, Marie and Novotný, Karel and Křížková, Soňa and Blažková, Iva and Kopel, Pavel and Kaiser, Jozef and Hodek, Petr and Kizek, René and Adam, Vojtěch}, article_number = {November}, doi = {http://dx.doi.org/10.1016/j.sab.2014.08.037}, keywords = {CANCER; NANOPARTICLES; IMMUNOASSAYS; ANTIBODIES; CHEMISTRY}, language = {eng}, issn = {0584-8547}, journal = {Spectrochimica Acta B}, title = {Identification of quantum dots labeled metallothionein by fast scanning laser-induced breakdown spectroscopy}, url = {http://www.sciencedirect.com/science/article/pii/S0584854714002134}, volume = {101}, year = {2014} }
TY - JOUR ID - 1217792 AU - Konečná, Marie - Novotný, Karel - Křížková, Soňa - Blažková, Iva - Kopel, Pavel - Kaiser, Jozef - Hodek, Petr - Kizek, René - Adam, Vojtěch PY - 2014 TI - Identification of quantum dots labeled metallothionein by fast scanning laser-induced breakdown spectroscopy JF - Spectrochimica Acta B VL - 101 IS - November SP - 220-225 EP - 220-225 PB - Elsevier SN - 05848547 KW - CANCER KW - NANOPARTICLES KW - IMMUNOASSAYS KW - ANTIBODIES KW - CHEMISTRY UR - http://www.sciencedirect.com/science/article/pii/S0584854714002134 L2 - http://www.sciencedirect.com/science/article/pii/S0584854714002134 N2 - The technique described in this paper allows detection of quantum dots (QDs) specifically deposited on the polystyrene surface by laser-induced breakdown spectroscopy (LIBS). Using LIBS, the distribution of QDs or their conjugates with biomolecules deposited on the surface can be observed, regardless of the fact if they exhibit fluorescence or not. QDs deposited on the specific surface of polystyrene microplate in the form of spots are detected by determination of the metal included in the QDs structure. Cd-containing QDs (CdS, CdTe) stabilized with mercaptopropionic (MPA) or mercaptosuccinic (MSA) acid, respectively, alone or in the form of conjugates with metallothionein (MT) biomolecule are determined by using the 508.58 nm Cd emission line. The observed absolute detection limit for Cd in CdTe QDs conjugates with MT in one spot was 3 ng Cd. Due to the high sensitivity of this technique, the immunoanalysis in combination with LIBS was also investigated. Cd spatial distribution in sandwich immunoassay was detected. ER -
KONEČNÁ, Marie, Karel NOVOTNÝ, Soňa KŘÍŽKOVÁ, Iva BLAŽKOVÁ, Pavel KOPEL, Jozef KAISER, Petr HODEK, René KIZEK and Vojtěch ADAM. Identification of quantum dots labeled metallothionein by fast scanning laser-induced breakdown spectroscopy. \textit{Spectrochimica Acta B}. Elsevier, 2014, vol.~101, November, p.~220-225. ISSN~0584-8547. Available from: https://dx.doi.org/10.1016/j.sab.2014.08.037.
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