Identification of quantum dots labeled metallothionein by fast scanning laser-induced breakdown spectroscopy

KONEČNÁ, Marie, Karel NOVOTNÝ, Soňa KŘÍŽKOVÁ, Iva BLAŽKOVÁ, Pavel KOPEL, Jozef KAISER, Petr HODEK, René KIZEK and Vojtěch ADAM. Identification of quantum dots labeled metallothionein by fast scanning laser-induced breakdown spectroscopy. Spectrochimica Acta B. Elsevier, vol. 101, November, p. 220-225. ISSN 0584-8547. doi:10.1016/j.sab.2014.08.037. 2014.

Basic information

• Original name: Identification of quantum dots labeled metallothionein by fast scanning laser-induced breakdown spectroscopy
• Authors: KONEČNÁ, Marie (203 Czech Republic), Karel NOVOTNÝ (203 Czech Republic, guarantor, belonging to the institution), Soňa KŘÍŽKOVÁ (203 Czech Republic), Iva BLAŽKOVÁ (203 Czech Republic), Pavel KOPEL (203 Czech Republic), Jozef KAISER (203 Czech Republic), Petr HODEK (203 Czech Republic), René KIZEK (203 Czech Republic) and Vojtěch ADAM (203 Czech Republic).
• Edition: Spectrochimica Acta B, Elsevier, 2014, 0584-8547.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10406 Analytical chemistry
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 3.176
• RIV identification code: RIV/00216224:14740/14:00078587
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1016/j.sab.2014.08.037
• UT WoS: 000344440300031
• Keywords in English: CANCER; NANOPARTICLES; IMMUNOASSAYS; ANTIBODIES; CHEMISTRY
• Tags: kontrola MP, podil, rivok
• Tags: International impact, Reviewed

Abstract

The technique described in this paper allows detection of quantum dots (QDs) specifically deposited on the polystyrene surface by laser-induced breakdown spectroscopy (LIBS). Using LIBS, the distribution of QDs or their conjugates with biomolecules deposited on the surface can be observed, regardless of the fact if they exhibit fluorescence or not. QDs deposited on the specific surface of polystyrene microplate in the form of spots are detected by determination of the metal included in the QDs structure. Cd-containing QDs (CdS, CdTe) stabilized with mercaptopropionic (MPA) or mercaptosuccinic (MSA) acid, respectively, alone or in the form of conjugates with metallothionein (MT) biomolecule are determined by using the 508.58 nm Cd emission line. The observed absolute detection limit for Cd in CdTe QDs conjugates with MT in one spot was 3 ng Cd. Due to the high sensitivity of this technique, the immunoanalysis in combination with LIBS was also investigated. Cd spatial distribution in sandwich immunoassay was detected.

Links

• ED1.1.00/02.0068, research and development project: Name: CEITEC - central european institute of technology