J 2014

Escherichia coli strains of phylogenetic group B2 and D and bacteriocin production are associated with advanced colorectal neoplasia

KOHOUTOVA, Darina, David ŠMAJS, Paula MORAVKOVA, Jiri CYRANY, Monika MORAVKOVA et. al.

Basic information

Original name

Escherichia coli strains of phylogenetic group B2 and D and bacteriocin production are associated with advanced colorectal neoplasia

Authors

KOHOUTOVA, Darina (203 Czech Republic), David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution), Paula MORAVKOVA (203 Czech Republic), Jiri CYRANY (203 Czech Republic), Monika MORAVKOVA (203 Czech Republic), Miroslava FORSTLOVA (203 Czech Republic), Michal CIHAK (203 Czech Republic), Stanislav REJCHRT (203 Czech Republic) and Jan BURES (203 Czech Republic)

Edition

BMC Infectious Diseases, London, BioMed Central, 2014, 1471-2334

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.613

RIV identification code

RIV/00216224:14110/14:00078631

Organization unit

Faculty of Medicine

DOI

UT WoS

000209711400001

Keywords in English

Escherichia coli; Colicins; Bacteriocins; Colorectal adenoma; Colorectal carcinoma

Tags

Tags

International impact, Reviewed
Změněno: 23/1/2015 14:19, Soňa Böhmová

Abstract

V originále

Background: Colorectal cancer (CRC) is the 3rd most common cancer worldwide and the Czech Republic has the 6th highest incidence of CRC worldwide. Large intestinal microbiota play in its etiopathogenesis important role. Bacteriocins are proteins, produced by bacteria from the Enterobacteriaceae family. The aim of our prospective study was to assess the colonization of large intestinal mucosa by Escherichia coli strains and to investigate their bacteriocin production. Methods: A total of 30 consecutive patients with colorectal adenoma, CRA (17 men, 13 women, aged 39–79, mean age 63 +/- 9), 30 patients with CRC (23 men, 7 women, aged 38–86, mean age 67 +/- 11) and 20 healthy controls (9 men, 11 women, age 23–84, mean age 55 +/- 15) were enrolled into prospective study. Mucosal biopsies were taken in the caecum, transverse colon and rectum during pancolonoscopy. Microbiological culture, isolation and identification of bacteria followed. Bacteriocin production was assessed by growth inhibition of indicator strains E. coli K12-Row, E. coli C6 (phi), and Shigella sonnei 17. Identification of bacteriocin-encoding determinants and E. coli phylogroups was performed using PCR methods. Results: A total of 622 strains were isolated and further investigated. A significantly higher frequency of simultaneous production of colicins and microcins was revealed in the group of patients with CRC, when compared to patients with CRA, p = 0.031. A significantly higher frequency of E. coli phylogroup D was found in patients with CRC, when compared to controls, p = 0.044. A significantly higher prevalence of bacteriocinogeny was confirmed in patients with advanced adenoma when compared to patients with non-advanced adenoma, p = 0.010. Increasing bacteriocinogeny was associated with an increasing stage of CRC (assessed according to TNM classification). Either E. coli phylogroup B2 or E. coli phylogroup D were isolated in biopsies of patients with right-sided CRC. A statistically higher incidence of E. coli phylogroup B2 was found in patients with right-sided CRC when compared to patients with left-sided CRC, p=0.028. Conclusions: Large intestinal mucosa of patients with more advanced colorectal neoplasia is colonized with more virulent strains of E. coli and higher production of bacteriocins is observed in these patients when compared to those with less advanced colorectal neoplasia.