| HORAKOVA, Dana, Petra CELÁ, Pavel KREJČÍ, Lukáš BÁLEK, Simona MORAVCOVÁ BALKOVÁ, Eva MATALOVA a Marcela BUCHTOVA. Effect of FGFR inhibitors on chicken limb development. Development Growth & Differentiation. Hoboken, USA: WILEY-BLACKWELL, roč. 56, č. 8, s. 555-572. ISSN 0012-1592. doi:10.1111/dgd.12156. 2014. |
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@article{1218005,
author = {Horakova, Dana and Celá, Petra and Krejčí, Pavel and Bálek, Lukáš and Moravcová Balková, Simona and Matalova, Eva and Buchtova, Marcela},
article_location = {Hoboken, USA},
article_number = {8},
doi = {http://dx.doi.org/10.1111/dgd.12156},
keywords = {chondrogenesis; fibroblast growth factor receptor; mesenchymal condensation; zeugopod},
language = {eng},
issn = {0012-1592},
journal = {Development Growth & Differentiation},
title = {Effect of FGFR inhibitors on chicken limb development},
volume = {56},
year = {2014}
}
TY - JOUR
ID - 1218005
AU - Horakova, Dana - Celá, Petra - Krejčí, Pavel - Bálek, Lukáš - Moravcová Balková, Simona - Matalova, Eva - Buchtova, Marcela
PY - 2014
TI - Effect of FGFR inhibitors on chicken limb development
JF - Development Growth & Differentiation
VL - 56
IS - 8
SP - 555-572
EP - 555-572
PB - WILEY-BLACKWELL
SN - 00121592
KW - chondrogenesis
KW - fibroblast growth factor receptor
KW - mesenchymal condensation
KW - zeugopod
N2 - Fibroblast growth factor (FGF) signalling appears essential for the regulation of limb development, but a full complexity of this regulation remains unclear. Here, we addressed the effect of three different chemical inhibitors of FGF receptor tyrosine kinases (FGFR) on growth and patterning of the chicken wings. The inhibitor PD173074 caused shorter and thinner wing when using lower concentration. Microinjection of higher PD173074 concentrations (25 and 50mmol/L) into the wing bud at stage 20 resulted in the development of small wing rudiment or the total absence of the wing. Skeletal analysis revealed the absence of the radius but not ulna, deformation of metacarpal bones and/or a reduction of digits. Treatment with PD161570 resembled the effects of PD173074. NF449 induced shortening and deformation of the developing wing with reduced autopodium. These malformed embryos mostly died at the stage HH25-29. PD173074 reduced chondrogenesis also in the limb micromass cultures together with early inhibition of cartilaginous nodule formation, evidenced by lack of sulphated proteoglycan and peanut agglutinin expression. The effect of FGFR inhibition on limb development observed here was unlikely mediated by excessive cell death as none of the inhibitors caused massive apoptosis at low concentrations. More probably, FGFR inhibition decreased both the proliferation and adhesion of mesenchymal chondroprogenitors. We conclude that FGFR signalling contributes to the regulation of the anterior-posterior patterning of zeugopod during chicken limb development.
ER -
HORAKOVA, Dana, Petra CELÁ, Pavel KREJČÍ, Lukáš BÁLEK, Simona MORAVCOVÁ BALKOVÁ, Eva MATALOVA a Marcela BUCHTOVA. Effect of FGFR inhibitors on chicken limb development. \textit{Development Growth \&{} Differentiation}. Hoboken, USA: WILEY-BLACKWELL, roč.~56, č.~8, s.~555-572. ISSN~0012-1592. doi:10.1111/dgd.12156. 2014.
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