OCCURRENCE OF TOXOPLASMA GONDII IN DOMESTICATED PIGS AND WILD
BOARS BRED IN THE CZECH REPUBLIC

a 2015

OCCURRENCE OF TOXOPLASMA GONDII IN DOMESTICATED PIGS AND WILD BOARS BRED IN THE CZECH REPUBLIC

SLANÝ, Michal, Lea JAKUBCOVÁ, Nikol RESLOVÁ and Alena LORENCOVÁ

Basic information

Original name

OCCURRENCE OF TOXOPLASMA GONDII IN DOMESTICATED PIGS AND WILD BOARS BRED IN THE CZECH REPUBLIC

Authors

SLANÝ, Michal (203 Czech Republic, guarantor), Lea JAKUBCOVÁ (203 Czech Republic), Nikol RESLOVÁ (203 Czech Republic, belonging to the institution) and Alena LORENCOVÁ (203 Czech Republic)

Edition

First International Symposium on Veterinary Medicine 2015 (ISVM 2015), 2015

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/15:00082335

Organization unit

Faculty of Science

Keywords (in Czech)

real-time PCR; bezpečnost potravin; alimentární patogeny

Keywords in English

real-time PCR; food safety; food borne pathogen

Změněno: 13/1/2016 13:36, Mgr. Nikol Reslová, Ph.D.

Abstract

V originále

In recent years, there has been an increase in the numbers of reported outbreaks, cases, associated with food borne parasitic infections arising from meat consumption. Toxoplasma gondii is the fourth important agent according to the global ranking of foodborne parasites by “importance” and their primary food vehicle (WHO 2013). The aim of this study was to determine the occurrence of T. gondii in sources of pork meat represented by pigs and wild boars slaughtered in the Czech Republic. Muscle samples (diaphragma) collected from a total of 499 animals were collected during years 2013 and 2014. Domesticated pigs came from one organic (n=48) and four conventional farms (n=61). Wild boars originated from three game reserves (n=356) and two free hunt localities (n=34) in the Czech Republic. The detection of T. gondii was achieved by homemade qPCR assay targeting simultaneously locus 529 rep and B1. The higher incidence of T. gondii was observed in organic farm (29.2 %) when compared to. conventional farms (14.8 %). It is in accordance with published data suggesting that intensive production systems have eliminated Toxoplasma infection in pigs. However, organic farming where pigs are not separated from the outside environment may lead to reemergence of this pathogen. The presence of T. gondii was also confirmed in wild boars, but with no significant difference between game reseves (9.8 %) and free hunting areas (8.8 %). To conclude, pigs grown under organic farming are exposed to contaminated environment (frequent occurrence of cats, infected mice or rats) more significantly than conventionally farmed pigs or wild boars. The results show that pork meat poses a certain health risk and can be a potential source of foodborne disease. This risk is increased in cases of consumption of raw or undercooked meat.

Links

MUNI/A/1484/2014, interní kód MU

Name: Analýzy diverzity biologických systémů různých úrovní a na různých škálách terestrického a akvatického prostředí (Acronym: BIDA4)

Investor: Masaryk University, Category A

Citovat

SLANÝ, Michal, Lea JAKUBCOVÁ, Nikol RESLOVÁ and Alena LORENCOVÁ. OCCURRENCE OF TOXOPLASMA GONDII IN DOMESTICATED PIGS AND WILD BOARS BRED IN THE CZECH REPUBLIC. In First International Symposium on Veterinary Medicine 2015 (ISVM 2015). 2015.

@proceedings{1220043,
   author = {Slaný, Michal and Jakubcová, Lea and Reslová, Nikol and Lorencová, Alena},
   booktitle = {First International Symposium on Veterinary Medicine 2015 (ISVM 2015)},
   keywords = {real-time PCR; food safety; food borne pathogen},
   language = {eng},
   title = {OCCURRENCE OF TOXOPLASMA GONDII IN DOMESTICATED PIGS AND WILD BOARS BRED IN THE CZECH REPUBLIC},
   year = {2015}
}

TY  - CONF
ID  - 1220043
AU  - Slaný, Michal - Jakubcová, Lea - Reslová, Nikol - Lorencová, Alena
PY  - 2015
TI  - OCCURRENCE OF TOXOPLASMA GONDII IN DOMESTICATED PIGS AND WILD BOARS BRED IN THE CZECH REPUBLIC
KW  - real-time PCR
KW  - food safety
KW  - food borne pathogen
N2  - In recent years, there has been an increase in the numbers of reported outbreaks, cases, associated with food borne parasitic infections arising from meat consumption. Toxoplasma gondii is the fourth important agent according to the global ranking of foodborne parasites by “importance” and their primary food vehicle (WHO 2013). The aim of this study was to determine the occurrence of T. gondii in sources of pork meat represented by pigs and wild boars slaughtered in the Czech Republic. Muscle samples (diaphragma) collected from a total of 499 animals were collected during years 2013 and 2014. Domesticated pigs came from one organic (n=48) and four conventional farms (n=61). Wild boars originated from three game reserves (n=356) and two free hunt localities (n=34) in the Czech Republic. The detection of T. gondii was achieved by homemade qPCR assay targeting simultaneously locus 529 rep and B1. The higher incidence of T. gondii was observed in organic farm (29.2 %) when compared to. conventional farms (14.8 %). It is in accordance with published data suggesting that intensive production systems have eliminated Toxoplasma infection in pigs. However, organic farming where pigs are not separated from the outside environment may lead to reemergence of this pathogen. The presence of T. gondii was also confirmed in wild boars, but with no significant difference between game reseves (9.8 %) and free hunting areas (8.8 %). To conclude, pigs grown under organic farming are exposed to contaminated environment (frequent occurrence of cats, infected mice or rats) more significantly than conventionally farmed pigs or wild boars. The results show that pork meat poses a certain health risk and can be a potential source of foodborne disease. This risk is increased in cases of consumption of raw or undercooked meat.
ER  -

SLANÝ, Michal, Lea JAKUBCOVÁ, Nikol RESLOVÁ and Alena LORENCOVÁ. OCCURRENCE OF TOXOPLASMA GONDII IN DOMESTICATED PIGS AND WILD BOARS BRED IN THE CZECH REPUBLIC. In \textit{First International Symposium on Veterinary Medicine 2015 (ISVM 2015)}. 2015.

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