J 2014

Inflammatory Profiling of Schwann Cells in Contact with Growing Axons Distal to Nerve Injury

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ and Ivana HRADILOVÁ SVÍŽENSKÁ

Basic information

Original name

Inflammatory Profiling of Schwann Cells in Contact with Growing Axons Distal to Nerve Injury

Authors

DUBOVÝ, Petr (203 Czech Republic, guarantor, belonging to the institution), Ilona KLUSÁKOVÁ (203 Czech Republic, belonging to the institution) and Ivana HRADILOVÁ SVÍŽENSKÁ (203 Czech Republic, belonging to the institution)

Edition

BioMed Research International, USA, Hindawi Publishing Corporation, 2014, 2314-6133

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30000 3. Medical and Health Sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 1.579

RIV identification code

RIV/00216224:14740/14:00078951

Organization unit

Central European Institute of Technology

UT WoS

000335836400001

Keywords in English

activated Schwann cell; growing axons; reinnervation; inflammatory mediators

Tags

International impact, Reviewed
Změněno: 17/4/2015 09:09, Martina Prášilová

Abstract

V originále

Activated Schwann cells distal to nerve injury upregulate inflammatory mediators, including cytokines. The goal of the present study was to investigate expression of proinflammatory (IL-1b, TNFa) and anti-inflammatory cytokines (IL-4, IL-10) in activated Schwann cells in relation to growing axons distal to crush injury of rat sciatic nerves. Seven days fromsciatic nerve crush, transverse cryostat sections were cut 5 mm distal to lesion and incubated for double immunostaining to indicate Schwann cells (GFAP or S100b) and individual investigated cytokines or to demonstrate growing axons (GAP43). The Schwann cells of naive sciatic nerves and those removed from sham-operated rats displayed similar weak immunoreactivity for the investigated cytokines. In contrast, increased intensity of cytokine immunofluorescence was found in Schwann cells distal to crush lesion. The cytokinepositive Schwann cells were found in close contact with growing axons detected by immunostaining for GAP43. The results of immunohistochemical analysis distal to nerve crush injury suggest that inflammatory profiling of Schwann cells including upregulation of both pro- and anti-inflammatory cytokines does not prevent growth of axons distal to nerve crush injury.

Links

ED1.1.00/02.0068, research and development project
Name: CEITEC - central european institute of technology
286154, interní kód MU
Name: SYLICA - Synergies of Life and Material Sciences to Create a New Future (Acronym: SYLICA)
Investor: European Union, Capacities