Detailed Information on Publication Record
2014
Inflammatory Profiling of Schwann Cells in Contact with Growing Axons Distal to Nerve Injury
DUBOVÝ, Petr, Ilona KLUSÁKOVÁ and Ivana HRADILOVÁ SVÍŽENSKÁBasic information
Original name
Inflammatory Profiling of Schwann Cells in Contact with Growing Axons Distal to Nerve Injury
Authors
DUBOVÝ, Petr (203 Czech Republic, guarantor, belonging to the institution), Ilona KLUSÁKOVÁ (203 Czech Republic, belonging to the institution) and Ivana HRADILOVÁ SVÍŽENSKÁ (203 Czech Republic, belonging to the institution)
Edition
BioMed Research International, USA, Hindawi Publishing Corporation, 2014, 2314-6133
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30000 3. Medical and Health Sciences
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 1.579
RIV identification code
RIV/00216224:14740/14:00078951
Organization unit
Central European Institute of Technology
UT WoS
000335836400001
Keywords in English
activated Schwann cell; growing axons; reinnervation; inflammatory mediators
Tags
Tags
International impact, Reviewed
Změněno: 17/4/2015 09:09, Martina Prášilová
Abstract
V originále
Activated Schwann cells distal to nerve injury upregulate inflammatory mediators, including cytokines. The goal of the present study was to investigate expression of proinflammatory (IL-1b, TNFa) and anti-inflammatory cytokines (IL-4, IL-10) in activated Schwann cells in relation to growing axons distal to crush injury of rat sciatic nerves. Seven days fromsciatic nerve crush, transverse cryostat sections were cut 5 mm distal to lesion and incubated for double immunostaining to indicate Schwann cells (GFAP or S100b) and individual investigated cytokines or to demonstrate growing axons (GAP43). The Schwann cells of naive sciatic nerves and those removed from sham-operated rats displayed similar weak immunoreactivity for the investigated cytokines. In contrast, increased intensity of cytokine immunofluorescence was found in Schwann cells distal to crush lesion. The cytokinepositive Schwann cells were found in close contact with growing axons detected by immunostaining for GAP43. The results of immunohistochemical analysis distal to nerve crush injury suggest that inflammatory profiling of Schwann cells including upregulation of both pro- and anti-inflammatory cytokines does not prevent growth of axons distal to nerve crush injury.
Links
ED1.1.00/02.0068, research and development project |
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286154, interní kód MU |
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