Inflammatory Profiling of Schwann Cells in Contact with Growing Axons Distal to Nerve Injury

DUBOVÝ, Petr, Ilona KLUSÁKOVÁ and Ivana HRADILOVÁ SVÍŽENSKÁ. Inflammatory Profiling of Schwann Cells in Contact with Growing Axons Distal to Nerve Injury. BioMed Research International. USA: Hindawi Publishing Corporation, vol. 2014, April, p. "nestránkováno", 7 pp. ISSN 2314-6133. doi:10.1155/2014/691041. 2014.

Basic information

• Original name: Inflammatory Profiling of Schwann Cells in Contact with Growing Axons Distal to Nerve Injury
• Authors: DUBOVÝ, Petr (203 Czech Republic, guarantor, belonging to the institution), Ilona KLUSÁKOVÁ (203 Czech Republic, belonging to the institution) and Ivana HRADILOVÁ SVÍŽENSKÁ (203 Czech Republic, belonging to the institution).
• Edition: BioMed Research International, USA, Hindawi Publishing Corporation, 2014, 2314-6133.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 30000 3. Medical and Health Sciences
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 1.579
• RIV identification code: RIV/00216224:14740/14:00078951
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1155/2014/691041
• UT WoS: 000335836400001
• Keywords in English: activated Schwann cell; growing axons; reinnervation; inflammatory mediators
• Tags: kontrola MP, rivok
• Tags: International impact, Reviewed

Abstract

Activated Schwann cells distal to nerve injury upregulate inflammatory mediators, including cytokines. The goal of the present study was to investigate expression of proinflammatory (IL-1b, TNFa) and anti-inflammatory cytokines (IL-4, IL-10) in activated Schwann cells in relation to growing axons distal to crush injury of rat sciatic nerves. Seven days fromsciatic nerve crush, transverse cryostat sections were cut 5 mm distal to lesion and incubated for double immunostaining to indicate Schwann cells (GFAP or S100b) and individual investigated cytokines or to demonstrate growing axons (GAP43). The Schwann cells of naive sciatic nerves and those removed from sham-operated rats displayed similar weak immunoreactivity for the investigated cytokines. In contrast, increased intensity of cytokine immunofluorescence was found in Schwann cells distal to crush lesion. The cytokinepositive Schwann cells were found in close contact with growing axons detected by immunostaining for GAP43. The results of immunohistochemical analysis distal to nerve crush injury suggest that inflammatory profiling of Schwann cells including upregulation of both pro- and anti-inflammatory cytokines does not prevent growth of axons distal to nerve crush injury.

Links

• ED1.1.00/02.0068, research and development project: Name: CEITEC - central european institute of technology
• 286154, interní kód MU: Name: SYLICA - Synergies of Life and Material Sciences to Create a New Future (Acronym: SYLICA)

Investor: European Union, Capacities