The ATRA-induced differentiation of medulloblastoma cells is
enhanced with LOX/COX inhibitors: an analysis of gene
expression.

J 2014

The ATRA-induced differentiation of medulloblastoma cells is enhanced with LOX/COX inhibitors: an analysis of gene expression.

CHLAPEK, Petr, Jakub NERADIL, Martina LOJOVÁ, Karel ZITTERBART, Jaroslav ŠTĚRBA et. al.

Basic information

Original name

The ATRA-induced differentiation of medulloblastoma cells is enhanced with LOX/COX inhibitors: an analysis of gene expression.

Authors

CHLAPEK, Petr (203 Czech Republic, belonging to the institution), Jakub NERADIL (203 Czech Republic, belonging to the institution), Martina LOJOVÁ (203 Czech Republic, belonging to the institution), Karel ZITTERBART (203 Czech Republic, belonging to the institution), Jaroslav ŠTĚRBA (203 Czech Republic, belonging to the institution) and Renata VESELSKÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Cancer Cell International, 2014, 1475-2867

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.766

RIV identification code

RIV/00216224:14310/14:00079004

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1186/1475-2867-14-51

UT WoS

000338146500001

Keywords in English

all-trans retinoic acid; caffeic acid; celecoxib; medullobastoma; LOX and COX inhibitors

Abstract

V originále

A detailed analysis of the expression of 440 cancer-related genes was performed after the combined treatment of medulloblastoma cells with all-trans retinoic acid (ATRA) and inhibitors of lipoxygenases (LOX) and cyclooxygenases (COX). The Daoy and D283 Med medulloblastoma cell lines were chosen for this study. Caffeic acid (an inhibitor of 5-LOX) and celecoxib (an inhibitor on COX-2) were used in combined treatment with ATRA. The results clearly confirmed that the differentiating effects of ATRA should be enhanced via its combined administration with caffeic acid or celecoxib. An increased expression of the genes that encoded the proteins participating in induced differentiation and cytoskeleton remodeling was detected in both cell lines in a concentration-dependent manner. This effect was also observed for the CDKN1A gene encoding the p21 protein, which is an important regulator of the cell cycle, and for the genes encoding proteins that are associated with proteasome activity. Furthermore, our results showed that D283 Med cells are significantly more sensitive to treatment with ATRA alone than Daoy cells.

Links

EE2.3.20.0183, research and development project

Name: Centrum experimentální biomedicíny

NR9341, research and development project

Name: Modulace antineoplastického efektu retinoidů LOX/COX inhibitory na modelu dětských solidních nádorů: in vitro studie

Investor: Ministry of Health of the CR

Citovat

CHLAPEK, Petr, Jakub NERADIL, Martina LOJOVÁ, Karel ZITTERBART, Jaroslav ŠTĚRBA and Renata VESELSKÁ. The ATRA-induced differentiation of medulloblastoma cells is enhanced with LOX/COX inhibitors: an analysis of gene expression. Cancer Cell International. 2014, vol. 14, June, p. "nestránkováno", 10 pp. ISSN 1475-2867. Available from: https://dx.doi.org/10.1186/1475-2867-14-51.

@article{1225452,
   author = {Chlapek, Petr and Neradil, Jakub and Lojová, Martina and Zitterbart, Karel and Štěrba, Jaroslav and Veselská, Renata},
   article_number = {June},
   doi = {http://dx.doi.org/10.1186/1475-2867-14-51},
   keywords = {all-trans retinoic acid; caffeic acid; celecoxib; medullobastoma; LOX and COX inhibitors},
   language = {eng},
   issn = {1475-2867},
   journal = {Cancer Cell International},
   title = {The ATRA-induced differentiation of medulloblastoma cells is enhanced with LOX/COX inhibitors: an analysis of gene expression.},
   url = {http://www.cancerci.com/content/14/1/51},
   volume = {14},
   year = {2014}
}

TY  - JOUR
ID  - 1225452
AU  - Chlapek, Petr - Neradil, Jakub - Lojová, Martina - Zitterbart, Karel - Štěrba, Jaroslav - Veselská, Renata
PY  - 2014
TI  - The ATRA-induced differentiation of medulloblastoma cells is enhanced with LOX/COX inhibitors: an analysis of gene expression.
JF  - Cancer Cell International
VL  - 14
IS  - June
SP  - "nestránkováno"
EP  - "nestránkováno"
SN  - 14752867
KW  - all-trans retinoic acid
KW  - caffeic acid
KW  - celecoxib
KW  - medullobastoma
KW  - LOX and COX inhibitors
UR  - http://www.cancerci.com/content/14/1/51
L2  - http://www.cancerci.com/content/14/1/51
N2  - A detailed analysis of the expression of 440 cancer-related genes was performed after the combined treatment of medulloblastoma cells with all-trans retinoic acid (ATRA) and inhibitors of lipoxygenases (LOX) and cyclooxygenases (COX). The Daoy and D283 Med medulloblastoma cell lines were chosen for this study. Caffeic acid (an inhibitor of 5-LOX) and celecoxib (an inhibitor on COX-2) were used in combined treatment with ATRA. The results clearly confirmed that the differentiating effects of ATRA should be enhanced via its combined administration with caffeic acid or celecoxib. An increased expression of the genes that encoded the proteins participating in induced differentiation and cytoskeleton remodeling was detected in both cell lines in a concentration-dependent manner. This effect was also observed for the CDKN1A gene encoding the p21 protein, which is an important regulator of the cell cycle, and for the genes encoding proteins that are associated with proteasome activity. Furthermore, our results showed that D283 Med cells are significantly more sensitive to treatment with ATRA alone than Daoy cells.
ER  -

CHLAPEK, Petr, Jakub NERADIL, Martina LOJOVÁ, Karel ZITTERBART, Jaroslav ŠTĚRBA and Renata VESELSKÁ. The ATRA-induced differentiation of medulloblastoma cells is enhanced with LOX/COX inhibitors: an analysis of gene expression. \textit{Cancer Cell International}. 2014, vol.~14, June, p.~''nestránkováno'', 10 pp. ISSN~1475-2867. Available from: https://dx.doi.org/10.1186/1475-2867-14-51.

Detailed Information on Publication Record