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@article{1227546,
author = {Zimmermann, Michal and Kibe, T. and Kabir, S. and de Lange, T.},
article_location = {COLD SPRING HARBOR},
article_number = {22},
doi = {http://dx.doi.org/10.1101/gad.251611.114},
keywords = {telomere; shelterin; TRF1; BLM; replication; G quadruplex},
language = {eng},
issn = {0890-9369},
journal = {GENES & DEVELOPMENT},
title = {TRF1 negotiates TTAGGG repeat-associated replication problems by recruiting the BLM helicase and the TPP1/POT1 repressor of ATR signaling},
url = {http://genesdev.cshlp.org/content/early/2014/10/23/gad.251611.114.full.pdf+html},
volume = {28},
year = {2014}
}
TY - JOUR
ID - 1227546
AU - Zimmermann, Michal - Kibe, T. - Kabir, S. - de Lange, T.
PY - 2014
TI - TRF1 negotiates TTAGGG repeat-associated replication problems by recruiting the BLM helicase and the TPP1/POT1 repressor of ATR signaling
JF - GENES & DEVELOPMENT
VL - 28
IS - 22
SP - 2477-2491
EP - 2477-2491
PB - COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
SN - 08909369
KW - telomere
KW - shelterin
KW - TRF1
KW - BLM
KW - replication
KW - G quadruplex
UR - http://genesdev.cshlp.org/content/early/2014/10/23/gad.251611.114.full.pdf+html
L2 - http://genesdev.cshlp.org/content/early/2014/10/23/gad.251611.114.full.pdf+html
N2 - The semiconservative replication of telomeres is facilitated by the shelterin component TRF1. Without TRF1, replication forks stall in the telomeric repeats, leading to ATR kinase signaling upon S-phase progression, fragile metaphase telomeres that resemble the common fragile sites (CFSs), and the association of sister telomeres. In contrast, TRF1 does not contribute significantly to the end protection functions of shelterin. We addressed the mechanism of TRF1 action using mouse conditional knockouts of BLM, TRF1, TPP1, and Rap1 in combination with expression of TRF1 and TIN2 mutants. The data establish that TRF1 binds BLM to facilitate lagging but not leading strand telomeric DNA synthesis. As the template for lagging strand telomeric DNA synthesis is the TTAGGG repeat strand, TRF1-bound BLM is likely required to remove secondary structures formed by these sequences. In addition, the data establish that TRF1 deploys TIN2 and the TPP1/POT1 heterodimers in shelterin to prevent ATR during telomere replication and repress the accompanying sister telomere associations. Thus, TRF1 uses two distinct mechanisms to promote replication of telomeric DNA and circumvent the consequences of replication stress. These data are relevant to the expression of CFSs and provide insights into TIN2, which is compromised in dyskeratosis congenita (DC) and related disorders.
ER -
ZIMMERMANN, Michal, T. KIBE, S. KABIR and T. DE LANGE. TRF1 negotiates TTAGGG repeat-associated replication problems by recruiting the BLM helicase and the TPP1/POT1 repressor of ATR signaling. \textit{GENES \&{}amp; DEVELOPMENT}. COLD SPRING HARBOR: COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT, 2014, vol.~28, No~22, p.~2477-2491. ISSN~0890-9369. Available from: https://dx.doi.org/10.1101/gad.251611.114.
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