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@article{1227557,
author = {Muchová, Veronika and Amiard, S. and Mozgová, Iva and Dvořáčková, Martina and Gallego, M. E. and White, Ch. and Fajkus, Jiří},
article_location = {Hoboken (USA)},
article_number = {2},
doi = {http://dx.doi.org/10.1111/tpj.12718},
keywords = {DNA repair; genome instability; 45S rDNA; chromatin assembly factor 1; Arabidopsis thaliana; FAS1; FAS2; RAD51B},
language = {eng},
issn = {0960-7412},
journal = {Plant Journal},
title = {Homology-dependent repair is involved in 45S rDNA loss in plant CAF-1 mutants},
url = {http://onlinelibrary.wiley.com/doi/10.1111/tpj.12718/epdf},
volume = {81},
year = {2015}
}
TY - JOUR
ID - 1227557
AU - Muchová, Veronika - Amiard, S. - Mozgová, Iva - Dvořáčková, Martina - Gallego, M. E. - White, Ch. - Fajkus, Jiří
PY - 2015
TI - Homology-dependent repair is involved in 45S rDNA loss in plant CAF-1 mutants
JF - Plant Journal
VL - 81
IS - 2
SP - 198-209
EP - 198-209
PB - Wiley-Blackwell
SN - 09607412
KW - DNA repair
KW - genome instability
KW - 45S rDNA
KW - chromatin assembly factor 1
KW - Arabidopsis thaliana
KW - FAS1
KW - FAS2
KW - RAD51B
UR - http://onlinelibrary.wiley.com/doi/10.1111/tpj.12718/epdf
L2 - http://onlinelibrary.wiley.com/doi/10.1111/tpj.12718/epdf
N2 - Arabidopsis thaliana mutants in FAS1 and FAS2 subunits of chromatin assembly factor 1 (CAF1) show progressive loss of 45S rDNA copies and telomeres. We hypothesized that homology-dependent DNA damage repair (HDR) may contribute to the loss of these repeats in fas mutants. To test this, we generated double mutants by crossing fas mutants with knock-out mutants in RAD51B, one of the Rad51 paralogs of A.thaliana. Our results show that the absence of RAD51B decreases the rate of rDNA loss, confirming the implication of RAD51B-dependent recombination in rDNA loss in the CAF1 mutants. Interestingly, this effect is not observed for telomeric repeat loss, which thus differs from that acting in rDNA loss. Involvement of DNA damage repair in rDNA dynamics in fas mutants is further supported by accumulation of double-stranded breaks (measured as -H2AX foci) in 45S rDNA. Occurrence of the foci is not specific for S-phase, and is ATM-independent. While the foci in fas mutants occur both in the transcribed (intranucleolar) and non-transcribed (nucleoplasmic) fraction of rDNA, double fas rad51b mutants show a specific increase in the number of the intranucleolar foci. These results suggest that the repair of double-stranded breaks present in the transcribed rDNA region is RAD51B dependent and that this contributes to rDNA repeat loss in fas mutants, presumably via the single-stranded annealing recombination pathway. Our results also highlight the importance of proper chromatin assembly in the maintenance of genome stability. Significance Statement In this work we demonstrate that homology-dependent repair is involved in the observed loss of 45S rDNA in plant CAF-1 mutants. The results suggest that the repair of double-strand breaks present in the transcribed rDNA region is RAD51B-dependent and that this contributes to rDNA repeat loss in fas mutants, presumably via the single strand annealing recombination pathway.
ER -
MUCHOVÁ, Veronika, S. AMIARD, Iva MOZGOVÁ, Martina DVOŘÁČKOVÁ, M. E. GALLEGO, Ch. WHITE a Jiří FAJKUS. Homology-dependent repair is involved in 45S rDNA loss in plant CAF-1 mutants. \textit{Plant Journal}. Hoboken (USA): Wiley-Blackwell, 2015, roč.~81, č.~2, s.~198-209. ISSN~0960-7412. doi:10.1111/tpj.12718.
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