MUCHOVÁ, Veronika, S. AMIARD, Iva MOZGOVÁ, Martina DVOŘÁČKOVÁ, M. E. GALLEGO, Ch. WHITE and Jiří FAJKUS. Homology-dependent repair is involved in 45S rDNA loss in plant CAF-1 mutants. Plant Journal. Hoboken (USA): Wiley-Blackwell, 2015, vol. 81, No 2, p. 198-209. ISSN 0960-7412. doi:10.1111/tpj.12718.
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Basic information
Original name Homology-dependent repair is involved in 45S rDNA loss in plant CAF-1 mutants
Authors MUCHOVÁ, Veronika (203 Czech Republic, belonging to the institution), S. AMIARD (250 France), Iva MOZGOVÁ (203 Czech Republic, belonging to the institution), Martina DVOŘÁČKOVÁ (203 Czech Republic, belonging to the institution), M. E. GALLEGO (250 France), Ch. WHITE (250 France) and Jiří FAJKUS (203 Czech Republic, guarantor, belonging to the institution).
Edition Plant Journal, Hoboken (USA), Wiley-Blackwell, 2015, 0960-7412.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 5.468
RIV identification code RIV/00216224:14740/15:00080676
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1111/tpj.12718
UT WoS 000347830900002
Keywords in English DNA repair; genome instability; 45S rDNA; chromatin assembly factor 1; Arabidopsis thaliana; FAS1; FAS2; RAD51B
Tags podil, rivok
Tags International impact, Reviewed
Changed by Changed by: Olga Křížová, učo 56639. Changed: 4. 5. 2016 10:53.
Abstract
Arabidopsis thaliana mutants in FAS1 and FAS2 subunits of chromatin assembly factor 1 (CAF1) show progressive loss of 45S rDNA copies and telomeres. We hypothesized that homology-dependent DNA damage repair (HDR) may contribute to the loss of these repeats in fas mutants. To test this, we generated double mutants by crossing fas mutants with knock-out mutants in RAD51B, one of the Rad51 paralogs of A.thaliana. Our results show that the absence of RAD51B decreases the rate of rDNA loss, confirming the implication of RAD51B-dependent recombination in rDNA loss in the CAF1 mutants. Interestingly, this effect is not observed for telomeric repeat loss, which thus differs from that acting in rDNA loss. Involvement of DNA damage repair in rDNA dynamics in fas mutants is further supported by accumulation of double-stranded breaks (measured as -H2AX foci) in 45S rDNA. Occurrence of the foci is not specific for S-phase, and is ATM-independent. While the foci in fas mutants occur both in the transcribed (intranucleolar) and non-transcribed (nucleoplasmic) fraction of rDNA, double fas rad51b mutants show a specific increase in the number of the intranucleolar foci. These results suggest that the repair of double-stranded breaks present in the transcribed rDNA region is RAD51B dependent and that this contributes to rDNA repeat loss in fas mutants, presumably via the single-stranded annealing recombination pathway. Our results also highlight the importance of proper chromatin assembly in the maintenance of genome stability. Significance Statement In this work we demonstrate that homology-dependent repair is involved in the observed loss of 45S rDNA in plant CAF-1 mutants. The results suggest that the repair of double-strand breaks present in the transcribed rDNA region is RAD51B-dependent and that this contributes to rDNA repeat loss in fas mutants, presumably via the single strand annealing recombination pathway.
Links
ED1.1.00/02.0068, research and development projectName: CEITEC - central european institute of technology
EE2.3.30.0009, research and development projectName: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci
GAP501/11/0289, research and development projectName: Ztráta specifických repetic DNA v důsledku dysfunkce CAF1 u rostlin
Investor: Czech Science Foundation
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