Characterization of DNA Repair Deficient Strains of
Chlamydomonas reinhardtii Generated by Insertional Mutagenesis

J 2014

Characterization of DNA Repair Deficient Strains of Chlamydomonas reinhardtii Generated by Insertional Mutagenesis

PLECENIKOVA, A., M. SLANINOVA and Karel ŘÍHA

Basic information

Original name

Characterization of DNA Repair Deficient Strains of Chlamydomonas reinhardtii Generated by Insertional Mutagenesis

Authors

PLECENIKOVA, A. (703 Slovakia), M. SLANINOVA (703 Slovakia) and Karel ŘÍHA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Plos one, SAN FRANCISCO, PUBLIC LIBRARY SCIENCE, 2014, 1932-6203

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.234

RIV identification code

RIV/00216224:14740/14:00079173

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1371/journal.pone.0105482

UT WoS

000341106100073

Keywords in English

HOMOLOGOUS RECOMBINATION; NUCLEAR TRANSFORMATION; HUMAN CTIP; ARG7 GENE; ARABIDOPSIS; PLANTS; GENOME; DAMAGE; MECHANISMS; RESPONSES

Abstract

V originále

While the mechanisms governing DNA damage response and repair are fundamentally conserved, cross-kingdom comparisons indicate that they differ in many aspects due to differences in life-styles and developmental strategies. In photosynthetic organisms these differences have not been fully explored because gene-discovery approaches are mainly based on homology searches with known DDR/DNA repair proteins. Here we performed a forward genetic screen in the green algae Chlamydomonas reinhardtii to identify genes deficient in DDR/DNA repair. We isolated five insertional mutants that were sensitive to various genotoxic insults and two of them exhibited altered efficiency of transgene integration. To identify genomic regions disrupted in these mutants, we established a novel adaptor-ligation strategy for the efficient recovery of the insertion flanking sites. Four mutants harbored deletions that involved known DNA repair factors, DNA Pol zeta, DNA Pol theta, SAE2/COM1, and two neighbouring genes encoding ERCC1 and RAD17. Deletion in the last mutant spanned two Chlamydomonas-specific genes with unknown function, demonstrating the utility of this approach for discovering novel factors involved in genome maintenance.

Citovat

PLECENIKOVA, A., M. SLANINOVA and Karel ŘÍHA. Characterization of DNA Repair Deficient Strains of Chlamydomonas reinhardtii Generated by Insertional Mutagenesis. Plos one. SAN FRANCISCO: PUBLIC LIBRARY SCIENCE, 2014, vol. 9, No 8, p. "nestránkováno", 9 pp. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0105482.

@article{1227576,
   author = {Plecenikova, A. and Slaninova, M. and Říha, Karel},
   article_location = {SAN FRANCISCO},
   article_number = {8},
   doi = {http://dx.doi.org/10.1371/journal.pone.0105482},
   keywords = {HOMOLOGOUS RECOMBINATION; NUCLEAR TRANSFORMATION; HUMAN CTIP; ARG7 GENE; ARABIDOPSIS; PLANTS; GENOME; DAMAGE; MECHANISMS; RESPONSES},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {Characterization of DNA Repair Deficient Strains of Chlamydomonas reinhardtii Generated by Insertional Mutagenesis},
   url = {http://www.plosone.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pone.0105482&representation=PDF},
   volume = {9},
   year = {2014}
}

TY  - JOUR
ID  - 1227576
AU  - Plecenikova, A. - Slaninova, M. - Říha, Karel
PY  - 2014
TI  - Characterization of DNA Repair Deficient Strains of Chlamydomonas reinhardtii Generated by Insertional Mutagenesis
JF  - Plos one
VL  - 9
IS  - 8
SP  - "nestránkováno"
EP  - "nestránkováno"
PB  - PUBLIC LIBRARY SCIENCE
SN  - 19326203
KW  - HOMOLOGOUS RECOMBINATION
KW  - NUCLEAR TRANSFORMATION
KW  - HUMAN CTIP
KW  - ARG7 GENE
KW  - ARABIDOPSIS
KW  - PLANTS
KW  - GENOME
KW  - DAMAGE
KW  - MECHANISMS
KW  - RESPONSES
UR  - http://www.plosone.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pone.0105482&representation=PDF
L2  - http://www.plosone.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pone.0105482&representation=PDF
N2  - While the mechanisms governing DNA damage response and repair are fundamentally conserved, cross-kingdom comparisons indicate that they differ in many aspects due to differences in life-styles and developmental strategies. In photosynthetic organisms these differences have not been fully explored because gene-discovery approaches are mainly based on homology searches with known DDR/DNA repair proteins. Here we performed a forward genetic screen in the green algae Chlamydomonas reinhardtii to identify genes deficient in DDR/DNA repair. We isolated five insertional mutants that were sensitive to various genotoxic insults and two of them exhibited altered efficiency of transgene integration. To identify genomic regions disrupted in these mutants, we established a novel adaptor-ligation strategy for the efficient recovery of the insertion flanking sites. Four mutants harbored deletions that involved known DNA repair factors, DNA Pol zeta, DNA Pol theta, SAE2/COM1, and two neighbouring genes encoding ERCC1 and RAD17. Deletion in the last mutant spanned two Chlamydomonas-specific genes with unknown function, demonstrating the utility of this approach for discovering novel factors involved in genome maintenance.
ER  -

PLECENIKOVA, A., M. SLANINOVA and Karel ŘÍHA. Characterization of DNA Repair Deficient Strains of Chlamydomonas reinhardtii Generated by Insertional Mutagenesis. \textit{Plos one}. SAN FRANCISCO: PUBLIC LIBRARY SCIENCE, 2014, vol.~9, No~8, p.~''nestránkováno'', 9 pp. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0105482.

Detailed Information on Publication Record