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@article{1227576,
author = {Plecenikova, A. and Slaninova, M. and Říha, Karel},
article_location = {SAN FRANCISCO},
article_number = {8},
doi = {http://dx.doi.org/10.1371/journal.pone.0105482},
keywords = {HOMOLOGOUS RECOMBINATION; NUCLEAR TRANSFORMATION; HUMAN CTIP; ARG7 GENE; ARABIDOPSIS; PLANTS; GENOME; DAMAGE; MECHANISMS; RESPONSES},
language = {eng},
issn = {1932-6203},
journal = {Plos one},
title = {Characterization of DNA Repair Deficient Strains of Chlamydomonas reinhardtii Generated by Insertional Mutagenesis},
url = {http://www.plosone.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pone.0105482&representation=PDF},
volume = {9},
year = {2014}
}
TY - JOUR
ID - 1227576
AU - Plecenikova, A. - Slaninova, M. - Říha, Karel
PY - 2014
TI - Characterization of DNA Repair Deficient Strains of Chlamydomonas reinhardtii Generated by Insertional Mutagenesis
JF - Plos one
VL - 9
IS - 8
SP - "nestránkováno"
EP - "nestránkováno"
PB - PUBLIC LIBRARY SCIENCE
SN - 19326203
KW - HOMOLOGOUS RECOMBINATION
KW - NUCLEAR TRANSFORMATION
KW - HUMAN CTIP
KW - ARG7 GENE
KW - ARABIDOPSIS
KW - PLANTS
KW - GENOME
KW - DAMAGE
KW - MECHANISMS
KW - RESPONSES
UR - http://www.plosone.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pone.0105482&representation=PDF
L2 - http://www.plosone.org/article/fetchObject.action?uri=info:doi/10.1371/journal.pone.0105482&representation=PDF
N2 - While the mechanisms governing DNA damage response and repair are fundamentally conserved, cross-kingdom comparisons indicate that they differ in many aspects due to differences in life-styles and developmental strategies. In photosynthetic organisms these differences have not been fully explored because gene-discovery approaches are mainly based on homology searches with known DDR/DNA repair proteins. Here we performed a forward genetic screen in the green algae Chlamydomonas reinhardtii to identify genes deficient in DDR/DNA repair. We isolated five insertional mutants that were sensitive to various genotoxic insults and two of them exhibited altered efficiency of transgene integration. To identify genomic regions disrupted in these mutants, we established a novel adaptor-ligation strategy for the efficient recovery of the insertion flanking sites. Four mutants harbored deletions that involved known DNA repair factors, DNA Pol zeta, DNA Pol theta, SAE2/COM1, and two neighbouring genes encoding ERCC1 and RAD17. Deletion in the last mutant spanned two Chlamydomonas-specific genes with unknown function, demonstrating the utility of this approach for discovering novel factors involved in genome maintenance.
ER -
PLECENIKOVA, A., M. SLANINOVA and Karel ŘÍHA. Characterization of DNA Repair Deficient Strains of Chlamydomonas reinhardtii Generated by Insertional Mutagenesis. \textit{Plos one}. SAN FRANCISCO: PUBLIC LIBRARY SCIENCE, 2014, vol.~9, No~8, p.~''nestránkováno'', 9 pp. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0105482.
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