Detailed Information on Publication Record
2014
Dynamic infrared imaging analysis of apical hook development in Arabidopsis: the case of brassinosteroids
SMET, D., P. ŽÁDNÍKOVÁ, F. VANDENBUSSCHE, Eva FRIMLOVÁ, D. VAN DER STRAETEN et. al.Basic information
Original name
Dynamic infrared imaging analysis of apical hook development in Arabidopsis: the case of brassinosteroids
Authors
SMET, D. (56 Belgium), P. ŽÁDNÍKOVÁ (56 Belgium), F. VANDENBUSSCHE (56 Belgium), Eva FRIMLOVÁ (703 Slovakia, guarantor, belonging to the institution) and D. VAN DER STRAETEN (56 Belgium)
Edition
New Phytologist, HOBOKEN, WILEY-BLACKWELL, 2014, 0028-646X
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 7.672
RIV identification code
RIV/00216224:14740/14:00079200
Organization unit
Central European Institute of Technology
UT WoS
000335470200029
Keywords in English
apical hook; Arabidopsis thaliana; brassinosteroids (BRs); cross-talk; ethylene; infrared imaging; kinematics; light
Tags
Tags
International impact, Reviewed
Změněno: 24/2/2015 11:29, Martina Prášilová
Abstract
V originále
Germination of Arabidopsis seeds in darkness induces apical hook development, based on a tightly regulated differential growth coordinated by a multiple hormone cross-talk. Here, we endeavoured to clarify the function of brassinosteroids (BRs) and cross-talk with ethylene in hook development. An automated infrared imaging system was developed to study the kinetics of hook development in etiolated Arabidopsis seedlings. To ascertain the photomorphogenic control of hook opening, the system was equipped with an automatic light dimmer. We demonstrate that ethylene and BRs are indispensable for hook formation and maintenance. Ethylene regulation of hook formation functions partly through BRs, with BR feedback inhibition of ethylene action. Conversely, BR-mediated extension of hook maintenance functions partly through ethylene. Furthermore, we revealed that a short light pulse is sufficient to induce rapid hook opening. Our dynamic infrared imaging system allows high-resolution, kinetic imaging of up to 112 seedlings in a single experimental run. At this high throughput, it is ideally suited to rapidly gain insight in pathway networks. We demonstrate that BRs and ethylene cooperatively regulate apical hook development in a phase-dependent manner. Furthermore, we show that light is a predominant regulator of hook opening, inhibiting ethylene- and BR-mediated postponement of hook opening.
Links
| EE2.3.20.0043, research and development project |
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