A virus capsid-like nanocompartment that stores iron and
protects bacteria from oxidative stress

J 2014

A virus capsid-like nanocompartment that stores iron and protects bacteria from oxidative stress

MCHUGH, C.A., J. FONTANA, Daniel NĚMEČEK, N.Q. CHENG, A.A. AKSYUK et. al.

Basic information

Original name

A virus capsid-like nanocompartment that stores iron and protects bacteria from oxidative stress

Authors

MCHUGH, C.A. (840 United States of America), J. FONTANA (840 United States of America), Daniel NĚMEČEK (203 Czech Republic, guarantor, belonging to the institution), N.Q. CHENG (840 United States of America), A.A. AKSYUK (840 United States of America), J.B. HEYMANN (840 United States of America), D.C. WINKLER (840 United States of America), A.S. LAM (840 United States of America), J.S. WALL (840 United States of America), A.C. STEVEN (840 United States of America) and E. HOICZYK (840 United States of America)

Edition

EMBO Journal, HOBOKEN, WILEY-BLACKWELL, 2014, 0261-4189

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 10.434

RIV identification code

RIV/00216224:14740/14:00079216

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.15252/embj.201488566

UT WoS

000341839500008

Keywords in English

cryo-electron microscopy; encapsulin; ferritin; HK97 fold; oxidative stress

Abstract

V originále

Living cells compartmentalize materials and enzymatic reactions to increase metabolic efficiency. While eukaryotes use membrane-bound organelles, bacteria and archaea rely primarily on protein-bound nanocompartments. Encapsulins constitute a class of nanocompartments widespread in bacteria and archaea whose functions have hitherto been unclear. Here, we characterize the encapsulin nanocompartment from Myxococcus xanthus, which consists of a shell protein (EncA, 32.5 kDa) and three internal proteins (EncB, 17 kDa; EncC, 13 kDa; EncD, 11 kDa). Using cryo-electron microscopy, we determined that EncA self-assembles into an icosahedral shell 32 nm in diameter (26 nm internal diameter), built from 180 subunits with the fold first observed in bacteriophage HK97 capsid. The internal proteins, of which EncB and EncC have ferritin-like domains, attach to its inner surface. Native nanocompartments have dense iron-rich cores. Functionally, they resemble ferritins, cage-like iron storage proteins, but with a massively greater capacity (similar to 30,000 iron atoms versus similar to 3,000 in ferritin). Physiological data reveal that few nanocompartments are assembled during vegetative growth, but they increase fivefold upon starvation, protecting cells from oxidative stress through iron sequestration.

Citovat

MCHUGH, C.A., J. FONTANA, Daniel NĚMEČEK, N.Q. CHENG, A.A. AKSYUK, J.B. HEYMANN, D.C. WINKLER, A.S. LAM, J.S. WALL, A.C. STEVEN and E. HOICZYK. A virus capsid-like nanocompartment that stores iron and protects bacteria from oxidative stress. EMBO Journal. HOBOKEN: WILEY-BLACKWELL, 2014, vol. 33, No 17, p. 1896-1911. ISSN 0261-4189. Available from: https://dx.doi.org/10.15252/embj.201488566.

@article{1227817,
   author = {McHugh, C.A. and Fontana, J. and Němeček, Daniel and Cheng, N.Q. and Aksyuk, A.A. and Heymann, J.B. and Winkler, D.C. and Lam, A.S. and Wall, J.S. and Steven, A.C. and Hoiczyk, E.},
   article_location = {HOBOKEN},
   article_number = {17},
   doi = {http://dx.doi.org/10.15252/embj.201488566},
   keywords = {cryo-electron microscopy; encapsulin; ferritin; HK97 fold; oxidative stress},
   language = {eng},
   issn = {0261-4189},
   journal = {EMBO Journal},
   title = {A virus capsid-like nanocompartment that stores iron and protects bacteria from oxidative stress},
   url = {http://emboj.embopress.org/content/embojnl/33/17/1896.full.pdf},
   volume = {33},
   year = {2014}
}

TY  - JOUR
ID  - 1227817
AU  - McHugh, C.A. - Fontana, J. - Němeček, Daniel - Cheng, N.Q. - Aksyuk, A.A. - Heymann, J.B. - Winkler, D.C. - Lam, A.S. - Wall, J.S. - Steven, A.C. - Hoiczyk, E.
PY  - 2014
TI  - A virus capsid-like nanocompartment that stores iron and protects bacteria from oxidative stress
JF  - EMBO Journal
VL  - 33
IS  - 17
SP  - 1896-1911
EP  - 1896-1911
PB  - WILEY-BLACKWELL
SN  - 02614189
KW  - cryo-electron microscopy
KW  - encapsulin
KW  - ferritin
KW  - HK97 fold
KW  - oxidative stress
UR  - http://emboj.embopress.org/content/embojnl/33/17/1896.full.pdf
L2  - http://emboj.embopress.org/content/embojnl/33/17/1896.full.pdf
N2  - Living cells compartmentalize materials and enzymatic reactions to increase metabolic efficiency. While eukaryotes use membrane-bound organelles, bacteria and archaea rely primarily on protein-bound nanocompartments. Encapsulins constitute a class of nanocompartments widespread in bacteria and archaea whose functions have hitherto been unclear. Here, we characterize the encapsulin nanocompartment from Myxococcus xanthus, which consists of a shell protein (EncA, 32.5 kDa) and three internal proteins (EncB, 17 kDa; EncC, 13 kDa; EncD, 11 kDa). Using cryo-electron microscopy, we determined that EncA self-assembles into an icosahedral shell 32 nm in diameter (26 nm internal diameter), built from 180 subunits with the fold first observed in bacteriophage HK97 capsid. The internal proteins, of which EncB and EncC have ferritin-like domains, attach to its inner surface. Native nanocompartments have dense iron-rich cores. Functionally, they resemble ferritins, cage-like iron storage proteins, but with a massively greater capacity (similar to 30,000 iron atoms versus similar to 3,000 in ferritin). Physiological data reveal that few nanocompartments are assembled during vegetative growth, but they increase fivefold upon starvation, protecting cells from oxidative stress through iron sequestration.
ER  -

MCHUGH, C.A., J. FONTANA, Daniel NĚMEČEK, N.Q. CHENG, A.A. AKSYUK, J.B. HEYMANN, D.C. WINKLER, A.S. LAM, J.S. WALL, A.C. STEVEN and E. HOICZYK. A virus capsid-like nanocompartment that stores iron and protects bacteria from oxidative stress. \textit{EMBO Journal}. HOBOKEN: WILEY-BLACKWELL, 2014, vol.~33, No~17, p.~1896-1911. ISSN~0261-4189. Available from: https://dx.doi.org/10.15252/embj.201488566.

Detailed Information on Publication Record