Detailed Information on Publication Record
2014
Acinetobacter gandensis sp nov isolated from horse and cattle
SMET, Annemieke, Piet COOLS, Lenka KŘÍŽOVÁ, Martina MAIXNEROVÁ, Ondrej ŠEDO et. al.Basic information
Original name
Acinetobacter gandensis sp nov isolated from horse and cattle
Authors
SMET, Annemieke (56 Belgium), Piet COOLS (56 Belgium), Lenka KŘÍŽOVÁ (203 Czech Republic), Martina MAIXNEROVÁ (203 Czech Republic), Ondrej ŠEDO (203 Czech Republic, guarantor, belonging to the institution), Freddy HAESEBROUCK (56 Belgium), Marie KEMPF (250 France), Alexandr NEMEC (203 Czech Republic) and Mario VANEECHOUTTE (56 Belgium)
Edition
International Journal of Systematic and Evolutionary Microbiology, Berks (England), Soc General Microbiology, 2014, 1466-5026
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 2.511
RIV identification code
RIV/00216224:14740/14:00074419
Organization unit
Central European Institute of Technology
UT WoS
000348622000014
Keywords in English
Acinetobacter gandensis; taxonomy; rpoB; 16S rRNA gene; MALDI-TOF MS
Tags
Tags
International impact, Reviewed
Změněno: 11/3/2015 12:36, Martina Prášilová
Abstract
V originále
We previously reported the presence of an OXA-23 carbapenemase in an undescribed species of the genus Acinetobacter isolated from horse dung at the Faculty of Veterinary Medicine, Ghent University, Belgium. Here we include six strains to corroborate the delineation of this taxon by phenotypic characterization, DNA-DNA hybridization, 16S rRNA gene and rpoB sequence analysis, % G+C determination, MALDI-TOF MS and fatty acid analysis. The nearly complete 16S rRNA gene sequence of strain UG 60467(T) showed the highest similarities with those of the type strains of Acinetobacter bouvetii (98.4%), Acinetobacter haemolyticus (97.7%), and Acinetobacter schindleri (97.2%). The partial rpoB sequence of strain UG 60467(T) showed the highest similarities with 'Acinetobacter bohemicus' ANC 3994 (88.6%), A. bouvetii NIPH 2281 (88.6%) and A. schindleri CIP 1072871 (87.3%). Whole-cell MALDI-TOF MS analyses supported the distinctness of the group at the protein level. The predominant fatty acids of strain UG 60467(T) were C-12:0 3-OH, C-12:0, C-16:0, C-18:1 omega 9c and summed feature 3 (C-16:1 omega 7c and/or iso-C-15:0 2-OH). Strains UG 60467(T) and UG 60716 showed a DNA-DNA relatedness of 84% with each other and a DNA-DNA relatedness with A. schindleri LMG 19576(T) of 17 % and 20%, respectively. The DNA G C content of strain UG 60467(T) was 39.6 molok. The name Acinetobacter gandensis sp. nov. is proposed for the novel taxon. The type strain is UG 60467(T) (=ANC 4275(T)=LMG 27960(T)=DSM 28097(T)).
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