| NGUYEN, Hoai Viet, Zbynek HEGER, Marketa KOMINKOVA, Petr MICHÁLEK, Jaromír GUMULEC, Roman GURÁŇ, Antonin PRIDAL, Carlos FERNANDEZ, David HYNEK, Vojtěch ADAM a René KIZEK. The Electrochemical and Statistical Evaluation of Isolation of Mellitin and Apamin from Honey Bee (Apis Mellifera) Venom. International Journal of Electrochemical Science. Belgrade: Electrochemical Science Group, roč. 10, č. 2, s. 1249-1260. ISSN 1452-3981. 2015. |
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@article{1228803,
author = {Nguyen, Hoai Viet and Heger, Zbynek and Kominkova, Marketa and Michálek, Petr and Gumulec, Jaromír and Guráň, Roman and Pridal, Antonin and Fernandez, Carlos and Hynek, David and Adam, Vojtěch and Kizek, René},
article_location = {Belgrade},
article_number = {2},
keywords = {antimicrobial peptides; Apis mellifera; Brdicka reaction; MALDI-TOF; Differential pulse voltammetry},
language = {eng},
issn = {1452-3981},
journal = {International Journal of Electrochemical Science},
title = {The Electrochemical and Statistical Evaluation of Isolation of Mellitin and Apamin from Honey Bee (Apis Mellifera) Venom},
url = {http://www.electrochemsci.org/papers/vol10/100201249.pdf},
volume = {10},
year = {2015}
}
TY - JOUR
ID - 1228803
AU - Nguyen, Hoai Viet - Heger, Zbynek - Kominkova, Marketa - Michálek, Petr - Gumulec, Jaromír - Guráň, Roman - Pridal, Antonin - Fernandez, Carlos - Hynek, David - Adam, Vojtěch - Kizek, René
PY - 2015
TI - The Electrochemical and Statistical Evaluation of Isolation of Mellitin and Apamin from Honey Bee (Apis Mellifera) Venom
JF - International Journal of Electrochemical Science
VL - 10
IS - 2
SP - 1249-1260
EP - 1249-1260
PB - Electrochemical Science Group
SN - 14523981
KW - antimicrobial peptides
KW - Apis mellifera
KW - Brdicka reaction
KW - MALDI-TOF
KW - Differential pulse voltammetry
UR - http://www.electrochemsci.org/papers/vol10/100201249.pdf
N2 - We present in this manuscript for the first time the electrochemical and statistical evaluation of FPLC isolation of mellitin and apamin from honey bee (Apis mellifera) venom. Venoms are extremely complex blends of diverse substances that target a myriad of receptors or ion channels. Therefore, toxins, isolated from venomous organisms can be a valuable tool with diverse biological applications. In this study we decided to optimize the purification of honey bee venom by using fast protein liquid chromatography, to obtain biologically active peptide - melittin (2846.46 Da). Due to a presence of other compounds with similar molecular weight (apamin 2027.34 Da), we optimized a differential pulse voltammetry method with adsorptive transfer technique (AdT DPV), utilizing Brdicka supporting electrolyte for measurements. Typical voltammograms - fingerprints for each substance were obtained and numerical projections of voltammograms were employed to propose an artificial neural network. Our suggested neural network can simply predict the content of each peptide in fraction with following performance: 100% for training and 100% for testing.
ER -
NGUYEN, Hoai Viet, Zbynek HEGER, Marketa KOMINKOVA, Petr MICHÁLEK, Jaromír GUMULEC, Roman GURÁŇ, Antonin PRIDAL, Carlos FERNANDEZ, David HYNEK, Vojtěch ADAM a René KIZEK. The Electrochemical and Statistical Evaluation of Isolation of Mellitin and Apamin from Honey Bee (Apis Mellifera) Venom. \textit{International Journal of Electrochemical Science}. Belgrade: Electrochemical Science Group, roč.~10, č.~2, s.~1249-1260. ISSN~1452-3981. 2015.
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