KRÁLOVÁ, Tereza, Radim CEGAN, Zdeněk KUBÁT, Jan VRÁNA, Boris VYSKOT, Ivan VOGEL, Eduard KEJNOVSKÝ and Roman HOBZA. Identification of a Novel Retrotransposon with Sex Chromosome-Specific Distribution in Silene latifolia. Cytogenetic and Genome research. Basel: Karger, 2014, vol. 143, 1-3, p. 87-95. ISSN 1424-8581. Available from: https://dx.doi.org/10.1159/000362142.
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Basic information
Original name Identification of a Novel Retrotransposon with Sex Chromosome-Specific Distribution in Silene latifolia
Authors KRÁLOVÁ, Tereza (203 Czech Republic), Radim CEGAN (203 Czech Republic), Zdeněk KUBÁT (203 Czech Republic), Jan VRÁNA (203 Czech Republic), Boris VYSKOT (203 Czech Republic), Ivan VOGEL (703 Slovakia, belonging to the institution), Eduard KEJNOVSKÝ (203 Czech Republic, guarantor, belonging to the institution) and Roman HOBZA (203 Czech Republic).
Edition Cytogenetic and Genome research, Basel, Karger, 2014, 1424-8581.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher Switzerland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 1.561
RIV identification code RIV/00216224:14740/14:00079375
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1159/000362142
UT WoS 000342614100009
Keywords in English Microdissection; Sex chromosomes; Silene latifolia (white campion); Retrotransposons
Tags kontrola MP, MP, podíl Vavpi, rivok
Tags International impact, Reviewed
Changed by Changed by: Martina Prášilová, učo 342282. Changed: 4/3/2015 15:42.
Abstract
Silene latifolia is a dioecious plant species with chromosomal sex determination. Although the evolution of sex chromosomes in S. latifolia has been the subject of numerous studies, a global view of X chromosome structure in this species is still missing. Here, we combine X chromosome microdissection and BAC library screening to isolate new X chromosome-linked sequences. Out of 8 identified BAC clones, only BAC 86M14 showed an X-preferential signal after FISH experiments. Further analysis revealed the existence of the Athila retroelement which is enriched in the X chromosome and nearly absent in the Y chromosome. Based on previous data, the Athila retroelement belongs to the CL3 group of most repetitive sequences in the S. latifolia genome. Structural, transcriptomics and phylogenetic analyses revealed that Athila CL3 represents an old clade in the Athila lineage. We propose a mechanism responsible for Athila CL3 distribution in the S. latifolia genome. (C) 2014 S. Karger AG, Basel
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