Detailed Information on Publication Record
2015
Endocrine, teratogenic and neurotoxic effects of cyanobacteria detected by cellular in vitro and zebrafish embryos assays
JONÁŠ, Adam, Stefan SCHOLZ, Eva FETTER, Eliška SYCHROVÁ, Kateřina NOVÁKOVÁ et. al.Basic information
Original name
Endocrine, teratogenic and neurotoxic effects of cyanobacteria detected by cellular in vitro and zebrafish embryos assays
Authors
JONÁŠ, Adam (203 Czech Republic, belonging to the institution), Stefan SCHOLZ (276 Germany), Eva FETTER (276 Germany), Eliška SYCHROVÁ (203 Czech Republic, belonging to the institution), Kateřina NOVÁKOVÁ (203 Czech Republic, belonging to the institution), Julia ORTMANN (276 Germany), Martin BENÍŠEK (203 Czech Republic, belonging to the institution), Ondřej ADAMOVSKÝ (203 Czech Republic, belonging to the institution), John P. GIESY (124 Canada) and Klára HILSCHEROVÁ (203 Czech Republic, guarantor, belonging to the institution)
Edition
Chemosphere, OXFORD (ENGLAND), Elsevier Science, 2015, 0045-6535
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10511 Environmental sciences
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 3.698
RIV identification code
RIV/00216224:14310/15:00080689
Organization unit
Faculty of Science
UT WoS
000348003200044
Keywords in English
Estrogenicity; Teratogenicity; Retinoid-like activity; Blue-green algae; Fish
Tags
International impact, Reviewed
Změněno: 6/4/2016 10:03, Ing. Andrea Mikešková
Abstract
V originále
Cyanobacteria contain various types of bioactive compounds, which could cause adverse effects on organisms. They are released into surface waters during cyanobacterial blooms, but there is little information on their potential relevance for effects in vivo. In this study presence of bioactive compounds was characterized in cyanobacteria Microcystis aeruginosa (Chroococcales), Planktothrix agardhii (Oscillatoriales) and Aphanizomenon gracile (Nostocales) with selected in vitro assays. The in vivo relevance of detected bioactivities was analysed using transgenic zebrafish embryos tg(cypi9a1b-GFP). Teratogenic potency was assessed by analysis of developmental disorders and effects on functions of the neuromuscular system by video tracking of locomotion. Estrogenicity in vitro corresponded to 0.95-54.6 ng estradiol equivalent (g dry weight (dw))(-1). In zebrafish embryos, estrogenic effects could not be detected potentially because they were masked by high toxicity. There was no detectable (anti)androgenic/glucocorticoid activity in any sample. Retinoid-like activity was determined at 1-1.3 mu g all-trans-retinoic acid equivalent (g dw)-1. Corresponding to the retinoid-like activity A. gracile extract also caused teratogenic effects in zebrafish embryos. Furthermore, exposure to biomass extracts at 0.3 g dw L-1 caused increase of body length in embryos. There were minor effects on locomotion caused by 0.3 g dw L-1 M. aeruginosa and P. agardhii extracts. The traditionally measured cyanotoxins microcystins did not seem to play significant role in observed effects. This indicates importance of other cyanobacterial compounds at least towards some species or their developmental phases. More attention should be paid to activity of retinoids, estrogens and other bioactive substances in phytoplankton using in vitro and in vivo bioassays.
Links
| GAP503/12/0553, research and development project |
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