Retinoid-like activity and teratogenic effects of
cyanobacterial exudates

J 2014

Retinoid-like activity and teratogenic effects of cyanobacterial exudates

JONÁŠ, Adam; Veronika BURÁŇOVÁ; Stefan SCHOLZ; Eva FETTER; Kateřina NOVÁKOVÁ et. al.

Základní údaje

Originální název

Retinoid-like activity and teratogenic effects of cyanobacterial exudates

Autoři

JONÁŠ, Adam (203 Česká republika, domácí); Veronika BURÁŇOVÁ (203 Česká republika, domácí); Stefan SCHOLZ (276 Německo); Eva FETTER (276 Německo); Kateřina NOVÁKOVÁ (203 Česká republika, domácí); Jiří KOHOUTEK (203 Česká republika, domácí) a Klára HILSCHEROVÁ (203 Česká republika, garant, domácí)

Vydání

Aquatic toxicology, AMSTERDAM, ELSEVIER SCIENCE BV, 2014, 0166-445X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.451

Kód RIV

RIV/00216224:14310/14:00074441

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.aquatox.2014.06.022

UT WoS

000342245300032

EID Scopus

2-s2.0-84905396022

Klíčová slova anglicky

Cyanobacteria; Developmental toxicity; Retinoids; Zebrafish embryo; All-trans retinoic acid

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 4. 2015 12:01, Ing. Andrea Mikešková

Anotace

V originále

Given the essential role of retinoids for vertebrate development this has raised concerns about a potential risk for vertebrates exposed to retinoids during cyanobacterial blooms. Our study focuses on extracellular compounds produced by phytoplankton cells (exudates). In order to address the capacity for the production of retinoids or compounds with retinoid-like activity we compared the exudates of ten cyanobacteria and algae using in vitro reporter gene assay. Exudates of three cyanobacterial species showed retinoid-like activity in the range of 269-2265 ng retinoid equivalents (REQ)/L, while there was no detectable activity in exudates of the investigated algal species. The exudates of one green alga (Desmodesmus quadricaudus) and the two cyanobacterial species with greatest REQ levels, Microgstis aeruginosa and Cylindrospermopsis raciborskii, were selected for testing of the potential relation of retinoid-like activity to developmental toxicity in zebrafish embryos. The exudates of both cyanobacteria were indeed provoking diverse teratogenic effects (e.g. tail, spine and mouth deformation) and interference with growth in zebrafish embryos, while such effects were not observed for the alga. Fish embryos were also exposed to all-trans retinoic acid (ATRA) in a range equivalent to the REQ concentrations detected in exudates by in vitro bioassays. Both the phenotypes and effective concentrations of exudates corresponded to ATRA equivalents, supporting the hypothesis that the teratogenic effects of cyanobacterial exudates are likely to be associated with retinoid-like activity. The study documents that some cyanobacteria are able to produce and release retinoid-like compounds into the environment at concentrations equivalent to those causing teratogenicity in zebrafish. Hence, the characterization of retinoid-like and teratogenic potency should be included in the assessment of the potential adverse effects caused by the release of toxic and bioactive compounds during cyanobacterial blooms.

Návaznosti

GAP503/12/0553, projekt VaV

Název: Metabolity sinic jako potenciální endokrinní disruptory

Investor: Grantová agentura ČR, Metabolity sinic jako potenciální endokrinní disruptory

Citovat

JONÁŠ, Adam; Veronika BURÁŇOVÁ; Stefan SCHOLZ; Eva FETTER; Kateřina NOVÁKOVÁ; Jiří KOHOUTEK a Klára HILSCHEROVÁ. Retinoid-like activity and teratogenic effects of cyanobacterial exudates. Aquatic toxicology. AMSTERDAM: ELSEVIER SCIENCE BV, 2014, roč. 155, OCTOBER, s. 283-290. ISSN 0166-445X. Dostupné z: https://dx.doi.org/10.1016/j.aquatox.2014.06.022.

@article{1229158,
   author = {Jonáš, Adam and Buráňová, Veronika and Scholz, Stefan and Fetter, Eva and Nováková, Kateřina and Kohoutek, Jiří and Hilscherová, Klára},
   article_location = {AMSTERDAM},
   article_number = {OCTOBER},
   doi = {http://dx.doi.org/10.1016/j.aquatox.2014.06.022},
   keywords = {Cyanobacteria; Developmental toxicity; Retinoids; Zebrafish embryo; All-trans retinoic acid},
   language = {eng},
   issn = {0166-445X},
   journal = {Aquatic toxicology},
   title = {Retinoid-like activity and teratogenic effects of cyanobacterial exudates},
   url = {http://www.sciencedirect.com/science/article/pii/S0166445X14002355},
   volume = {155},
   year = {2014}
}

TY  - JOUR
ID  - 1229158
AU  - Jonáš, Adam - Buráňová, Veronika - Scholz, Stefan - Fetter, Eva - Nováková, Kateřina - Kohoutek, Jiří - Hilscherová, Klára
PY  - 2014
TI  - Retinoid-like activity and teratogenic effects of cyanobacterial exudates
JF  - Aquatic toxicology
VL  - 155
IS  - OCTOBER
SP  - 283-290
EP  - 283-290
PB  - ELSEVIER SCIENCE BV
SN  - 0166445X
KW  - Cyanobacteria
KW  - Developmental toxicity
KW  - Retinoids
KW  - Zebrafish embryo
KW  - All-trans retinoic acid
UR  - http://www.sciencedirect.com/science/article/pii/S0166445X14002355
L2  - http://www.sciencedirect.com/science/article/pii/S0166445X14002355
N2  - Given the essential role of retinoids for vertebrate development this has raised concerns about a potential risk for vertebrates exposed to retinoids during cyanobacterial blooms. Our study focuses on extracellular compounds produced by phytoplankton cells (exudates). In order to address the capacity for the production of retinoids or compounds with retinoid-like activity we compared the exudates of ten cyanobacteria and algae using in vitro reporter gene assay. Exudates of three cyanobacterial species showed retinoid-like activity in the range of 269-2265 ng retinoid equivalents (REQ)/L, while there was no detectable activity in exudates of the investigated algal species. The exudates of one green alga (Desmodesmus quadricaudus) and the two cyanobacterial species with greatest REQ levels, Microgstis aeruginosa and Cylindrospermopsis raciborskii, were selected for testing of the potential relation of retinoid-like activity to developmental toxicity in zebrafish embryos. The exudates of both cyanobacteria were indeed provoking diverse teratogenic effects (e.g. tail, spine and mouth deformation) and interference with growth in zebrafish embryos, while such effects were not observed for the alga. Fish embryos were also exposed to all-trans retinoic acid (ATRA) in a range equivalent to the REQ concentrations detected in exudates by in vitro bioassays. Both the phenotypes and effective concentrations of exudates corresponded to ATRA equivalents, supporting the hypothesis that the teratogenic effects of cyanobacterial exudates are likely to be associated with retinoid-like activity. The study documents that some cyanobacteria are able to produce and release retinoid-like compounds into the environment at concentrations equivalent to those causing teratogenicity in zebrafish. Hence, the characterization of retinoid-like and teratogenic potency should be included in the assessment of the potential adverse effects caused by the release of toxic and bioactive compounds during cyanobacterial blooms.
ER  -

JONÁŠ, Adam; Veronika BURÁŇOVÁ; Stefan SCHOLZ; Eva FETTER; Kateřina NOVÁKOVÁ; Jiří KOHOUTEK a Klára HILSCHEROVÁ. Retinoid-like activity and teratogenic effects of cyanobacterial exudates. \textit{Aquatic toxicology}. AMSTERDAM: ELSEVIER SCIENCE BV, 2014, roč.~155, OCTOBER, s.~283-290. ISSN~0166-445X. Dostupné z: https://dx.doi.org/10.1016/j.aquatox.2014.06.022.

Přehled o publikaci