| ZAVŘEL, Tomáš, Maria, A. SINETOVÁ, Diana BÚZOVÁ, Petra LITERÁKOVÁ a Jan ČERVENÝ. Characterization of a model cyanobacterium Synechocystis sp. PCC 6803 autotrophic growth in a flat-panel photobioreactor. ENGINEERING IN LIFE SCIENCES. WILEY-BLACKWELL, 2015, roč. 15, č. 1, s. 122-132. ISSN 1618-0240. doi:10.1002/elsc.201300165. |
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@article{1229235,
author = {Zavřel, Tomáš and Sinetová, Maria, A. and Búzová, Diana and Literáková, Petra and Červený, Jan},
article_number = {1},
doi = {http://dx.doi.org/10.1002/elsc.201300165},
keywords = {Carbon dioxide; Exponential phase; Growth optimization; Light; Temperature},
language = {eng},
issn = {1618-0240},
journal = {ENGINEERING IN LIFE SCIENCES},
title = {Characterization of a model cyanobacterium Synechocystis sp. PCC 6803 autotrophic growth in a flat-panel photobioreactor.},
volume = {15},
year = {2015}
}
TY - JOUR
ID - 1229235
AU - Zavřel, Tomáš - Sinetová, Maria, A. - Búzová, Diana - Literáková, Petra - Červený, Jan
PY - 2015
TI - Characterization of a model cyanobacterium Synechocystis sp. PCC 6803 autotrophic growth in a flat-panel photobioreactor.
JF - ENGINEERING IN LIFE SCIENCES
VL - 15
IS - 1
SP - 122-132
EP - 122-132
PB - WILEY-BLACKWELL
SN - 16180240
KW - Carbon dioxide
KW - Exponential phase
KW - Growth optimization
KW - Light
KW - Temperature
N2 - We characterized the photoautotrophic growth of glucose-tolerant Synechocystis sp. PCC 6803 in a flat-panel photobioreactor running on a semicontinuous regime under various lights, temperatures, and influx carbon dioxide concentrations. The maximum reached growth rate was 0.135 h(-1), which corresponds to a doubling time of 5.13 ha growth speed never reported for Synechocystis before. Saturating red light intensity for the strain was 220-360 mol(photons)m(-2)s(-1), and we did not observe any photoinhibition up to 660 mol(photons)m(-2)s(-1). Synechocystis was able to grow under red light only; however, photons of wavelengths 405-585 and 670-700 nm further improved its growth. Optimal growth temperature was 35 degrees C. Below 32 degrees C, the growth rates decreased linearly with temperature coefficient (Q(10)) 1.70. Semicontinuous cultivation is known to be efficient for growth characterization and optimization. However, the assumption of correct growth rates calculationculture exponential growthis often not fulfilled. The semicontinuous setup in this study was operated as a turbidostat. Accurate online OD measurements with high time-resolution allowed fast and reliable growth rates determination. Repeating diluting frequencies (up to 18 dilutions per day) were essential for rapid growth stability evaluation. The presented setup provides improvement to previously published semicontinuous characterization strategies by decreasing experimental time requirements and maintaining the culture in exponential growth phase throughout the entire characterization procedure.
ER -
ZAVŘEL, Tomáš, Maria, A. SINETOVÁ, Diana BÚZOVÁ, Petra LITERÁKOVÁ a Jan ČERVENÝ. Characterization of a model cyanobacterium Synechocystis sp. PCC 6803 autotrophic growth in a flat-panel photobioreactor. \textit{ENGINEERING IN LIFE SCIENCES}. WILEY-BLACKWELL, 2015, roč.~15, č.~1, s.~122-132. ISSN~1618-0240. doi:10.1002/elsc.201300165.
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