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ZAVŘEL, Tomáš, Maria, A. SINETOVÁ, Diana BÚZOVÁ, Petra LITERÁKOVÁ a Jan ČERVENÝ. Characterization of a model cyanobacterium Synechocystis sp. PCC 6803 autotrophic growth in a flat-panel photobioreactor. ENGINEERING IN LIFE SCIENCES. WILEY-BLACKWELL, 2015, roč. 15, č. 1, s. 122-132. ISSN 1618-0240. doi:10.1002/elsc.201300165.
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Základní údaje
Originální název Characterization of a model cyanobacterium Synechocystis sp. PCC 6803 autotrophic growth in a flat-panel photobioreactor.
Autoři ZAVŘEL, Tomáš (203 Česká republika, domácí), Maria, A. SINETOVÁ (643 Rusko), Diana BÚZOVÁ (203 Česká republika), Petra LITERÁKOVÁ (203 Česká republika) a Jan ČERVENÝ (203 Česká republika).
Vydání ENGINEERING IN LIFE SCIENCES, WILEY-BLACKWELL, 2015, 1618-0240.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor Biotechnologie a bionika
Stát vydavatele Spojené státy
Utajení není předmětem státního či obchodního tajemství
Impakt faktor Impact factor: 2.168
Kód RIV RIV/00216224:14310/15:00082477
Organizační jednotka Přírodovědecká fakulta
Doi http://dx.doi.org/10.1002/elsc.201300165
UT WoS 000347236300014
Klíčová slova anglicky Carbon dioxide; Exponential phase; Growth optimization; Light; Temperature
Štítky AKR, rivok
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Ing. Andrea Mikešková, učo 137293. Změněno: 25. 4. 2016 14:18.
Anotace
We characterized the photoautotrophic growth of glucose-tolerant Synechocystis sp. PCC 6803 in a flat-panel photobioreactor running on a semicontinuous regime under various lights, temperatures, and influx carbon dioxide concentrations. The maximum reached growth rate was 0.135 h(-1), which corresponds to a doubling time of 5.13 ha growth speed never reported for Synechocystis before. Saturating red light intensity for the strain was 220-360 mol(photons)m(-2)s(-1), and we did not observe any photoinhibition up to 660 mol(photons)m(-2)s(-1). Synechocystis was able to grow under red light only; however, photons of wavelengths 405-585 and 670-700 nm further improved its growth. Optimal growth temperature was 35 degrees C. Below 32 degrees C, the growth rates decreased linearly with temperature coefficient (Q(10)) 1.70. Semicontinuous cultivation is known to be efficient for growth characterization and optimization. However, the assumption of correct growth rates calculationculture exponential growthis often not fulfilled. The semicontinuous setup in this study was operated as a turbidostat. Accurate online OD measurements with high time-resolution allowed fast and reliable growth rates determination. Repeating diluting frequencies (up to 18 dilutions per day) were essential for rapid growth stability evaluation. The presented setup provides improvement to previously published semicontinuous characterization strategies by decreasing experimental time requirements and maintaining the culture in exponential growth phase throughout the entire characterization procedure.
Zobrazeno: 4. 12. 2022 02:48